PMID- 31636507
OWN - NLM
STAT- MEDLINE
DCOM- 20200406
LR  - 20200408
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2019
DP  - 2019
TI  - Trypanosoma cruzi Mexican Strains Differentially Modulate Surface Markers and 
      Cytokine Production in Bone Marrow-Derived Dendritic Cells from C57BL/6 and 
      BALB/c Mice.
PG  - 7214798
LID - 10.1155/2019/7214798 [doi]
LID - 7214798
AB  - Dendritic cells (DCs) are a type of antigen-presenting cells that play an 
      important role in the immune response against Trypanosoma cruzi, the causative 
      agent of Chagas disease. In vitro and in vivo studies have shown that the 
      modulation of these cells by this parasite can directly affect the innate and 
      acquired immune response of the host in order to facilitate its biological cycle 
      and the spreading of the species. Many studies show the mechanisms by which T. 
      cruzi modulates DCs, but the interaction of these cells with the Mexican strains 
      of T. cruzi such as Ninoa and INC5 has not yet been properly investigated. Here, 
      we evaluated whether Ninoa and INC5 strains evaded the immunity of their hosts by 
      modulating the biology and function of murine DCs. The CL-Brener strain was used 
      as the reference strain. Herein, it was demonstrated that Ninoa was more 
      infective toward bone marrow-derived dendritic cells (BMDCs) than INC5 and 
      CL-Brener strains in both BMDCs of BALB/c and C57BL/6 mice. Mexican strains of T. 
      cruzi induced different cytokine patterns. In BMDCs obtained from BALB/c mice, 
      Ninoa strain led to the reduction in IL-6 and increased IL-10 production, while 
      in C57BL/6 mice Ninoa strain considerably increased the productions of TNF-alpha and 
      IL-10. Also, Ninoa and INC5 differentially modulated BMDC expressions of MHC-II, 
      TLR2, and TLR4 in both BALB/c and C57BL/6 mice compared to Brazilian strain 
      CL-Brener. These results indicate that T. cruzi Mexican strains differentially 
      infect and modulate MHC-II, toll-like receptors, and cytokine production in DCs 
      obtained from C57BL/6 and BALB/c mice, suggesting that these strains have 
      developed particular modulatory strategies to disrupt DCs and, consequently, the 
      host immune responses.
CI  - Copyright (c) 2019 Cecilia Gomes Barbosa et al.
FAU - Barbosa, Cecilia Gomes
AU  - Barbosa CG
AD  - Laboratory of Parasitology, Department of Immunology, Microbiology and 
      Parasitology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - Carvalho Costa, Tamires Marielem
AU  - Carvalho Costa TM
AD  - Laboratory of Immunology, Department of Immunology, Microbiology and 
      Parasitology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - Desiderio, Chamberttan Souza
AU  - Desiderio CS
AD  - Laboratory of Immunology, Department of Immunology, Microbiology and 
      Parasitology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - Ferreira, Paula Tatiana Mutao
AU  - Ferreira PTM
AD  - Laboratory of Immunology, Department of Immunology, Microbiology and 
      Parasitology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - Silva, Mariana de Oliveira
AU  - Silva MO
AD  - Laboratory of Parasitology, Department of Immunology, Microbiology and 
      Parasitology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - Hernandez, Cesar Gomez
AU  - Hernandez CG
AUID- ORCID: 0000-0003-2792-8485
AD  - Laboratory of Immunology, Department of Immunology, Microbiology and 
      Parasitology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - Santos, Malu Mateus
AU  - Santos MM
AD  - Laboratory of Immunology, Department of Immunology, Microbiology and 
      Parasitology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - Trevisan, Rafael Obata
AU  - Trevisan RO
AD  - Laboratory of Immunology, Department of Immunology, Microbiology and 
      Parasitology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - Bovi, Weslley Guimaraes
AU  - Bovi WG
AUID- ORCID: 0000-0002-7737-1737
AD  - Laboratory of Immunology, Department of Immunology, Microbiology and 
      Parasitology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - Rodrigues, Virmondes
AU  - Rodrigues V
AUID- ORCID: 0000-0001-8706-4223
AD  - Laboratory of Immunology, Department of Immunology, Microbiology and 
      Parasitology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - Machado, Juliana Reis
AU  - Machado JR
AUID- ORCID: 0000-0002-0471-1454
AD  - Department of General Pathology, Federal University of Triangulo Mineiro, 
      Uberaba, MG, Brazil.
FAU - Ramirez, Luiz Eduardo
AU  - Ramirez LE
AD  - Laboratory of Parasitology, Department of Immunology, Microbiology and 
      Parasitology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - de Oliveira, Carlo Jose Freire
AU  - de Oliveira CJF
AUID- ORCID: 0000-0003-2211-7333
AD  - Laboratory of Immunology, Department of Immunology, Microbiology and 
      Parasitology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - da Silva, Marcos Vinicius
AU  - da Silva MV
AUID- ORCID: 0000-0002-2966-7621
AD  - Laboratory of Parasitology, Department of Immunology, Microbiology and 
      Parasitology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20190915
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*metabolism
MH  - Cytokines/*metabolism
MH  - Dendritic Cells/*metabolism
MH  - Interleukin-10/metabolism
MH  - Interleukin-6/metabolism
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Toll-Like Receptor 2/metabolism
MH  - Toll-Like Receptor 4/metabolism
MH  - Trypanosoma cruzi/*pathogenicity
PMC - PMC6766131
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2019/10/23 06:00
MHDA- 2020/04/09 06:00
PMCR- 2019/09/15
CRDT- 2019/10/23 06:00
PHST- 2019/03/16 00:00 [received]
PHST- 2019/06/08 00:00 [revised]
PHST- 2019/07/29 00:00 [accepted]
PHST- 2019/10/23 06:00 [entrez]
PHST- 2019/10/23 06:00 [pubmed]
PHST- 2020/04/09 06:00 [medline]
PHST- 2019/09/15 00:00 [pmc-release]
AID - 10.1155/2019/7214798 [doi]
PST - epublish
SO  - Mediators Inflamm. 2019 Sep 15;2019:7214798. doi: 10.1155/2019/7214798. 
      eCollection 2019.