PMID- 31631782
OWN - NLM
STAT- MEDLINE
DCOM- 20200115
LR  - 20200115
IS  - 2222-1751 (Electronic)
IS  - 2222-1751 (Linking)
VI  - 8
IP  - 1
DP  - 2019
TI  - The Nsp12-coding region of type 2 PRRSV is required for viral subgenomic mRNA 
      synthesis.
PG  - 1501-1510
LID - 10.1080/22221751.2019.1679010 [doi]
AB  - As one of many nonstructural proteins of porcine reproductive and respiratory 
      syndrome virus (PRRSV), nonstructural protein 12 (Nsp12) has received relatively 
      little attention, and its role in virus replication, if any, is essentially 
      unknown. By the application of reverse genetic manipulation of an infectious 
      PRRSV clone, the current study is the first to demonstrate that Nsp12 is a key 
      component of PRRSV replication. In addition, the biochemical properties of Nsp12 
      were evaluated, revealing that Nsp12 forms dimers when exposed to oxidative 
      conditions. Furthermore, we systemically analyzed the function of Nsp12 in PRRSV 
      RNA synthesis using a strand-specific PCR method. To our surprise, Nsp12 was not 
      found to be involved in minus-strand genomic RNA (-gRNA) synthesis; importantly, 
      our results indicate that Nsp12 is involved in the synthesis of both plus- and 
      minus-strand subgenomic mRNAs (+sgmRNA and -sgmRNA). Finally, we found that the 
      combination of cysteine 35 and cysteine 79 in Nsp12 is required for sgmRNA 
      synthesis. To our knowledge, we are the first to report the biological role of 
      Nsp12 in the PRRSV lifecycle, and we conclude that Nsp12 is involved in the 
      synthesis of both + sgRNA and -sgRNA.
FAU - Wang, Tong-Yun
AU  - Wang TY
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences , Harbin , People's 
      Republic of China.
FAU - Fang, Qiong-Qiong
AU  - Fang QQ
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences , Harbin , People's 
      Republic of China.
FAU - Cong, Feng
AU  - Cong F
AD  - Guangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals 
      Monitoring Institute , Guangzhou , People's Republic of China.
FAU - Liu, Yong-Gang
AU  - Liu YG
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences , Harbin , People's 
      Republic of China.
FAU - Wang, Hai-Ming
AU  - Wang HM
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences , Harbin , People's 
      Republic of China.
FAU - Zhang, Hong-Liang
AU  - Zhang HL
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences , Harbin , People's 
      Republic of China.
FAU - Tian, Zhi-Jun
AU  - Tian ZJ
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences , Harbin , People's 
      Republic of China.
FAU - Tang, Yan-Dong
AU  - Tang YD
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences , Harbin , People's 
      Republic of China.
FAU - Cai, Xue-Hui
AU  - Cai XH
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences , Harbin , People's 
      Republic of China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Emerg Microbes Infect
JT  - Emerging microbes & infections
JID - 101594885
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Animals
MH  - *Gene Expression Regulation, Viral
MH  - Open Reading Frames
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*metabolism
MH  - RNA, Messenger/*genetics/metabolism
MH  - RNA, Viral/*genetics/metabolism
MH  - Swine
MH  - Transcription, Genetic
MH  - Viral Nonstructural Proteins/genetics/*metabolism
MH  - Virus Replication
PMC - PMC6818116
OTO - NOTNLM
OT  - Nsp12
OT  - PRRSV
OT  - RNA synthesis
OT  - dimer
OT  - subgenomic mRNA
COIS- No potential conflict of interest was reported by the authors.
EDAT- 2019/10/22 06:00
MHDA- 2020/01/16 06:00
PMCR- 2019/10/21
CRDT- 2019/10/22 06:00
PHST- 2019/10/22 06:00 [entrez]
PHST- 2019/10/22 06:00 [pubmed]
PHST- 2020/01/16 06:00 [medline]
PHST- 2019/10/21 00:00 [pmc-release]
AID - 1679010 [pii]
AID - 10.1080/22221751.2019.1679010 [doi]
PST - ppublish
SO  - Emerg Microbes Infect. 2019;8(1):1501-1510. doi: 10.1080/22221751.2019.1679010.