PMID- 31635396
OWN - NLM
STAT- MEDLINE
DCOM- 20200316
LR  - 20231019
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 11
IP  - 10
DP  - 2019 Oct 18
TI  - Vitamin D Improves Nitric Oxide-Dependent Vasodilation in Adipose Tissue 
      Arterioles from Bariatric Surgery Patients.
LID - 10.3390/nu11102521 [doi]
LID - 2521
AB  - There is a high prevalence of vitamin-D deficiency in obese individuals that 
      could be attributed to vitamin-D sequestration in the adipose tissue. 
      Associations between vitamin-D deficiency and unfavorable cardiometabolic 
      outcomes were reported. However, the pathophysiological mechanisms behind these 
      associations are yet to be established. In our previous studies, we demonstrated 
      microvascular dysfunction in obese adults that was associated with reduced nitric 
      oxide (NO) production. Herein, we examined the role of vitamin D in mitigating 
      microvascular function in morbidly obese adults before and after weight loss 
      surgery. We obtained subcutaneous (SAT) and visceral adipose tissue (VAT) 
      biopsies from bariatric patients at the time of surgery (n = 15) and gluteal SAT 
      samples three months post-surgery (n = 8). Flow-induced dilation (FID) and 
      acetylcholine-induced dilation (AChID) and NO production were measured in the 
      AT-isolated arterioles +/- NO synthase inhibitor N(omega)-nitro-L-arginine methyl ester 
      (L-NAME), hydrogen peroxide (H(2)O(2)) inhibitor, polyethylene glycol-modified 
      catalase (PEG-CAT), or 1,25-dihydroxyvitamin D. Vitamin D improved FID, AChID, 
      and NO production in AT-isolated arterioles at time of surgery; these effects 
      were abolished by L-NAME but not by PEG-CAT. Vitamin-D-mediated improvements were 
      of a higher magnitude in VAT compared to SAT arterioles. After surgery, 
      significant improvements in FID, AChID, NO production, and NO sensitivity were 
      observed. Vitamin-D-induced changes were of a lower magnitude compared to those 
      from the time of surgery. In conclusion, vitamin D improved NO-dependent 
      arteriolar vasodilation in obese adults; this effect was more significant before 
      surgery-induced weight loss.
FAU - Mahmoud, Abeer M
AU  - Mahmoud AM
AD  - Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, 
      University of Illinois at Chicago, Chicago, IL 60612, USA. amahmo4@uic.edu.
AD  - Department of Physical Therapy, University of Illinois at Chicago, Chicago, IL 
      60612, USA. amahmo4@uic.edu.
AD  - Integrative Physiology Laboratory, College of Applied Health Sciences, University 
      of Illinois at Chicago, Chicago, IL 60612, USA. amahmo4@uic.edu.
FAU - Szczurek, Mary
AU  - Szczurek M
AD  - Department of Physical Therapy, University of Illinois at Chicago, Chicago, IL 
      60612, USA. mszczurek@uic.edu.
FAU - Hassan, Chandra
AU  - Hassan C
AD  - Department of Surgery, University of Illinois at Chicago, Chicago, IL 60612, USA. 
      chandrar@uic.edu.
FAU - Masrur, Mario
AU  - Masrur M
AD  - Department of Surgery, University of Illinois at Chicago, Chicago, IL 60612, USA. 
      mmasrur@uic.edu.
FAU - Gangemi, Antonio
AU  - Gangemi A
AD  - Department of Surgery, University of Illinois at Chicago, Chicago, IL 60612, USA. 
      gangemi@uic.edu.
FAU - Phillips, Shane A
AU  - Phillips SA
AD  - Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, 
      University of Illinois at Chicago, Chicago, IL 60612, USA. shanep@uic.edu.
AD  - Department of Physical Therapy, University of Illinois at Chicago, Chicago, IL 
      60612, USA. shanep@uic.edu.
AD  - Integrative Physiology Laboratory, College of Applied Health Sciences, University 
      of Illinois at Chicago, Chicago, IL 60612, USA. shanep@uic.edu.
LA  - eng
GR  - K99 HL140049/HL/NHLBI NIH HHS/United States
GR  - HL095701/NH/NIH HHS/United States
GR  - HL130513/NH/NIH HHS/United States
GR  - K99HL140049/NH/NIH HHS/United States
GR  - 4 R00 HL140049-03/NH/NIH HHS/United States
GR  - R00 HL140049/HL/NHLBI NIH HHS/United States
GR  - UL1 TR002003/TR/NCATS NIH HHS/United States
PT  - Journal Article
DEP - 20191018
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 1406-16-2 (Vitamin D)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Adipose Tissue/*blood supply
MH  - Adult
MH  - Arterioles/*drug effects
MH  - Bariatric Surgery
MH  - Female
MH  - Humans
MH  - Hydrogen Peroxide
MH  - Male
MH  - Middle Aged
MH  - Nitric Oxide/*metabolism/pharmacology
MH  - Tissue Culture Techniques
MH  - Vasodilation/*drug effects
MH  - Vitamin D/*pharmacology
MH  - Young Adult
PMC - PMC6835261
OTO - NOTNLM
OT  - bariatric surgery
OT  - microvascular
OT  - nitric oxide
OT  - obesity
OT  - vitamin D
OT  - weight loss
COIS- The authors declare no conflict of interest.
EDAT- 2019/10/23 06:00
MHDA- 2020/03/17 06:00
PMCR- 2019/10/01
CRDT- 2019/10/23 06:00
PHST- 2019/09/06 00:00 [received]
PHST- 2019/10/14 00:00 [revised]
PHST- 2019/10/15 00:00 [accepted]
PHST- 2019/10/23 06:00 [entrez]
PHST- 2019/10/23 06:00 [pubmed]
PHST- 2020/03/17 06:00 [medline]
PHST- 2019/10/01 00:00 [pmc-release]
AID - nu11102521 [pii]
AID - nutrients-11-02521 [pii]
AID - 10.3390/nu11102521 [doi]
PST - epublish
SO  - Nutrients. 2019 Oct 18;11(10):2521. doi: 10.3390/nu11102521.