PMID- 31648045
OWN - NLM
STAT- MEDLINE
DCOM- 20200608
LR  - 20200608
IS  - 1879-3177 (Electronic)
IS  - 0887-2333 (Linking)
VI  - 62
DP  - 2020 Feb
TI  - Immune-competent in vitro co-culture models as an approach for skin sensitisation 
      assessment.
PG  - 104691
LID - S0887-2333(19)30537-5 [pii]
LID - 10.1016/j.tiv.2019.104691 [doi]
AB  - There is a complex interplay between numerous cell types that act at different 
      steps of the mechanism leading to allergic contact dermatitis. The validated in 
      vitro methods for skin sensitisation assessment provide good statistical 
      correspondence to local lymph node assay (LLNA) or to human data but, for the 
      most part, poorly represent the actual in vivo situation as they generally 
      involve single cell type culture in 2D. Significant progress has been made over 
      the past decades to develop new technologies of data generation concurrently with 
      novel approaches to improve the models especially by the use of co-culture. The 
      importance of heterotypic cell-cell interactions in the in vitro assessment of 
      skin sensitisation should not be overlooked. This review addresses the technical 
      aspects to take into consideration when co-culturing depending on the desired 
      objective and describes the different keratinocytes and dendritic cells 
      co-cultures developed in 2D and 3D. To date, from a regulatory point of view, no 
      alternative method to animal testing for skin sensitisation potential assessment 
      using a keratinocytes and dendritic cells co-culture model is yet proposed. This 
      review also presents several directions of further development.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Thelu, Amelie
AU  - Thelu A
AD  - Department of In Vitro Toxicology, Thor Personal Care, Compiegne, France; 
      Inflammation, Chimiokines et Immunopathologie, INSERM UMR996, University 
      Paris-Sud, Universite Paris-Saclay, Chatenay-Malabry, France.
FAU - Catoire, Sophie
AU  - Catoire S
AD  - Department of In Vitro Toxicology, Thor Personal Care, Compiegne, France.
FAU - Kerdine-Romer, Saadia
AU  - Kerdine-Romer S
AD  - Inflammation, Chimiokines et Immunopathologie, INSERM UMR996, University 
      Paris-Sud, Universite Paris-Saclay, Chatenay-Malabry, France. Electronic address: 
      saadia.kerdine-romer@u-psud.fr.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20191022
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
SB  - IM
MH  - Coculture Techniques/*methods
MH  - Dermatitis, Allergic Contact/*immunology
MH  - Humans
MH  - *Immunization
MH  - Immunocompetence/*immunology
MH  - Models, Biological
MH  - Predictive Value of Tests
OTO - NOTNLM
OT  - Co-culture
OT  - Predictive test
OT  - Skin sensitisation
EDAT- 2019/10/28 06:00
MHDA- 2020/06/09 06:00
CRDT- 2019/10/25 06:00
PHST- 2019/07/12 00:00 [received]
PHST- 2019/10/05 00:00 [revised]
PHST- 2019/10/14 00:00 [accepted]
PHST- 2019/10/28 06:00 [pubmed]
PHST- 2020/06/09 06:00 [medline]
PHST- 2019/10/25 06:00 [entrez]
AID - S0887-2333(19)30537-5 [pii]
AID - 10.1016/j.tiv.2019.104691 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2020 Feb;62:104691. doi: 10.1016/j.tiv.2019.104691. Epub 2019 
      Oct 22.