PMID- 31642300
OWN - NLM
STAT- MEDLINE
DCOM- 20191025
LR  - 20200108
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 37
IP  - 9
DP  - 2019 Sep 8
TI  - [Rapid determination of bongkrekic acid in Liushenqu by ultra performance liquid 
      chromatography-tandem mass spectrometry].
PG  - 963-968
LID - 10.3724/SP.J.1123.2019.03014 [doi]
AB  - A method for the rapid determination of biotoxin (bongkrekic acid) in the 
      Liushenqu was established using ultra performance liquid chromatography-tandem 
      mass spectrometry (UPLC-MS/MS). The samples were extracted with methanol by 
      ultrasonication, then the pH was adjusted to 8 using ammonia. After filtration, 
      the extract was purified using an Oasis Max strong anion exchange resin column. 
      The Waters HSS T3 column (100 mmx2.1 mm, 1.8 mum) was used for the UPLC-MS/MS 
      analysis. The mobile phase was acetonitrile-10 mmol/L ammonium formate solution 
      (containing 0.1% (v/v) formic acid). MS analysis was performed using an 
      electrospray ionization (ESI) source in the negative and multiple reaction 
      monitoring (MRM) modes. Under the optimum conditions, the linear range of 
      bongkrekic acid was 0.5-100 mug/L (correlations coefficient (R(2))>0.99). The 
      recoveries of the bongkrekic acid were 80.6%-85.3%. The intra-day and inter-day 
      relative standard deviations (RSDs) were in the range of 4.2%-6.8% and 
      8.2%-13.2%, respectively. The limit of detection (LOD) and limit of 
      quantification (LOQ) were 0.4 mug/kg and 1.2 mug/kg, respectively. The results 
      showed that bongkrekic acid residues were detected in Liushenqu, thereby 
      confirming the supporting role of our method for the risk monitoring of biotoxins 
      in health foods and Chinese herbal medicines. This method is simple, easy, 
      sensitive, and suitable for the determination of the bongkrekic acid residues in 
      Liushenqu.
FAU - Wang, Junhu
AU  - Wang J
AD  - Taizhou Institute for Food and Drug Control, Taizhou 225300, China.
FAU - Qiao, Yongsheng
AU  - Qiao Y
AD  - Taizhou Institute for Food and Drug Control, Taizhou 225300, China.
FAU - Wang, Jian
AU  - Wang J
AD  - Taizhou Institute for Food and Drug Control, Taizhou 225300, China.
FAU - Zhu, Yuan
AU  - Zhu Y
AD  - Taizhou Institute for Food and Drug Control, Taizhou 225300, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Drugs, Chinese Herbal)
RN  - 11076-19-0 (Bongkrekic Acid)
SB  - IM
MH  - Bongkrekic Acid/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - *Drug Contamination
MH  - Drugs, Chinese Herbal/*analysis
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Liushenqu
OT  - biotoxin
OT  - bongkrekic acid
OT  - ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)
EDAT- 2019/10/24 06:00
MHDA- 2019/10/28 06:00
CRDT- 2019/10/24 06:00
PHST- 2019/10/24 06:00 [entrez]
PHST- 2019/10/24 06:00 [pubmed]
PHST- 2019/10/28 06:00 [medline]
AID - 10.3724/SP.J.1123.2019.03014 [doi]
PST - ppublish
SO  - Se Pu. 2019 Sep 8;37(9):963-968. doi: 10.3724/SP.J.1123.2019.03014.