PMID- 31632101
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220410
IS  - 1178-6973 (Print)
IS  - 1178-6973 (Electronic)
IS  - 1178-6973 (Linking)
VI  - 12
DP  - 2019
TI  - Characterization Of The Interaction Between Subviral Particles Of Hepatitis B 
      Virus And Dendritic Cells - In Vitro Study.
PG  - 3125-3135
LID - 10.2147/IDR.S221294 [doi]
AB  - BACKGROUND: During an infection with hepatitis B virus (HBV), infectious 
      particles (Dane particles) can be detected in addition to aggregates of the 
      subviral particles (SVP) which is considered an immune escaping mechanism for the 
      virus. Dendritic cells (DCs) are a specialized type of antigen-presenting cell 
      (APC) that can activate native T-cells to prime an immune response controlling 
      HBV infection. The aim of this study was to characterize the interaction between 
      HBVsvp and DCs in vitro. METHODS: HBVsvp that comprises surface and core proteins 
      were produced in vitro by HepG2.2.15 as a culturing system; DCs derived from the 
      bone marrow of mice were pulsed by HBVsvp. A different pattern of cytokines 
      secreted by bone-marrow-derived dendritic cells from C56BL/6 mice pulsed with 
      HBVsvp were analyzed. The interactions between HBVsvp and DCs were characterized 
      using FACS analysis, protein assay, Western blot, and immunofluorescence 
      staining. RESULTS: Pulsation of DCs with HBVsvp resulted in strong activation and 
      higher secretion of DC cytokines including INF-alpha, INF-gamma, TNF-alpha, IL-1alpha, IL-10, and 
      IL-12; but not for IL-1beta, IL-2, IL-6, and IL-15. The production of CXCL-10/IP-10 
      was increased during the observation period and reached the maximal secretion 
      after 24 hrs (p < 0.001). In total protein assay, we found significantly higher 
      protein concentration in HBVsvp stimulated DC groups compared to not activated 
      DCs (p < 0.001). Both 24 kDa small surface antigen (HBVs) and the 21 kDa core 
      protein (HBVc) were detected in activated DCs. For DCs immunofluorescence 
      staining, our data showed clear differences in the morphology of DCs between 
      negative control and those pulsed with HBVsvp. CONCLUSION: Result demonstrates a 
      significant complex interaction between HBVsvp and DCs, in vitro.
CI  - (c) 2019 Farag et al.
FAU - Farag, Mohamed Ms
AU  - Farag MM
AUID- ORCID: 0000-0003-0581-8576
AD  - Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr 
      City, Cairo, Egypt.
FAU - Peschel, Georg
AU  - Peschel G
AD  - Department of Gastroenterology, Endocrinology, Rheumatology and Infectious 
      Diseases, University Hospital Regensburg, Regensburg 93053, Germany.
FAU - Muller, Martina
AU  - Muller M
AD  - Department of Gastroenterology, Endocrinology, Rheumatology and Infectious 
      Diseases, University Hospital Regensburg, Regensburg 93053, Germany.
FAU - Weigand, Kilian
AU  - Weigand K
AD  - Department of Gastroenterology, Endocrinology, Rheumatology and Infectious 
      Diseases, University Hospital Regensburg, Regensburg 93053, Germany.
LA  - eng
PT  - Journal Article
DEP - 20191007
PL  - New Zealand
TA  - Infect Drug Resist
JT  - Infection and drug resistance
JID - 101550216
PMC - PMC6789970
OTO - NOTNLM
OT  - cytokines
OT  - dendritic cell-based therapy
OT  - hepatitis B virus subviral particles
COIS- The authors report no conflicts of interest in this work.
EDAT- 2019/10/22 06:00
MHDA- 2019/10/22 06:01
PMCR- 2019/10/07
CRDT- 2019/10/22 06:00
PHST- 2019/06/29 00:00 [received]
PHST- 2019/08/28 00:00 [accepted]
PHST- 2019/10/22 06:00 [entrez]
PHST- 2019/10/22 06:00 [pubmed]
PHST- 2019/10/22 06:01 [medline]
PHST- 2019/10/07 00:00 [pmc-release]
AID - 221294 [pii]
AID - 10.2147/IDR.S221294 [doi]
PST - epublish
SO  - Infect Drug Resist. 2019 Oct 7;12:3125-3135. doi: 10.2147/IDR.S221294. 
      eCollection 2019.