PMID- 31632013
OWN - NLM
STAT- MEDLINE
DCOM- 20191213
LR  - 20231014
IS  - 1178-2013 (Electronic)
IS  - 1176-9114 (Print)
IS  - 1176-9114 (Linking)
VI  - 14
DP  - 2019
TI  - Nano-Hydroxyapatite Coating Promotes Porous Calcium Phosphate Ceramic-Induced 
      Osteogenesis Via BMP/Smad Signaling Pathway.
PG  - 7987-8000
LID - 10.2147/IJN.S216182 [doi]
AB  - BACKGROUND: The hierarchical porous structure and surface topography of calcium 
      phosphate (CaP) bioceramics have a crucial impact on their osteoinductivity. 
      PURPOSE: To fabricate a biomimetic bone graft with an interconnected porous 
      structure analogous to that of trabecular bone and a bioactive nanostructured 
      surface with excellent osteoinductive potential. MATERIALS AND METHODS: A 
      biphasic CaP (BCP) substrate with highly porous structure was fabricated by an 
      improved sponge replication method. Surface modification was performed by 
      uniformly depositing a hydroxyapatite (HA) nanoparticle layer to create 
      nHA-coated BCP scaffolds. The effects of these scaffolds on osteogenic 
      differentiation of murine bone marrow-derived stem cells (BMSCs) were 
      investigated in vitro, and their osteoinductivity was further assessed in vivo. 
      RESULTS: The BCP and nHA-coated BCP scaffolds had similar trabecular bone-like 
      architectures but different surface structures, with mean grain sizes of ~55 nm 
      and ~1 mum, respectively. Compared with the BCP substrate, the nHA-coated BCP 
      scaffolds favored cell adhesion and promoted osteogenic differentiation of BMSCs, 
      as evidenced by upregulated expression of osteogenic genes, enhanced alkaline 
      phosphatase activity, and increased osteocalcin production. This could be 
      attributed to activation of the BMP/Smad signaling pathway, as significantly 
      higher expression levels of BMPRI, Smad1, Smad4, and Smad5 were observed in the 
      nHA-coated BCP group. The nHA-coated BCP scaffold not only maintained scaffold 
      integrity but also induced ectopic bone formation when implanted into rabbit 
      dorsal muscle in vivo for 90 days, whereas the BCP substrate underwent marked 
      biodegradation that led to severe inflammation with no sign of osteogenesis. 
      CONCLUSION: The present study demonstrates the potential of this biomimetic bone 
      graft with a trabecular framework and nanotopography for use in orthopedic 
      applications.
CI  - (c) 2019 Wang et al.
FAU - Wang, Jing
AU  - Wang J
AD  - National Engineering Research Center for Biomaterials, Sichuan University, 
      Chengdu 610064, People's Republic of China.
FAU - Wang, Menglu
AU  - Wang M
AD  - National Engineering Research Center for Biomaterials, Sichuan University, 
      Chengdu 610064, People's Republic of China.
FAU - Chen, Fuying
AU  - Chen F
AD  - National Engineering Research Center for Biomaterials, Sichuan University, 
      Chengdu 610064, People's Republic of China.
FAU - Wei, Yihang
AU  - Wei Y
AD  - National Engineering Research Center for Biomaterials, Sichuan University, 
      Chengdu 610064, People's Republic of China.
FAU - Chen, Xuening
AU  - Chen X
AD  - National Engineering Research Center for Biomaterials, Sichuan University, 
      Chengdu 610064, People's Republic of China.
FAU - Zhou, Yong
AU  - Zhou Y
AD  - Department of Orthopaedics, West China Hospital of Sichuan University, Chengdu 
      610041, People's Republic of China.
FAU - Yang, Xiao
AU  - Yang X
AD  - National Engineering Research Center for Biomaterials, Sichuan University, 
      Chengdu 610064, People's Republic of China.
FAU - Zhu, Xiangdong
AU  - Zhu X
AUID- ORCID: 0000-0002-3818-7419
AD  - National Engineering Research Center for Biomaterials, Sichuan University, 
      Chengdu 610064, People's Republic of China.
FAU - Tu, Chongqi
AU  - Tu C
AUID- ORCID: 0000-0002-4576-4712
AD  - Department of Orthopaedics, West China Hospital of Sichuan University, Chengdu 
      610041, People's Republic of China.
FAU - Zhang, Xingdong
AU  - Zhang X
AD  - National Engineering Research Center for Biomaterials, Sichuan University, 
      Chengdu 610064, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20191003
PL  - New Zealand
TA  - Int J Nanomedicine
JT  - International journal of nanomedicine
JID - 101263847
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Calcium Phosphates)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Smad Proteins)
RN  - 91D9GV0Z28 (Durapatite)
RN  - 97Z1WI3NDX (calcium phosphate)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Biomimetic Materials/pharmacology
MH  - Bone Morphogenetic Proteins/*metabolism
MH  - Calcium Phosphates/*pharmacology
MH  - Cell Adhesion/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cell Shape/drug effects
MH  - Ceramics/*pharmacology
MH  - Choristoma/pathology
MH  - Coated Materials, Biocompatible/*pharmacology
MH  - Durapatite/chemistry/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Male
MH  - Mesenchymal Stem Cells/cytology/drug effects/enzymology/ultrastructure
MH  - Mice
MH  - Nanoparticles/*chemistry/ultrastructure
MH  - Osteogenesis/*drug effects/genetics
MH  - Porosity
MH  - Rabbits
MH  - Signal Transduction/drug effects
MH  - Smad Proteins/*metabolism
MH  - Tissue Scaffolds/chemistry
PMC - PMC6781424
OTO - NOTNLM
OT  - MSCs
OT  - calcium phosphate ceramics
OT  - nano-hydroxyapatite
OT  - osteoblastic differentiation
OT  - osteoinduction
OT  - porous scaffolds
COIS- The authors report no conflicts of interest in this work.
EDAT- 2019/10/22 06:00
MHDA- 2019/12/18 06:00
PMCR- 2019/10/03
CRDT- 2019/10/22 06:00
PHST- 2019/05/17 00:00 [received]
PHST- 2019/09/17 00:00 [accepted]
PHST- 2019/10/22 06:00 [entrez]
PHST- 2019/10/22 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/10/03 00:00 [pmc-release]
AID - 216182 [pii]
AID - 10.2147/IJN.S216182 [doi]
PST - epublish
SO  - Int J Nanomedicine. 2019 Oct 3;14:7987-8000. doi: 10.2147/IJN.S216182. 
      eCollection 2019.