PMID- 31632243
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1662-5102 (Print)
IS  - 1662-5102 (Electronic)
IS  - 1662-5102 (Linking)
VI  - 13
DP  - 2019
TI  - Two Preputial Gland-Secreted Pheromones Evoke Sexually Dimorphic Neural Pathways 
      in the Mouse Vomeronasal System.
PG  - 455
LID - 10.3389/fncel.2019.00455 [doi]
LID - 455
AB  - Hexadecanol (16OH) and hexadecyl acetate (16Ac) are two pheromones secreted in a 
      large quantity by mouse preputial glands and act on male and female mice 
      differentially. Yet the underlying molecular and cellular mechanisms remain to be 
      elucidated. In this study, we examined the activation of vomeronasal sensory 
      neurons (VSNs) by these two pheromones and mapped the downstream neural circuits 
      that process and relay their chemosignals. Using the calcium imaging method and 
      immunohistochemistry, we found that a small number of VSNs were activated by 
      16OH, 16AC, or both in the male and female mice, most of which were located 
      apically in the vomeronasal epithelium, and their numbers did not increase when 
      the concentrations of 16OH and 16Ac were raised by 10,000-fold except that of 
      female VSNs in response to 16OH. In the accessory olfactory bulb (AOB), the two 
      pheromones evoked more c-Fos+ neurons in the anterior AOB (aAOB) than in the 
      posterior AOB (pAOB); and the increases in the number of c-Fos+ neurons in both 
      aAOB and pAOB were dose-dependent; and between sexes, the female AOB responded 
      more strongly to 16OH than to 16Ac whereas the male AOB had the opposite response 
      pattern. This sexual dimorphism was largely retained in the downstream brain 
      regions, including the bed nucleus of the stria terminalis (BNST), the medial 
      amygdaloid nucleus (MeA), the posteromedial cortical amygdaloid nucleus (PMCo), 
      the medial preoptic area (MPA), and the ventromedial hypothalamic nucleus (VmH). 
      Taken together, out data indicate that there is one V1r receptor each for 16OH, 
      16Ac, or both, and that activation of these receptors evokes sexually dimorphic 
      neural circuits, directing different behavioral outputs and possibly modulating 
      other pheromone-induced responses.
CI  - Copyright (c) 2019 Liu, Zhang, Wang, Guo, Wu, Zhang and Huang.
FAU - Liu, Qun
AU  - Liu Q
AD  - College of Life Sciences, Zhejiang University, Hangzhou, China.
FAU - Zhang, Yaohua
AU  - Zhang Y
AD  - State Key Laboratory of Integrated Management of Pest Insects and Rodents in 
      Agriculture, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
FAU - Wang, Pan
AU  - Wang P
AD  - State Key Laboratory of Integrated Management of Pest Insects and Rodents in 
      Agriculture, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
FAU - Guo, Xiao
AU  - Guo X
AD  - State Key Laboratory of Integrated Management of Pest Insects and Rodents in 
      Agriculture, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
FAU - Wu, Yijun
AU  - Wu Y
AD  - State Key Laboratory of Integrated Management of Pest Insects and Rodents in 
      Agriculture, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
FAU - Zhang, Jian-Xu
AU  - Zhang JX
AD  - State Key Laboratory of Integrated Management of Pest Insects and Rodents in 
      Agriculture, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
FAU - Huang, Liquan
AU  - Huang L
AD  - College of Life Sciences, Zhejiang University, Hangzhou, China.
AD  - Monell Chemical Senses Center, Philadelphia, PA, United States.
LA  - eng
PT  - Journal Article
DEP - 20191002
PL  - Switzerland
TA  - Front Cell Neurosci
JT  - Frontiers in cellular neuroscience
JID - 101477935
PMC - PMC6783556
OTO - NOTNLM
OT  - c-Fos
OT  - calcium imaging
OT  - hexadecanol
OT  - hexadecyl acetate
OT  - neural circuits
OT  - vomeronasal organ
EDAT- 2019/10/22 06:00
MHDA- 2019/10/22 06:01
PMCR- 2019/01/01
CRDT- 2019/10/22 06:00
PHST- 2019/07/23 00:00 [received]
PHST- 2019/09/24 00:00 [accepted]
PHST- 2019/10/22 06:00 [entrez]
PHST- 2019/10/22 06:00 [pubmed]
PHST- 2019/10/22 06:01 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fncel.2019.00455 [doi]
PST - epublish
SO  - Front Cell Neurosci. 2019 Oct 2;13:455. doi: 10.3389/fncel.2019.00455. 
      eCollection 2019.