PMID- 31633807
OWN - NLM
STAT- MEDLINE
DCOM- 20200122
LR  - 20200122
IS  - 1099-0801 (Electronic)
IS  - 0269-3879 (Linking)
VI  - 34
IP  - 1
DP  - 2020 Jan
TI  - Development of a gas chromatography-mass spectrometry method for breast cancer 
      diagnosis based on nucleoside metabolomes 1-methyl adenosine, 1-methylguanosine 
      and 8-hydroxy-2'-deoxyguanosine.
PG  - e4713
LID - 10.1002/bmc.4713 [doi]
AB  - Metabolomes are small molecule metabolites (<1000 Da) produced by cellular 
      processes. Metabolomes are close counterparts to the genome, transcriptome and 
      proteome. The aim of this study was to develop a method to detect and quantify 
      candidate nucleoside metabolomes 1-methyl adenosine (1-MA), 1-methylguanosine 
      (1-MG) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in the urine of patients with 
      breast cancer using gas chromatography-mass spectrometry (GC-MS). The method was 
      applied to urine specimens from patients with breast cancer (n = 56) and benign 
      breast tumors (n = 22), as well as from healthy females (n = 20). The relative 
      standard deviations of precision and repeatability analysis were <10%, and 
      recoveries ranged from 88.5 to 105.6%. Limits of detection were 0.014, 0.012, and 
      0.018 mg/L for 1-MA, 1-MG and 8-OHdG, respectively. The lower limits of 
      quantitation were 0.056, 0.048 and 0.072 mg/L, respectively. There were 
      significant differences in concentrations of candidate metabolomes between 
      patients with cancer and the healthy individuals, especially for those in the 
      early stages of the disease (p < 0.001). No significant differences were observed 
      between the benign and healthy groups. In conclusion, a reliable GC-MS method for 
      the detection and quantification of 1-MA, 1-MG, and 8-OHdG metabolomes in urine 
      has been developed.
CI  - (c) 2019 John Wiley & Sons, Ltd.
FAU - Omran, Mohamed M
AU  - Omran MM
AUID- ORCID: 0000-0001-8557-3104
AD  - Chemistry Department, Faculty of Science-Helwan University, Cairo, Egypt.
FAU - Rashed, Ramzy E
AU  - Rashed RE
AUID- ORCID: 0000-0002-2517-0976
AD  - Laboratory of Toxicology, Central Laboratories, Ministry of Health, Damietta, 
      Egypt.
FAU - Darwish, Hossam
AU  - Darwish H
AD  - Damietta Cancer Institute, Damietta/Head of medical oncology department, Ismailia 
      Teaching Oncology Hospital, Ismailia, Egypt.
FAU - Belal, Arafa A
AU  - Belal AA
AD  - Chemistry Department, Faculty of Science, Port Said University, Port Said, Egypt.
FAU - Mohamed, Faten Z
AU  - Mohamed FZ
AD  - Chemistry Department, Faculty of Science, Zagazig University, Zagazig, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20191123
PL  - England
TA  - Biomed Chromatogr
JT  - Biomedical chromatography : BMC
JID - 8610241
RN  - 12133JR80S (Guanosine)
RN  - 15763-06-1 (1-methyladenosine)
RN  - K72T3FS567 (Adenosine)
SB  - IM
MH  - Adenosine/*analogs & derivatives/chemistry/urine
MH  - Adult
MH  - Aged
MH  - Breast Neoplasms/metabolism/*urine
MH  - Female
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - *Guanosine/analogs & derivatives/chemistry/urine
MH  - Humans
MH  - Limit of Detection
MH  - Linear Models
MH  - Metabolome/physiology
MH  - Metabolomics/*methods
MH  - Middle Aged
MH  - Reproducibility of Results
OTO - NOTNLM
OT  - breast cancer
OT  - gas chromatography
OT  - mass spectrometry
OT  - metabolome
OT  - metabolomics
EDAT- 2019/10/22 06:00
MHDA- 2020/01/23 06:00
CRDT- 2019/10/22 06:00
PHST- 2019/03/27 00:00 [received]
PHST- 2019/09/15 00:00 [revised]
PHST- 2019/09/30 00:00 [accepted]
PHST- 2019/10/22 06:00 [pubmed]
PHST- 2020/01/23 06:00 [medline]
PHST- 2019/10/22 06:00 [entrez]
AID - 10.1002/bmc.4713 [doi]
PST - ppublish
SO  - Biomed Chromatogr. 2020 Jan;34(1):e4713. doi: 10.1002/bmc.4713. Epub 2019 Nov 23.