PMID- 31642289
OWN - NLM
STAT- MEDLINE
DCOM- 20191025
LR  - 20200108
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 37
IP  - 10
DP  - 2019 Oct 8
TI  - [Determination of 11 sulfonamides in pork by two-step liquid-liquid 
      extraction-solid phase extraction purification coupled with high performance 
      liquid chromatography-tandem mass spectrometry].
PG  - 1098-1104
LID - 10.3724/SP.J.1123.2019.04005 [doi]
AB  - A method based on two-step liquid-liquid extraction-solid phase extraction 
      purification coupled with high performance liquid chromatography-tandem mass 
      spectrometry (LC-MS/MS) was developed for the determination of 11 common 
      sulfonamides in pork. The samples were extracted first with ethyl acetate 
      containing 2%(v/v) formic acid and then with acetone for the second step. The 
      supernatant was degreased with n-hexane and cleaned using an Oasis MCX SPE 
      column. Finally, nitrogen was blown over the obtained mixture, and the mixture 
      was filtered for conducting HPLC-MS/MS analysis. The sulfonamides were detected 
      in the multiple reaction monitoring positive ion mode, and quantification was 
      performed by employing the external standard method with matrix calibration 
      curves. All the 11 sulfonamides demonstrated good linearity in the range of 
      20-400 mug/L, with the correlation coefficient (r(2)) higher than 0.99. The limits 
      of detection (LODs; S/N=3) and limits of quantification (LOQs; S/N=10) were found 
      to be in the ranges of 0.1-1.0 mug/kg and 0.2-3.0 mug/kg, respectively. 
      Furthermore, the results indicated that the average recoveries of the 11 
      sulfonamides ranged from 79.3% to 105.5% at three spiked levels of 50, 100, and 
      200 mug/kg, with the relative standard deviations (RSDs) in the range of 
      1.3%-11.6% (n=6). This method showed higher extraction efficiency than the 
      one-step liquid-liquid extraction, and the detection sensitivity was highly 
      improved.
FAU - Liu, Peiyong
AU  - Liu P
AD  - Dazhou Food and Drug Inspection Institute, Dazhou 635000, China.
FAU - Zhang, Hui
AU  - Zhang H
AD  - Dazhou Food and Drug Inspection Institute, Dazhou 635000, China.
FAU - Mi, Zhijin
AU  - Mi Z
AD  - Dazhou Food and Drug Inspection Institute, Dazhou 635000, China.
FAU - Zhang, Liangcheng
AU  - Zhang L
AD  - Dazhou Food and Drug Inspection Institute, Dazhou 635000, China.
FAU - Zhang, Guangren
AU  - Zhang G
AD  - Dazhou Food and Drug Inspection Institute, Dazhou 635000, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Sulfonamides)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Food Contamination/*analysis
MH  - Liquid-Liquid Extraction
MH  - Red Meat/*analysis
MH  - Solid Phase Extraction
MH  - Sulfonamides/*analysis
MH  - Swine
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)
OT  - pork
OT  - solid phase extraction (SPE)
OT  - sulfonamides
OT  - two-step liquid-liquid extraction
OT  - veterinary drug residues
EDAT- 2019/10/24 06:00
MHDA- 2019/10/28 06:00
CRDT- 2019/10/24 06:00
PHST- 2019/10/24 06:00 [entrez]
PHST- 2019/10/24 06:00 [pubmed]
PHST- 2019/10/28 06:00 [medline]
AID - 10.3724/SP.J.1123.2019.04005 [doi]
PST - ppublish
SO  - Se Pu. 2019 Oct 8;37(10):1098-1104. doi: 10.3724/SP.J.1123.2019.04005.