PMID- 31635774
OWN - NLM
STAT- MEDLINE
DCOM- 20200309
LR  - 20200309
IS  - 1873-2232 (Electronic)
IS  - 0378-4320 (Linking)
VI  - 210
DP  - 2019 Nov
TI  - Effects of Isatis root polysaccharide on boar sperm quality during liquid storage 
      and in vitro fertilization.
PG  - 106178
LID - S0378-4320(19)30348-3 [pii]
LID - 10.1016/j.anireprosci.2019.106178 [doi]
AB  - Liquid preservation of boar semen is a preferred method in pig husbandry, and 
      antioxidants to protect against sperm oxidative stress during periods of storage 
      have become the focus of recent research. Through its antioxidant activity, 
      Isatis root polysaccharide (IRPS), a plant extract, can effectively reduce the 
      cellular lipid peroxidation caused by the accumulation of reactive oxygen species 
      inside mitochondria. In the present study, there was examination of the effects 
      of no supplementation (Control) of a semen extender with or supplementation in 
      different concentrations of IRPS (0.2, 0.4, 0.6, 0.8, and 1.2 mg/mL) on sperm 
      quality variables and antioxidant capacity during liquid storage. The results 
      indicate that after prolonged storage (>/= 3 days), the sperm motility was greater 
      in the group supplemented with 0.6 mg/mL IRPS than in the other groups (P <  
      0.05). The use of this IRPS concentration also resulted in maintanence of 
      acrosome integrity, plasma membrane integrity, mitochondrial membrane potential, 
      and antioxidant capacity of the sperm (P <  0.05). Furthermore, the results of an 
      in vitro fertilization study indicate IRPS at 0.6 mg/mL markedly increased the 
      sperm fertilization capacity (P <  0.01) and embryonic development to the 
      blastocyst stage (P <  0.05). The addition of 0.6 mg/mL IRPS enhanced the 
      antioxidant capacity of boar sperm, resulting in greater preservation of sperm 
      motility and fertilization capacity during liquid storage. These findings 
      indicate that IRPS has the potential to be used as a component of a 
      semen-preserving diluent to maintain sperm quality during storage.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Ren, Zhiqiang
AU  - Ren Z
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, 
      College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China.
FAU - Shaoyong, Weike
AU  - Shaoyong W
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, 
      College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China.
FAU - Li, Qian
AU  - Li Q
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, 
      College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China.
FAU - Ma, Lu
AU  - Ma L
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, 
      College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China.
FAU - Xiao, Junying
AU  - Xiao J
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, 
      College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China.
FAU - Jiao, Junheng
AU  - Jiao J
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, 
      College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China.
FAU - Yang, Gongshe
AU  - Yang G
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, 
      College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China.
FAU - Pang, Weijun
AU  - Pang W
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, 
      College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China. Electronic address: pwj1226@nwsuaf.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190905
PL  - Netherlands
TA  - Anim Reprod Sci
JT  - Animal reproduction science
JID - 7807205
RN  - 0 (Plant Extracts)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Animals
MH  - Cell Membrane/drug effects
MH  - Fertilization in Vitro/veterinary
MH  - Isatis/*chemistry
MH  - Male
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Plant Roots/*chemistry
MH  - Polysaccharides/chemistry/*pharmacology
MH  - Semen Preservation/veterinary
MH  - Sperm Motility/drug effects
MH  - Spermatozoa/*drug effects
MH  - *Swine
OTO - NOTNLM
OT  - Boar sperm
OT  - IVF
OT  - Isatis root polysaccharide
OT  - Liquid storage
EDAT- 2019/10/23 06:00
MHDA- 2020/03/10 06:00
CRDT- 2019/10/23 06:00
PHST- 2019/04/12 00:00 [received]
PHST- 2019/08/12 00:00 [revised]
PHST- 2019/09/04 00:00 [accepted]
PHST- 2019/10/23 06:00 [entrez]
PHST- 2019/10/23 06:00 [pubmed]
PHST- 2020/03/10 06:00 [medline]
AID - S0378-4320(19)30348-3 [pii]
AID - 10.1016/j.anireprosci.2019.106178 [doi]
PST - ppublish
SO  - Anim Reprod Sci. 2019 Nov;210:106178. doi: 10.1016/j.anireprosci.2019.106178. 
      Epub 2019 Sep 5.