PMID- 31646808
OWN - NLM
STAT- MEDLINE
DCOM- 20200304
LR  - 20211204
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 24
IP  - 4
DP  - 2019 Jul-Aug
TI  - Inhibition of endometrial carcinoma by Kaempferol is interceded through apoptosis 
      induction, G2/M phase cell cycle arrest, suppression of cell invasion and 
      upregulation of m-TOR/PI3K signalling pathway.
PG  - 1555-1561
AB  - PURPOSE: The main purpose of this study was to investigate the selective 
      anticancer effects of Kaempferol against MFE-280 endometrial carcinoma cells 
      along with evaluating its effects on apoptotic pathway, cell cycle phase 
      distribution, cell invasion, cell migration and m-TOR/PI3K/Akt signalling 
      pathway. METHODS: Cell viability of MFE-280 endometrial carcinoma cells was 
      assessed by MTS 
      [(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)] 
      assay. Apoptosis was determined by acridine orange (AO)/ ethidium bromide (EB) 
      double staining. Cell cycle analysis was determined by flow cytometry, while 
      Boyden chamber assay was performed to study the effects of Kaempferol on cell 
      migration and cell invasion, respectively. The effects of Kaempferol on the 
      protein expression of m-TOR/PI3K/Akt signalling pathway were analysed by Western 
      blot assay. RESULTS: Kaempferol exerted considerable and selective anticancer 
      effects on MFE-280 endometrial carcinoma cells with IC50 of 10 muM. The anticancer 
      effects were found to be due to activation of mitochondrial-mediated apoptotic 
      pathway and G2/M phase cell cycle arrest. Furthermore, the results also revealed 
      that Kaempferol significantly inhibited cell migration and cell invasion trend of 
      these cancer cells. Our results also showed that, in comparison to the untreated 
      cells, Kaempferol-treated cells exhibited a dose-dependent downregulation of 
      p-mTOR, p-PI3K and p-AKT proteins. However, mTOR, PI3K and Akt expression levels 
      remained more or less unaltered. CONCLUSIONS: In conclusion, the present study 
      indicates that Kaempferol could exert anticancer effects in MFE-280 endometrial 
      carcinoma cells selectively and that these effects were mediated via apoptosis 
      induction, cell cycle arrest and inhibition of m-TOR/PI3K/Akt signalling pathway.
FAU - Lei, Xia
AU  - Lei X
AD  - Department of Gynaecology, Yanan University Affiliated Hospital, Yanan, Shaanxi , 
      China.
FAU - Guo, Jing
AU  - Guo J
FAU - Wang, Yuan
AU  - Wang Y
FAU - Cui, Jie
AU  - Cui J
FAU - Feng, Bei
AU  - Feng B
FAU - Su, Yani
AU  - Su Y
FAU - Zhao, Hong
AU  - Zhao H
FAU - Yang, Weiwei
AU  - Yang W
FAU - Hu, Yunfeng
AU  - Hu Y
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (Kaempferols)
RN  - 731P2LE49E (kaempferol)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Cell Cycle Checkpoints/drug effects
MH  - Cell Line, Tumor
MH  - Cell Movement/drug effects
MH  - Cell Proliferation/*drug effects
MH  - Endometrial Neoplasms/*drug therapy/genetics/pathology
MH  - Female
MH  - G2 Phase Cell Cycle Checkpoints/drug effects
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Humans
MH  - Kaempferols/*pharmacology
MH  - Neoplasm Invasiveness/*genetics/pathology
MH  - Phosphatidylinositol 3-Kinases/genetics
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/genetics
EDAT- 2019/10/28 06:00
MHDA- 2020/03/05 06:00
CRDT- 2019/10/25 06:00
PHST- 2019/10/25 06:00 [entrez]
PHST- 2019/10/28 06:00 [pubmed]
PHST- 2020/03/05 06:00 [medline]
PST - ppublish
SO  - J BUON. 2019 Jul-Aug;24(4):1555-1561.