PMID- 31642260
OWN - NLM
STAT- MEDLINE
DCOM- 20191025
LR  - 20200108
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 37
IP  - 8
DP  - 2019 Aug 8
TI  - [Identification of speckle type BTB/POZ protein mutation regulated key metabolic 
      pathways by cell based proteomics and metabolomics].
PG  - 887-896
LID - 10.3724/SP.J.1123.2018.07008 [doi]
AB  - Speckle type BTB/POZ protein (SPOP) is one of the most frequently mutated protein 
      in prostate cancer. In this study, proteomics and metabolomics were integrated to 
      study the effects of SPOP mutation on metabolism. First, LNCaP control (CON), 
      SPOP wild-type (SPOP_WT), and SPOP mutation (SPOP_Y87N and SPOP_F133L) cells were 
      subjected to a metabolomics study. The metabolomics data of LNCaP CON, SPOP_WT, 
      SPOP_Y87N, and SPOP_F133L cells were evaluated by partial least 
      squares-discriminant analysis (PLS-DA). Four groups could be clearly 
      differentiated with an explanation ability of R(2)X=0.512, R(2)Y=0.616 and 
      predictive ability of Q(2)=0.475. Totally, 36 differential metabolites were 
      defined with variable importance for the projection (VIP) value > 1. Then, the 36 
      metabolites were subjected to one-way ANOVA analysis. Fumaric acid, malic acid, 
      citric acid, aspartic acid, and asparagine were increased in LNCaP SPOP mutation 
      cells compared to that in LNCaP SPOP_WT cells. Using a proteomics study, 909 
      differential proteins were found in LNCaP SPOP_Y87N and SPOP_F133L cells. 
      MetaboAnalyst 3.0 was used to enrich metabolic pathways by using differential 
      metabolites. KOBAS 3.0 was used to enrich metabolic pathways by using 
      differential proteins. Both metabolomics and proteomics analysis showed that the 
      tricarboxylic acid (TCA) cycle and aminoacyl-tRNA biosynthesis were significantly 
      changed. To validate these findings, gas chromatography-mass spectrometry 
      (GC-MS)-based metabolomics was performed in Du145 SPOP knock-out cells. The 
      results indicated that the TCA cycle was activated in Du145 SPOP knock-out cells. 
      Collectively, this study found that SPOP mutation significantly promoted TCA 
      cycle in prostate cancer cells.
FAU - Yan, Min
AU  - Yan M
AD  - Chinese Academy of Sciecne Key Laboratory of Separation Sciences for Analytical 
      Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 
      Dalian 116023, China.
AD  - University of Chinese Academy of Sciences, Beijing 100049, China.
FAU - Liu, Jing
AU  - Liu J
AD  - Chinese Academy of Sciecne Key Laboratory of Separation Sciences for Analytical 
      Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 
      Dalian 116023, China.
FAU - Xia, Tian
AU  - Xia T
AD  - Chinese Academy of Sciecne Key Laboratory of Separation Sciences for Analytical 
      Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 
      Dalian 116023, China.
FAU - Xu, Guowang
AU  - Xu G
AD  - Chinese Academy of Sciecne Key Laboratory of Separation Sciences for Analytical 
      Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 
      Dalian 116023, China.
FAU - Piao, Hailong
AU  - Piao H
AD  - Chinese Academy of Sciecne Key Laboratory of Separation Sciences for Analytical 
      Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 
      Dalian 116023, China.
AD  - University of Chinese Academy of Sciences, Beijing 100049, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Nuclear Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (SPOP protein, human)
SB  - IM
MH  - *BTB-POZ Domain
MH  - Cell Line, Tumor
MH  - Citric Acid Cycle
MH  - Humans
MH  - Male
MH  - Metabolic Networks and Pathways
MH  - *Metabolomics
MH  - Mutation
MH  - Nuclear Proteins/*genetics
MH  - Prostatic Neoplasms/*genetics
MH  - *Proteomics
MH  - Repressor Proteins/*genetics
OTO - NOTNLM
OT  - metabolomics
OT  - prostate cancer
OT  - proteomics
OT  - speckle type BTB/POZ protein (SPOP) mutation
EDAT- 2019/10/24 06:00
MHDA- 2019/10/28 06:00
CRDT- 2019/10/24 06:00
PHST- 2019/10/24 06:00 [entrez]
PHST- 2019/10/24 06:00 [pubmed]
PHST- 2019/10/28 06:00 [medline]
AID - 10.3724/SP.J.1123.2018.07008 [doi]
PST - ppublish
SO  - Se Pu. 2019 Aug 8;37(8):887-896. doi: 10.3724/SP.J.1123.2018.07008.