PMID- 31646589
OWN - NLM
STAT- MEDLINE
DCOM- 20201215
LR  - 20201215
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 19
DP  - 2019 Oct
TI  - Silence of lncRNA XIST represses myocardial cell apoptosis in rats with acute 
      myocardial infarction through regulating miR-449.
PG  - 8566-8572
LID - 19172 [pii]
LID - 10.26355/eurrev_201910_19172 [doi]
AB  - OBJECTIVE: To study the influences of long non-coding ribonucleic acid (lncRNA) 
      X-inactive specific transcript (XIST) on rats with acute myocardial infarction 
      (AMI), and its regulatory mechanism. MATERIALS AND METHODS: A total of 30 
      Sprague-Dawley rats were randomly assigned into Sham group, Model group, and 
      lncRNA XIST small interfering RNA (XIST siRNA) group. The AMI rat model was 
      prepared through ligating the left anterior descending coronary artery. The left 
      ventricular end-diastolic volume (LVEDV), left ventricular end-systolic volume 
      (LVESV), left ventricular systolic diameter (LVDs), and left ventricular 
      diastolic diameter (LVDd) of rats were determined using a color Doppler 
      ultrasound system. Reverse transcription-polymerase chain reaction was performed 
      to measure the expression levels of lncRNA XIST, microRNA (miR)-449, and Notch1 
      in rat heart tissues in each group. Pathological morphology of rat heart tissues 
      in each group was observed via hematoxylin-eosin (HE) staining. Cell apoptosis in 
      rat heart tissues was evaluated through terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) assay. RESULTS: Compared with 
      those in Sham group, rats in Model group had significantly increased LVEDV, 
      LVESV, LVDs, and LVDd. After transfection with lncRNA XIST siRNA, XIST level in 
      rat heart tissues was remarkably declined in XIST siRNA group compared with that 
      in Model group. According to HE staining results, the pathological injuries in 
      rat heart tissues were greatly improved in XIST siRNA group compared with those 
      in Model group. TUNEL staining results revealed that the apoptosis rate of cells 
      in rat heart tissues in XIST siRNA group was markedly lower than that in Model 
      group. Higher level of miR-449 and lower level of Notch1 were observed in rats of 
      XIST siRNA group than those of Model group. CONCLUSIONS: Knockdown of lncRNA XIST 
      can repress the myocardial cell apoptosis in AMI model rats by downregulating 
      miR-449 level.
FAU - Zhang, M
AU  - Zhang M
AD  - Heart Center, Aerospace Center Hospital, Beijing, China. mengzhangmd@126.com.
FAU - Liu, H-Y
AU  - Liu HY
FAU - Han, Y-L
AU  - Han YL
FAU - Wang, L
AU  - Wang L
FAU - Zhai, D-D
AU  - Zhai DD
FAU - Ma, T
AU  - Ma T
FAU - Zhang, M-J
AU  - Zhang MJ
FAU - Liang, C-Z
AU  - Liang CZ
FAU - Shen, Y
AU  - Shen Y
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (MIRN449 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (XIST non-coding RNA)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - *Apoptosis/genetics
MH  - Disease Models, Animal
MH  - Gene Silencing
MH  - Male
MH  - MicroRNAs/genetics/*metabolism
MH  - Myocardial Infarction/*metabolism/pathology
MH  - Myocytes, Cardiac/*metabolism/pathology
MH  - RNA, Long Noncoding/genetics/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2019/10/28 06:00
MHDA- 2020/12/16 06:00
CRDT- 2019/10/25 06:00
PHST- 2019/10/25 06:00 [entrez]
PHST- 2019/10/28 06:00 [pubmed]
PHST- 2020/12/16 06:00 [medline]
AID - 19172 [pii]
AID - 10.26355/eurrev_201910_19172 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Oct;23(19):8566-8572. doi: 
      10.26355/eurrev_201910_19172.