PMID- 31646361
OWN - NLM
STAT- MEDLINE
DCOM- 20200311
LR  - 20200311
IS  - 1432-0703 (Electronic)
IS  - 0090-4341 (Linking)
VI  - 78
IP  - 1
DP  - 2020 Jan
TI  - Assessment of Gene Expression Biomarkers in the Chilean Pencil Catfish, 
      Trichomycterus areolatus, from the Choapa River Basin, Coquimbo Chile.
PG  - 137-148
LID - 10.1007/s00244-019-00678-x [doi]
AB  - The objective of this study was to describe changes in the gene expression in the 
      Chilean catfish, Trichomycterus areolatus, based on their geographic location 
      within the Choapa River. Genes of choice included those that are biomarkers of 
      exposure to metals, oxidative stress, and endocrine disruption. Male and female 
      T. areolatus were sampled from four sites in January 2015 differently impacted by 
      human activities. In males, but not females, hepatic gene expression of heat 
      shock protein (HSP70) and cytochrome P450 1A (CYP1A) were significantly elevated 
      at the site adjacent to the small city of Salamanca, relative to the other sites. 
      In females, hepatic HSP70, the aryl hydrocarbon receptor (AHR), and the estrogen 
      responsive genes, vitellogenin (VTG) and estrogen receptor alpha (ERalpha), were 
      significantly lower at the site located furthest downstream. A similar downstream 
      pattern of lower expression levels also was found in ovarian tissue for the 
      genes, HSP70 and ERalpha. Gill gene expression showed a unique pattern in females as 
      levels of metallothionein were elevated at the site furthest downstream. While 
      analytical chemistry of water samples provided limited evidence of agrichemical 
      contamination, the gene expression data are consistent with an exposure to 
      agrichemicals and metals. T. areolatus may be a valuable sentinel organism and 
      its use as a bioindicator species in some rivers within Chile can provide 
      considerable insight, particularly in situations analytical chemistry is limited 
      by environmental constraints.
FAU - Ali, Jonathan M
AU  - Ali JM
AD  - Department of Environmental, Agricultural and Occupational Health, University of 
      Nebraska - Medical Center, Omaha, NE, 68198-6805, USA.
FAU - Montecinos, Angela
AU  - Montecinos A
AD  - Departamento de Biologia, Universidad de La Serena, Raul Bitran 1305, La Serena, 
      Chile.
FAU - Schulze, Thomas T
AU  - Schulze TT
AD  - Department of Biology, University of Nebraska at Omaha, Omaha, NE, 68182-0040, 
      USA.
FAU - Allmon, Luke G
AU  - Allmon LG
AD  - Department of Biology, University of Nebraska at Omaha, Omaha, NE, 68182-0040, 
      USA.
FAU - Kallenbach, Alex T
AU  - Kallenbach AT
AD  - Department of Biology, University of Nebraska at Omaha, Omaha, NE, 68182-0040, 
      USA.
FAU - Watson, Gabrielle F
AU  - Watson GF
AD  - Department of Biology, University of Nebraska at Omaha, Omaha, NE, 68182-0040, 
      USA.
FAU - Davis, Paul H
AU  - Davis PH
AD  - Department of Biology, University of Nebraska at Omaha, Omaha, NE, 68182-0040, 
      USA.
FAU - Snow, Daniel D
AU  - Snow DD
AD  - School of Natural Resources, University of Nebraska-Lincoln, Lincoln, NE, 
      68583-0844, USA.
FAU - Bertin, Angeline
AU  - Bertin A
AD  - Departamento de Biologia, Universidad de La Serena, Raul Bitran 1305, La Serena, 
      Chile.
FAU - Gouin, Nicolas
AU  - Gouin N
AD  - Departamento de Biologia, Universidad de La Serena, Raul Bitran 1305, La Serena, 
      Chile.
AD  - Centro de Estudios Avanzados en Zonas Aridas (CEAZA), Raul Bitran 1305, La 
      Serena, Chile.
AD  - Instituto de Investigacion Multidisciplinar en Ciencia y Tecnologia, Universidad 
      de La Serena, La Serena, Chile.
FAU - Kolok, Alan S
AU  - Kolok AS
AD  - Idaho Water Resources Research Institute, University of Idaho, 875 Perimeter 
      Drive, MS 3002, Moscow, ID, 83844-3002, USA. akolok@uidaho.edu.
LA  - eng
PT  - Journal Article
DEP - 20191023
PL  - United States
TA  - Arch Environ Contam Toxicol
JT  - Archives of environmental contamination and toxicology
JID - 0357245
RN  - 0 (Agrochemicals)
RN  - 0 (Biomarkers)
RN  - 0 (Endocrine Disruptors)
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - Agrochemicals/analysis/toxicity
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Catfishes/*genetics/metabolism
MH  - Chile
MH  - Endocrine Disruptors/analysis/toxicity
MH  - Environmental Monitoring/*methods
MH  - Female
MH  - Gene Expression/*drug effects
MH  - Geologic Sediments/chemistry
MH  - Male
MH  - Oxidative Stress/*drug effects/genetics
MH  - Rivers/*chemistry
MH  - Sex Characteristics
MH  - Water Pollutants, Chemical/analysis/*toxicity
EDAT- 2019/10/28 06:00
MHDA- 2020/03/12 06:00
CRDT- 2019/10/25 06:00
PHST- 2019/06/19 00:00 [received]
PHST- 2019/10/03 00:00 [accepted]
PHST- 2019/10/28 06:00 [pubmed]
PHST- 2020/03/12 06:00 [medline]
PHST- 2019/10/25 06:00 [entrez]
AID - 10.1007/s00244-019-00678-x [pii]
AID - 10.1007/s00244-019-00678-x [doi]
PST - ppublish
SO  - Arch Environ Contam Toxicol. 2020 Jan;78(1):137-148. doi: 
      10.1007/s00244-019-00678-x. Epub 2019 Oct 23.