PMID- 31647546
OWN - NLM
STAT- MEDLINE
DCOM- 20201001
LR  - 20211204
IS  - 1537-6613 (Electronic)
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 221
IP  - 2
DP  - 2020 Jan 2
TI  - Functional Human CD141+ Dendritic Cells in Human Immune System Mice.
PG  - 201-213
LID - 10.1093/infdis/jiz432 [doi]
AB  - BACKGROUND: For the purpose of studying functional human dendritic cells (DCs) in 
      a humanized mouse model that mimics the human immune system (HIS), a model 
      referred to as HIS mice was established. METHODS: Human immune system mice were 
      made by engrafting NOD/SCID/IL2Rgammanull (NSG) mice with human hematopoietic 
      stem cells (HSCs) following the transduction of genes encoding human cytokines 
      and human leukocyte antigen (HLA)-A2.1 by adeno-associated virus serotype 9 
      (AAV9) vectors. RESULTS: Our results indicate that human DC subsets, such as 
      CD141+CD11c+ and CD1c+CD11c+ myeloid DCs, distribute throughout several organs in 
      HIS mice including blood, bone marrow, spleen, and draining lymph nodes. The 
      CD141+CD11c+ and CD1c+CD11c+ human DCs isolated from HIS mice immunized with 
      adenoviruses expressing malaria/human immunodeficiency virus (HIV) epitopes were 
      able to induce the proliferation of malaria/HIV epitopes-specific human CD8+ T 
      cells in vitro. Upregulation of CD1c was also observed in human CD141+ DCs 1 day 
      after immunization with the adenovirus-based vaccines. CONCLUSIONS: Establishment 
      of such a humanized mouse model that mounts functional human DCs enables 
      preclinical assessment of the immunogenicity of human vaccines in vivo.
CI  - (c) The Author(s) 2019. Published by Oxford University Press for the Infectious 
      Diseases Society of America. All rights reserved. For permissions, e-mail: 
      journals.permissions@oup.com.
FAU - Coelho-Dos-Reis, Jordana G A
AU  - Coelho-Dos-Reis JGA
AD  - Aaron Diamond AIDS Research Center, Affiliate of The Rockefeller University, New 
      York, New York, USA.
AD  - Department of Microbiology, Universidade Federal de Minas Gerais, Minas Gerais, 
      Brazil.
FAU - Funakoshi, Ryota
AU  - Funakoshi R
AD  - Aaron Diamond AIDS Research Center, Affiliate of The Rockefeller University, New 
      York, New York, USA.
FAU - Huang, Jing
AU  - Huang J
AD  - Aaron Diamond AIDS Research Center, Affiliate of The Rockefeller University, New 
      York, New York, USA.
FAU - Pereira, Felipe Valenca
AU  - Pereira FV
AD  - Aaron Diamond AIDS Research Center, Affiliate of The Rockefeller University, New 
      York, New York, USA.
AD  - Federal University of Sao Paulo, Sao Paulo, Brazil.
FAU - Iketani, Sho
AU  - Iketani S
AD  - Aaron Diamond AIDS Research Center, Affiliate of The Rockefeller University, New 
      York, New York, USA.
AD  - Department of Microbiology and Immunology, Columbia University Irving Medical 
      Center, New York, New York, USA.
FAU - Tsuji, Moriya
AU  - Tsuji M
AD  - Aaron Diamond AIDS Research Center, Affiliate of The Rockefeller University, New 
      York, New York, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Adenovirus Vaccines)
RN  - 0 (Antigens, Surface)
RN  - 0 (THBD protein, human)
RN  - 0 (Thrombomodulin)
SB  - IM
MH  - Adenovirus Vaccines/*immunology
MH  - Animals
MH  - Antigens, Surface/*immunology
MH  - Dendritic Cells/*immunology
MH  - Disease Models, Animal
MH  - Humans
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - Thrombomodulin
PMC - PMC7457331
OTO - NOTNLM
OT  - CD141
OT  - CD1c
OT  - adenovirus vaccine
OT  - human dendritic cells
OT  - human immune system mice
EDAT- 2019/10/28 06:00
MHDA- 2020/10/02 06:00
PMCR- 2021/01/02
CRDT- 2019/10/25 06:00
PHST- 2018/12/12 00:00 [received]
PHST- 2019/08/20 00:00 [accepted]
PHST- 2019/10/28 06:00 [pubmed]
PHST- 2020/10/02 06:00 [medline]
PHST- 2019/10/25 06:00 [entrez]
PHST- 2021/01/02 00:00 [pmc-release]
AID - 5606696 [pii]
AID - jiz432 [pii]
AID - 10.1093/infdis/jiz432 [doi]
PST - ppublish
SO  - J Infect Dis. 2020 Jan 2;221(2):201-213. doi: 10.1093/infdis/jiz432.