PMID- 31638565
OWN - NLM
STAT- MEDLINE
DCOM- 20191104
LR  - 20200108
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 35
IP  - 8
DP  - 2019 Aug
TI  - [Knockout of TLR2 gene attenuates insulin resistance and promotes M2 polarization 
      of macrophages in mice].
PG  - 689-694
AB  - Objective To study the effect of deletion of Toll-like receptor 2 (TLR2) gene on 
      insulin resistance and polarization of macrophages in mice. Methods The wild-type 
      (WT) and TLR2 knockout (TLR2(-/-)) C57BL/6 male mice, aged 28 days, were 
      selected, with 12 mice in each group. The genotype of each mouse was identified 
      by PCR. After mice were fed with basic diet for 3 months, the glucose tolerance 
      test (GTT) and insulin resistance test (ITT) were performed. The mononuclear 
      cells isolated from peripheral blood were stimulated with GM-CSF/IFN-gamma and 
      M-CSF/IL-4/IL-13, respectively, to induce differentiation to M1-like and M2-like 
      macrophages. The CD11b, F4/80, CD11c, CD206 and early growth response 2 (EGR2) 
      were detected by flow cytometry to determine the phenotype of macrophages. The 
      levels of TNF-alpha, IL-6 and IL-10 in the culture supernatant of macrophages were 
      detected using ELISA. Results The result of PCR identification was consistent 
      with the genotype of mice. Compared to WT mice, TLR2(-/-) mice exhibited the 
      significantly improved glycemic control at 30 min during GTT and the 
      significantly increased insulin sensitivity at 15 minutes during ITT. The flow 
      cytometry showed that M1 markers decreased and M2 macrophages increased in the 
      TLR2(-/-) mice. ELISA showed that the levels of IL-6 and TNF-alpha significantly 
      decreased in the culture supernatant of M1 macrophages, while the level of IL-10 
      significantly increased in the culture supernatant of M2 macrophages in TLR2(-/-) 
      mice compared with WT mice. Conclusion TLR2 signal has an effect on the 
      polarization of macrophages and makes macrophages tend to switch to M1 phenotype. 
      A higher number of pro-inflammatory factors secreted by M1 macrophages contribute 
      to a low-grade inflammation state in the body, which leads to a decrease in 
      glucose tolerance and insulin sensitivity.
FAU - Ma, Yu
AU  - Ma Y
AD  - Key Laboratory of Clinical Diagnostics, Hebei North University, Zhangjiakou 
      075000, China.
FAU - Guo, Hao
AU  - Guo H
AD  - Key Laboratory of Clinical Diagnostics, Hebei North University, Zhangjiakou 
      075000, China.
FAU - Wu, Yaliu
AU  - Wu Y
AD  - Key Laboratory of Clinical Diagnostics, Hebei North University, Zhangjiakou 
      075000, China.
FAU - Geng, Hemei
AU  - Geng H
AD  - Department of Infection Management, Kailuan General Hospital, Hebei United 
      University, Tangshan 063000, China.
FAU - Zheng, Lining
AU  - Zheng L
AD  - Grade 2015, Medical Laboratory of North University of Hebei, Zhangjiakou 075000, 
      China.
FAU - Wang, Wendong
AU  - Wang W
AD  - Key Laboratory of Clinical Diagnostics, Hebei North University, Zhangjiakou 
      075000, China.
FAU - Chang, Xiaotong
AU  - Chang X
AD  - Key Laboratory of Clinical Diagnostics, Hebei North University, Zhangjiakou 
      075000, China. *Corresponding author, E-mail: changxt1212@vip. Sina.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
SB  - IM
MH  - Animals
MH  - *Insulin Resistance/genetics
MH  - *Macrophages/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - *Toll-Like Receptor 2/genetics
EDAT- 2019/10/23 06:00
MHDA- 2019/11/05 06:00
CRDT- 2019/10/23 06:00
PHST- 2019/10/23 06:00 [entrez]
PHST- 2019/10/23 06:00 [pubmed]
PHST- 2019/11/05 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2019 Aug;35(8):689-694.