PMID- 31645629
OWN - NLM
STAT- MEDLINE
DCOM- 20201029
LR  - 20210110
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Oct 23
TI  - Antigen array for serological diagnosis and novel allergen identification in 
      severe equine asthma.
PG  - 15170
LID - 10.1038/s41598-019-51820-7 [doi]
LID - 15170
AB  - Severe equine asthma (sEA), which closely resembles human asthma, is a 
      debilitating and performance-limiting allergic respiratory disorder which affects 
      14% of horses in the Northern Hemisphere and is associated with increased 
      allergen-specific immunoglobulin E (IgE) against a range of environmental 
      proteins. A comprehensive microarray platform was developed to enable the 
      simultaneous detection of allergen-specific equine IgE in serum against a wide 
      range of putative allergenic proteins. The microarray revealed a plethora of 
      novel pollen, bacteria, mould and arthropod proteins significant in the aetiology 
      of sEA. Moreover, the analyses revealed an association between sEA-affected 
      horses and IgE antibodies specific for proteins derived from latex, which has 
      traditionally been ubiquitous to the horse's environment in the form of riding 
      surfaces and race tracks. Further work is required to establish the involvement 
      of latex proteins in sEA as a potential risk factor. This work demonstrates a 
      novel and rapid approach to sEA diagnosis, providing a platform for tailored 
      management and the development of allergen-specific immunotherapy.
FAU - White, S J
AU  - White SJ
AUID- ORCID: 0000-0002-3675-7545
AD  - Royal Agricultural University, Cirencester, Gloucestershire, GL7 6JS, UK. 
      samuel.white@ntu.ac.uk.
AD  - School of Biosciences, University of Nottingham, Sutton Bonington Campus, 
      Loughborough, LE12 5RD, UK. samuel.white@ntu.ac.uk.
AD  - Nottingham Trent University, Brackenhurst Campus, Southwell, Nottinghamshire, 
      NG25 0QF, UK. samuel.white@ntu.ac.uk.
FAU - Moore-Colyer, M
AU  - Moore-Colyer M
AD  - Royal Agricultural University, Cirencester, Gloucestershire, GL7 6JS, UK.
FAU - Marti, E
AU  - Marti E
AD  - Department of Clinical Research and Veterinary Public Health, University of Bern, 
      Bremgartenstr, Postfach, 3001, Bern, Switzerland.
FAU - Hannant, D
AU  - Hannant D
AUID- ORCID: 0000-0003-0792-7418
AD  - School of Veterinary Medicine and Science, University of Nottingham, Sutton 
      Bonington Campus, Loughborough, LE12 5RD, UK.
FAU - Gerber, V
AU  - Gerber V
AD  - Department of Clinical Research and Veterinary Public Health, University of Bern, 
      Bremgartenstr, Postfach, 3001, Bern, Switzerland.
FAU - Couetil, L
AU  - Couetil L
AUID- ORCID: 0000-0002-1516-6666
AD  - Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, 
      West Lafayette, IN, 47907, USA.
FAU - Richard, E A
AU  - Richard EA
AUID- ORCID: 0000-0002-5890-8658
AD  - LABEO Frank Duncombe, 1 route de Rosel, 14053, Caen, Cedex 4, France.
AD  - Normandie Univ, UniCaen, BIOTARGEN, 3 rue Nelson Mandela, 14280, Saint-Contest, 
      France.
FAU - Alcocer, M
AU  - Alcocer M
AD  - School of Biosciences, University of Nottingham, Sutton Bonington Campus, 
      Loughborough, LE12 5RD, UK.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191023
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Allergens)
RN  - 0 (Antigens)
SB  - IM
CIN - Equine Vet J. 2020 Jan;52(1):11-12. PMID: 31821611
MH  - Allergens/*blood
MH  - Animals
MH  - Antigens/*blood
MH  - Asthma/blood/*diagnosis/*veterinary
MH  - Discriminant Analysis
MH  - Horses/*blood/*immunology
MH  - Least-Squares Analysis
MH  - *Serologic Tests
PMC - PMC6811683
COIS- The authors declare no competing interests.
EDAT- 2019/10/28 06:00
MHDA- 2020/10/30 06:00
PMCR- 2019/10/23
CRDT- 2019/10/25 06:00
PHST- 2019/04/24 00:00 [received]
PHST- 2019/10/08 00:00 [accepted]
PHST- 2019/10/25 06:00 [entrez]
PHST- 2019/10/28 06:00 [pubmed]
PHST- 2020/10/30 06:00 [medline]
PHST- 2019/10/23 00:00 [pmc-release]
AID - 10.1038/s41598-019-51820-7 [pii]
AID - 51820 [pii]
AID - 10.1038/s41598-019-51820-7 [doi]
PST - epublish
SO  - Sci Rep. 2019 Oct 23;9(1):15170. doi: 10.1038/s41598-019-51820-7.