PMID- 31631634
OWN - NLM
STAT- MEDLINE
DCOM- 20191105
LR  - 20230701
IS  - 1001-5515 (Print)
IS  - 1001-5515 (Linking)
VI  - 36
IP  - 5
DP  - 2019 Oct 25
TI  - [Real-time measurement of cell contractile force during activation of human 
      hepatic stellate cell line LX-2].
PG  - 841-849
LID - 10.7507/1001-5515.201810048 [doi]
AB  - The contractile force of hepatic stellate cells plays a very important role in 
      liver damage, hepatitis and fibrosis. In this paper, a method based on 
      polydimethylsiloxane (PDMS) thin micropillar arrays is proposed to measure the 
      contractile force of human hepatic stellate cell line LX-2, which enables dynamic 
      measurement of the subcellular distribution of force magnitude and direction. 
      First, thin micropillar arrays on glass bottom dish were fabricated using 
      two-step casting process in order to meet the working distance requirement of 
      100x objective lens. After hydrophilic treatment and protein imprint, cells were 
      seeded on the micropillar arrays. LX-2 cells, which were quiesced by growth in 
      serum-free medium, were activated by adding fetal bovine serum (FBS). The 
      deflections of the micropillars were achieved by image processing technique, and 
      then the contractile force of cells exerted on the micropillars was calculated 
      according to mechanical simulation results, and was analyzed under both quiescent 
      and activated conditions. The experimental results show that the average traction 
      force of quiescent cells is about 20 nN, while the contractile force of activated 
      cells increased to 110 nN upon adding FBS. This method can quantify the 
      contractile force of LX-2 cell on subcellular scale in both quiescent and 
      activated states, which may benefit pathology study and drug screen for chronic 
      liver diseases resulted from liver fibrosis.
FAU - Wang, Chunran
AU  - Wang C
AD  - Key Laboratory of the Ministry of Education for Optoelectronic Measurement 
      Technology and Instrument, Beijing Information Science & Technology University, 
      Beijing 100192, P.R.China.
FAU - Zhang, Fan
AU  - Zhang F
AD  - Key Laboratory of the Ministry of Education for Optoelectronic Measurement 
      Technology and Instrument, Beijing Information Science & Technology University, 
      Beijing 100192, P.R.China;Overseas Expertise Introduction Center for Discipline 
      Innovation, Beijing Information Science & Technology University, Beijing 100192, 
      P.R.China.zhangfan@bistu.edu.cn.
FAU - Shan, Baojuan
AU  - Shan B
AD  - Key Laboratory of the Ministry of Education for Optoelectronic Measurement 
      Technology and Instrument, Beijing Information Science & Technology University, 
      Beijing 100192, P.R.China.
FAU - Liu, Jiaqi
AU  - Liu J
AD  - Key Laboratory of the Ministry of Education for Optoelectronic Measurement 
      Technology and Instrument, Beijing Information Science & Technology University, 
      Beijing 100192, P.R.China.
FAU - Zhu, Lianqing
AU  - Zhu L
AD  - Key Laboratory of the Ministry of Education for Optoelectronic Measurement 
      Technology and Instrument, Beijing Information Science & Technology University, 
      Beijing 100192, P.R.China;Overseas Expertise Introduction Center for Discipline 
      Innovation, Beijing Information Science & Technology University, Beijing 100192, 
      P.R.China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
JT  - Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = 
      Shengwu yixue gongchengxue zazhi
JID - 9426398
SB  - IM
MH  - Cell Line
MH  - Hepatic Stellate Cells/*cytology
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Mechanical Phenomena
PMC - PMC9935154
OTO - NOTNLM
OT  - activate
OT  - contractile force
OT  - hepatic stellate cell line
OT  - micropillar arrays
COIS- 利益冲突声明：本文全体作者均声明不存在利益冲突。
EDAT- 2019/10/22 06:00
MHDA- 2019/11/07 06:00
PMCR- 2019/10/01
CRDT- 2019/10/22 06:00
PHST- 2019/10/22 06:00 [entrez]
PHST- 2019/10/22 06:00 [pubmed]
PHST- 2019/11/07 06:00 [medline]
PHST- 2019/10/01 00:00 [pmc-release]
AID - swyxgcxzz-36-5-841 [pii]
AID - 10.7507/1001-5515.201810048 [doi]
PST - ppublish
SO  - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2019 Oct 25;36(5):841-849. doi: 
      10.7507/1001-5515.201810048.