PMID- 31646573
OWN - NLM
STAT- MEDLINE
DCOM- 20201215
LR  - 20201215
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 19
DP  - 2019 Oct
TI  - MicroRNA-486 inhibits cell proliferation, invasion and migration via 
      down-regulating the TENM1 expressions and affecting ERK and Akt signaling 
      pathways and epithelial-to-mesenchymal transition in papillary thyroid carcinoma.
PG  - 8429-8439
LID - 19155 [pii]
LID - 10.26355/eurrev_201910_19155 [doi]
AB  - OBJECTIVE: Papillary thyroid carcinoma (PTC) is one of the general thyroid 
      malignancies. Recently, microRNAs (miRNAs) have identified as pivotal gene 
      regulators in PTC tumorigenesis. The aim of this study was to investigate the 
      role of miR-486 in PTC and its underlying mechanism. PATIENTS AND METHODS: 
      Fifty-six pairs of PTC tissue and matched normal tissue samples were collected 
      from PTC patients who underwent surgery at our hospital from March 2015 to 
      September 2017. Human thyroid epithelial cell line Nthy-ori3-1and PTC cell lines 
      (BCPAP, K1, HTH83, and TPC-1) were cultured. The mRNA and protein expression 
      level were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) 
      and Western blot, respectively. Additionally, the proliferation and migration 
      abilities were checked by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl 
      tetrazolium bromide) method and transwell assay, respectively. Furthermore, 
      dual-luciferase reporter assay was performed to confirm the combination of 
      miR-486 and TENM1. Xenograft Model experiments were performed to assess the 
      effects of miR-486 on tumor growth in vivo. RESULTS: MiR-486 expression was 
      significantly reduced in PTC, which was associated with the poorer 
      clinicopathologic characteristics and overall survival (OS) of PTC patients. 
      Moreover, miR-486 restoration in PTC cells was confirmed to markedly inhibit 
      proliferation, invasion, and migration via the regulation of 
      extracellular-signal-regulated kinase (ERK) and protein kinase B (Akt) signaling 
      pathways and epithelial-mesenchymal transition (EMT). In the meantime, teneurin 
      transmembrane protein 1 (TENM1) was identified as a direct functional target for 
      miR-486 in PTC cells on the basis of bioinformatic analysis and luciferase 
      reporter assays. Additionally, we also verified that miR-486 restoration could 
      prominently repress the PTC growth in vivo. CONCLUSIONS: MiR-486 exerted 
      anti-tumor functions in PTC progression and served as promising biomarkers for 
      the PTC treatment.
FAU - Sun, Y-H
AU  - Sun YH
AD  - Department of Ultrasound, Yantai Affiliated Hospital of Binzhou Medical 
      University, Yantai, China. bzheggr@163.com.
FAU - Liu, Z-F
AU  - Liu ZF
FAU - Yang, B-B
AU  - Yang BB
FAU - Yu, B
AU  - Yu B
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (MIRN486 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Tenascin)
RN  - 0 (teneurin-1)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Cell Movement
MH  - Cell Proliferation
MH  - *Down-Regulation
MH  - *Epithelial-Mesenchymal Transition/genetics
MH  - Extracellular Signal-Regulated MAP Kinases/*metabolism
MH  - Humans
MH  - MicroRNAs/genetics/*metabolism
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - Signal Transduction
MH  - Tenascin/genetics/*metabolism
MH  - Thyroid Cancer, Papillary/genetics/*metabolism/pathology
MH  - Tumor Cells, Cultured
EDAT- 2019/10/28 06:00
MHDA- 2020/12/16 06:00
CRDT- 2019/10/25 06:00
PHST- 2019/10/25 06:00 [entrez]
PHST- 2019/10/28 06:00 [pubmed]
PHST- 2020/12/16 06:00 [medline]
AID - 19155 [pii]
AID - 10.26355/eurrev_201910_19155 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Oct;23(19):8429-8439. doi: 
      10.26355/eurrev_201910_19155.