PMID- 31642268
OWN - NLM
STAT- MEDLINE
DCOM- 20191028
LR  - 20200108
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 37
IP  - 11
DP  - 2019 Nov 8
TI  - [Analysis of monosaccharide composition in polysaccharides from Lycium barbarum 
      L. by precolumn labeling under mild conditions and high-performance liquid 
      chromatography coupled with mass spectrometry].
PG  - 1162-1172
LID - 10.3724/SP.J.1123.2019.04013 [doi]
AB  - A method for the separation, identification, and determination of fructose and 
      various aldehyde monosaccharides was established by precolumn labeling with 
      1-phenyl-3-methyl-5-pyrazolone (PMP) and high-performance liquid chromatography 
      coupled with mass spectrometry (HPLC-MS). The separation was performed on a 
      Kromasil-C18 column (100 mmx4.6 mm, 3.5 mum) with gradient elution. The detection 
      was performed by electrospray ionization mass spectrometry in selected ion 
      monitoring (SIM) mode. In this study, the derivatization mechanism of PMP-labeled 
      fructose was proposed under mild NH(3).H(2)O conditions. The suggested method 
      showed good linearity in the corresponding mass concentration ranges, with the 
      correlation coefficients (r(2)) > 0.9947. The limits of detection (LODs) and 
      limits of quantification (LOQs) were in the ranges 0.003 to 0.05 mg/L and 0.01 to 
      0.15 mg/L, respectively. The recoveries in spiked Lycium barbarum L. samples were 
      65.1% to 116.2%, with relative standard deviations (RSDs) of less than 10.2%. By 
      virtue of its simplicity, high sensitivity, and good repeatability, the method 
      could be successfully applied to the analysis of the monosaccharide composition 
      in polysaccharides of Lycium barbarum L. from four planting areas. Results showed 
      that the isolated polysaccharides comprise mannose, fructose, rhamnose, 
      galactose, glucose, xylose, arabinose, and ribose. The concentration distribution 
      of various monosaccharides differed notably depending on the planted 
      environmention. The proposed method is expected to be of great significance in 
      standardizing the quality control of polysaccharides.
FAU - Zhao, Mengxin
AU  - Zhao M
AD  - State Key Laboratory of High-Efficiency Utilization of Coal and Green Chemical 
      Engineering, School of Chemistry and Chemical Engineering, Ningxia University, 
      Yinchuan 750021, China.
FAU - Wang, Zelan
AU  - Wang Z
AD  - State Key Laboratory of High-Efficiency Utilization of Coal and Green Chemical 
      Engineering, School of Chemistry and Chemical Engineering, Ningxia University, 
      Yinchuan 750021, China.
FAU - Meng, Zhe
AU  - Meng Z
AD  - State Key Laboratory of High-Efficiency Utilization of Coal and Green Chemical 
      Engineering, School of Chemistry and Chemical Engineering, Ningxia University, 
      Yinchuan 750021, China.
FAU - Li, Jiguang
AU  - Li J
AD  - State Key Laboratory of High-Efficiency Utilization of Coal and Green Chemical 
      Engineering, School of Chemistry and Chemical Engineering, Ningxia University, 
      Yinchuan 750021, China.
FAU - Li, Heping
AU  - Li H
AD  - State Key Laboratory of High-Efficiency Utilization of Coal and Green Chemical 
      Engineering, School of Chemistry and Chemical Engineering, Ningxia University, 
      Yinchuan 750021, China.
FAU - Liu, Wanyi
AU  - Liu W
AD  - State Key Laboratory of High-Efficiency Utilization of Coal and Green Chemical 
      Engineering, School of Chemistry and Chemical Engineering, Ningxia University, 
      Yinchuan 750021, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Monosaccharides)
RN  - 0 (Phytochemicals)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Lycium/*chemistry
MH  - Monosaccharides/*analysis
MH  - Phytochemicals/analysis
MH  - Polysaccharides/*analysis
MH  - Spectrometry, Mass, Electrospray Ionization
OTO - NOTNLM
OT  - Lycium barbarum L.
OT  - high-performance liquid chromatography-mass spectrometry (HPLC-MS)
OT  - monosaccharide composition
OT  - polysaccharide
OT  - precolumn labeling
EDAT- 2019/10/24 06:00
MHDA- 2019/10/29 06:00
CRDT- 2019/10/24 06:00
PHST- 2019/10/24 06:00 [entrez]
PHST- 2019/10/24 06:00 [pubmed]
PHST- 2019/10/29 06:00 [medline]
AID - 10.3724/SP.J.1123.2019.04013 [doi]
PST - ppublish
SO  - Se Pu. 2019 Nov 8;37(11):1162-1172. doi: 10.3724/SP.J.1123.2019.04013.