PMID- 31646794
OWN - NLM
STAT- MEDLINE
DCOM- 20200304
LR  - 20220218
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 24
IP  - 4
DP  - 2019 Jul-Aug
TI  - MicroRNA-24 regulates the growth and chemosensitivity of the human colorectal 
      cancer cells by targeting RNA-binding protein DND1.
PG  - 1476-1481
AB  - PURPOSE: This study was designed to investigate the role and therapeutic 
      potential of miR-24 in colorectal cancer (CRC). METHODS: The CRC cell lines 
      HCT116, RKO, SW480, SW48, and the non-cancer cell line CCD-18Co were used in the 
      present study. The miR-24 expression was determined by qRT-PCR analysis. Cell 
      viability was determined by MTT assay. Apoptosis was examined by acridine orange 
      (AO)/ethidium bromide (EB) and annexin V/propidium iodide (PI) staining. 
      Transfection was performed by Lipofectamine 2000. Protein levels were examined by 
      western blot analysis. RESULTS: miR-24 was significantly downregulated in CRC 
      cell lines. Ectopic expression of miR-24 caused significant decrease in the cell 
      viability by initiating apoptotic cell death of colorectal SW48 cancer cells, 
      indicative of its tumor suppressive role. Moreover, miR-24 overexpression also 
      enhanced the chemosensitivity of SW48 cells to 5-fluorouracil (5-FU). In silico 
      analysis together with dual luciferase reporter assay indicated the RNA binding 
      protein DND1 was the potential target of miR-24 in SW48 cells. Investigation of 
      DND1 expression in CRC cell lines showed up to 5.3-fold upregulation of DND1. 
      Nonetheless, ectopic expression of miR-24 in SW48 cells resulted in the 
      downregulation of DND1 expression. Additionally, silencing of DND1 in the SW48 
      cells also caused inhibition of SW48 cell proliferation. Moreover, overexpression 
      of DND1 could rescue the tumor suppressive effects of miR-24, indicating direct 
      involvement of DND1 in the miR-24 mediated inhibitory effects on SW48 cell 
      proliferation. CONCLUSION: The miR-24 acts as a tumor suppressor and may prove 
      essential in the treatment of CRC.
FAU - Zhang, Qiuhuan
AU  - Zhang Q
AD  - Department of Colorectal and Anal Surgery, The First Affiliated Hospital of 
      Guangxi Medical University, Nanning ，Guangxi 530021, China.
FAU - Li, Wenjing
AU  - Li W
FAU - Liu, Gang
AU  - Liu G
FAU - Tang, Weizhong
AU  - Tang W
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (Dnd1 protein, human)
RN  - 0 (MIRN24 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - U3P01618RT (Fluorouracil)
SB  - IM
RIN - JBUON 2021 November-December; 26(6): 2724
MH  - Apoptosis/drug effects/genetics
MH  - Cell Proliferation/drug effects/*genetics
MH  - Cell Survival/drug effects
MH  - Colorectal Neoplasms/drug therapy/*genetics/pathology
MH  - Drug Resistance, Neoplasm/genetics
MH  - Fluorouracil/pharmacology
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - HCT116 Cells
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Neoplasm Proteins/*genetics
MH  - RNA-Binding Proteins/genetics
EDAT- 2019/10/28 06:00
MHDA- 2020/03/05 06:00
CRDT- 2019/10/25 06:00
PHST- 2019/10/25 06:00 [entrez]
PHST- 2019/10/28 06:00 [pubmed]
PHST- 2020/03/05 06:00 [medline]
PST - ppublish
SO  - J BUON. 2019 Jul-Aug;24(4):1476-1481.