PMID- 31642841
OWN - NLM
STAT- MEDLINE
DCOM- 20200908
LR  - 20200908
IS  - 2050-7518 (Electronic)
IS  - 2050-750X (Linking)
VI  - 7
IP  - 41
DP  - 2019 Oct 23
TI  - An immunochromatographic strip sensor for sildenafil and its analogues.
PG  - 6383-6389
LID - 10.1039/c9tb00280d [doi]
AB  - In this study, a new hapten of sildenafil (Sild) was successfully synthesized and 
      a sensitive monoclonal antibody (mAb) against Sild was produced based on this new 
      hapten. The subclass of the mAb was IgG2a, and the half-maximal inhibitory 
      concentration (IC50) of the mAb was 0.53 ng mL-1. Next, an immunochromatographic 
      assay (ICA) was established for detecting Sild and its analogues in functional 
      foods, where the visual detection limit (vLOD) and cut-off values were 0.5 and 20 
      mug kg-1, respectively. With the aid of a strip scan reader, the ICA can measure 
      Sild with an LOD of 0.7 mug kg-1 and a line range of detection between 1.40 and 
      13.11 mug kg-1. The whole test process takes only 15 min. Therefore, the ICA 
      provides a useful tool for the on-site detection and rapid initial screening of 
      Sild in functional food samples.
FAU - Wang, Zhongxing
AU  - Wang Z
AD  - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 
      People's Republic of China. kuangh@jiangnan.edu.cn xcl@jiangnan.edu.cn and 
      International Joint Research Laboratory for Biointerface and Biodetection, and 
      School of Food Science and Technology, Jiangnan University, Wuxi, People's 
      Republic of China.
FAU - Wu, Xiaoling
AU  - Wu X
AD  - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 
      People's Republic of China. kuangh@jiangnan.edu.cn xcl@jiangnan.edu.cn and 
      International Joint Research Laboratory for Biointerface and Biodetection, and 
      School of Food Science and Technology, Jiangnan University, Wuxi, People's 
      Republic of China.
FAU - Liu, Liqiang
AU  - Liu L
AD  - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 
      People's Republic of China. kuangh@jiangnan.edu.cn xcl@jiangnan.edu.cn and 
      International Joint Research Laboratory for Biointerface and Biodetection, and 
      School of Food Science and Technology, Jiangnan University, Wuxi, People's 
      Republic of China.
FAU - Xu, Liguang
AU  - Xu L
AD  - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 
      People's Republic of China. kuangh@jiangnan.edu.cn xcl@jiangnan.edu.cn and 
      International Joint Research Laboratory for Biointerface and Biodetection, and 
      School of Food Science and Technology, Jiangnan University, Wuxi, People's 
      Republic of China.
FAU - Kuang, Hua
AU  - Kuang H
AUID- ORCID: 0000-0002-2724-8722
AD  - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 
      People's Republic of China. kuangh@jiangnan.edu.cn xcl@jiangnan.edu.cn and 
      International Joint Research Laboratory for Biointerface and Biodetection, and 
      School of Food Science and Technology, Jiangnan University, Wuxi, People's 
      Republic of China.
FAU - Xu, Chuanlai
AU  - Xu C
AUID- ORCID: 0000-0002-5639-7102
AD  - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 
      People's Republic of China. kuangh@jiangnan.edu.cn xcl@jiangnan.edu.cn and 
      International Joint Research Laboratory for Biointerface and Biodetection, and 
      School of Food Science and Technology, Jiangnan University, Wuxi, People's 
      Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Mater Chem B
JT  - Journal of materials chemistry. B
JID - 101598493
RN  - 0 (Antibodies, Monoclonal)
RN  - BW9B0ZE037 (Sildenafil Citrate)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Chromatography, Affinity/*methods
MH  - Food Contamination/analysis
MH  - Immunoassay/methods
MH  - Limit of Detection
MH  - Sildenafil Citrate/analogs & derivatives/*analysis
EDAT- 2019/10/24 06:00
MHDA- 2020/09/09 06:00
CRDT- 2019/10/24 06:00
PHST- 2019/10/24 06:00 [entrez]
PHST- 2019/10/24 06:00 [pubmed]
PHST- 2020/09/09 06:00 [medline]
AID - 10.1039/c9tb00280d [doi]
PST - ppublish
SO  - J Mater Chem B. 2019 Oct 23;7(41):6383-6389. doi: 10.1039/c9tb00280d.