PMID- 31640438
OWN - NLM
STAT- MEDLINE
DCOM- 20201130
LR  - 20201130
IS  - 1940-2465 (Electronic)
IS  - 1066-8969 (Linking)
VI  - 28
IP  - 3
DP  - 2020 May
TI  - The Utility of ERBB4 and RB1 Immunohistochemistry in Distinguishing Chromophobe 
      Renal Cell Carcinoma From Renal Oncocytoma.
PG  - 259-264
LID - 10.1177/1066896919883016 [doi]
AB  - Objectives. Differentiating renal oncocytoma (RO) from chromophobe renal cell 
      carcinoma (ChRCC) can occasionally be challenging. We evaluated the expression of 
      RB1 and ERBB4 in RO and ChRCC, and compared the immunohistochemistry (IHC) 
      results to RB1 and ERBB4 gene abnormalities detected by fluorescence in situ 
      hybridization (FISH). Materials and Methods. Fifty-three kidney resections 
      (ChRCC, n=28; RO, n=25) were stained for RB1 and ERBB4 IHC and FISH was performed 
      to evaluate gene copy number analysis. Results. A loss of RB1 staining was 
      identified in 64% (18/28) of ChRCCs, which was not found in any ROs (0/25; P 
      <.001). FISH analysis revealed 36% (10/28) of ChRCCs contained a RB1 hemizygous 
      deletion with a concordance of 56% (10/18) between the IHC and FISH findings. No 
      RB1 gene copy number variations were detected in any of the ROs (0/25; P <.001) 
      and retained expression of RB1 by IHC. ERBB4 showed cytoplasmic/membranous 
      staining in all ROs and ChRCCs. However, 75% (21/28) of ChRCCs also contained 
      nuclear positivity for ERBB4, which was uncommonly seen in ROs (3/25, 12%; P < 
      .001). A hemizygous ERBB4 gene deletion was detected in 46% of ChRCCs (13/28), 
      but none of the ROs (0/25; 0%). Loss of labeling by RB1 or nuclear staining for 
      ERBB4 IHC identified 25 of 28 (89%) of ChRCCs. Conclusion. In summary, the loss 
      of RB1 expression is a highly specific diagnostic biomarker in distinguishing 
      ChRCC from RO. Nuclear ERBB4 expression also appears to be a sensitive diagnostic 
      biomarker for ChRCC, albeit the mechanism is unknown.
FAU - Sun, Tong
AU  - Sun T
AD  - University of Massachusetts Medical School, UMass Memorial Medical Center, 
      Worcester, MA, USA.
FAU - Hutchinson, Lloyd
AU  - Hutchinson L
AD  - University of Massachusetts Medical School, UMass Memorial Medical Center, 
      Worcester, MA, USA.
FAU - Zhou, Amy G
AU  - Zhou AG
AD  - University of Massachusetts Medical School, UMass Memorial Medical Center, 
      Worcester, MA, USA.
AD  - Johns Hopkins University, Baltimore, MD, USA.
FAU - Liu, Qingqing
AU  - Liu Q
AD  - University of Massachusetts Medical School, UMass Memorial Medical Center, 
      Worcester, MA, USA.
AD  - Icahn School of Medicine at Mount Sinai, New York, NY, USA.
FAU - Cosar, Ediz F
AU  - Cosar EF
AD  - University of Massachusetts Medical School, UMass Memorial Medical Center, 
      Worcester, MA, USA.
FAU - St Cyr, Maryann
AU  - St Cyr M
AD  - University of Massachusetts Medical School, UMass Memorial Medical Center, 
      Worcester, MA, USA.
FAU - Ninteau, Nicole
AU  - Ninteau N
AD  - University of Massachusetts Medical School, UMass Memorial Medical Center, 
      Worcester, MA, USA.
FAU - Dresser, Karen
AU  - Dresser K
AD  - University of Massachusetts Medical School, UMass Memorial Medical Center, 
      Worcester, MA, USA.
FAU - Cheng, Liang
AU  - Cheng L
AD  - Indiana University, Indianapolis, IN, USA.
FAU - Jiang, Zhong
AU  - Jiang Z
AD  - University of Massachusetts Medical School, UMass Memorial Medical Center, 
      Worcester, MA, USA.
FAU - Cornejo, Kristine M
AU  - Cornejo KM
AUID- ORCID: 0000-0002-9490-8583
AD  - University of Massachusetts Medical School, UMass Memorial Medical Center, 
      Worcester, MA, USA.
AD  - Massachusetts General Hospital, Boston, MA, USA.
LA  - eng
PT  - Journal Article
DEP - 20191022
PL  - United States
TA  - Int J Surg Pathol
JT  - International journal of surgical pathology
JID - 9314927
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (RB1 protein, human)
RN  - 0 (Retinoblastoma Binding Proteins)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 2.7.10.1 (ERBB4 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-4)
RN  - Oncocytoma, renal
SB  - IM
MH  - Adenoma, Oxyphilic/*diagnosis
MH  - Biomarkers, Tumor/*analysis
MH  - Carcinoma, Renal Cell/*diagnosis
MH  - Diagnosis, Differential
MH  - Humans
MH  - Immunohistochemistry
MH  - Kidney Neoplasms/*diagnosis
MH  - Receptor, ErbB-4/analysis/*biosynthesis
MH  - Retinoblastoma Binding Proteins/analysis/*biosynthesis
MH  - Ubiquitin-Protein Ligases/analysis/*biosynthesis
OTO - NOTNLM
OT  - ERBB4
OT  - RB1
OT  - chromophobe renal cell carcinoma
OT  - fluorescence in situ hybridization
OT  - immunohistochemistry
OT  - renal oncocytoma
EDAT- 2019/10/24 06:00
MHDA- 2020/12/01 06:00
CRDT- 2019/10/24 06:00
PHST- 2019/10/24 06:00 [pubmed]
PHST- 2020/12/01 06:00 [medline]
PHST- 2019/10/24 06:00 [entrez]
AID - 10.1177/1066896919883016 [doi]
PST - ppublish
SO  - Int J Surg Pathol. 2020 May;28(3):259-264. doi: 10.1177/1066896919883016. Epub 
      2019 Oct 22.