PMID- 31648104
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210110
IS  - 2162-2531 (Print)
IS  - 2162-2531 (Electronic)
IS  - 2162-2531 (Linking)
VI  - 18
DP  - 2019 Dec 6
TI  - Blocking lncRNA MALAT1/miR-199a/ZHX1 Axis Inhibits Glioblastoma Proliferation and 
      Progression.
PG  - 388-399
LID - S2162-2531(19)30250-1 [pii]
LID - 10.1016/j.omtn.2019.09.005 [doi]
AB  - Zinc fingers and homeoboxes 1 (ZHX1) is a transcription repressor that has been 
      implicated in the tumorigenesis and progression of diverse tumors. The functional 
      role and regulating mechanism of ZHX1 has not been elucidated in glioblastoma 
      (GBM). Previous reports have suggested that a large number of non-coding RNAs 
      play a vital role in glioma initiation and progression. This study aimed to 
      investigate the functional role and co-regulatory mechanisms of the 
      metastasis-associated lung adenocarcinoma transcript-1 (MALAT1)/ microRNA-199a 
      (miR-199a)/ZHX1 axis in GBM. We analyzed the expression of the 
      MALAT1/miR-199a/ZHX1 axis and its correlation with patients' overall survival 
      using two different glioma gene-expression datasets. A series of in vitro and 
      in vivo studies including dual luciferase reporter assay, fluorescence in situ 
      hybridization (FISH), RNA immunoprecipitation, and pull-down experiments were 
      completed to elucidate the biological significance of the MALAT1/miR-199a/ZHX1 
      axis in promoting glioma proliferation and progression. Elevated ZHX1 expression 
      correlated with poor prognosis in GBM patients, and in vitro studies demonstrated 
      that ZHX1 attenuated GBM cell apoptosis by downregulation of pro-apoptotic 
      protein (Bax) and upregulation of anti-apoptotic protein (Bcl-2). Furthermore, 
      knockdown of MALAT1 inhibited GBM proliferation and progression in vitro and 
      reduced tumor volume and prolonged survival in an orthotopic GBM murine model. 
      Finally, we demonstrated that MALAT1 promoted ZHX1 expression via acting as a 
      competing endogenous RNA by sponging miR-199a. The MALAT1/miR-199a/ZHX1 axis 
      promotes GBM cell proliferation and progression in vitro and in vivo, and its 
      expression negatively correlates with GBM patient survival. Blocking the 
      MALAT1/miR-199a/ZHX1 axis can serve as a novel therapeutic strategy for treating 
      GBM.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Liao, Keman
AU  - Liao K
AD  - Department of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiaotong 
      University, No. 160, Pujian Road, District Pudong, Shanghai 200127, China.
FAU - Lin, Yingying
AU  - Lin Y
AD  - Department of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiaotong 
      University, No. 160, Pujian Road, District Pudong, Shanghai 200127, China.
FAU - Gao, Weizhen
AU  - Gao W
AD  - Department of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiaotong 
      University, No. 160, Pujian Road, District Pudong, Shanghai 200127, China.
FAU - Xiao, Zhipeng
AU  - Xiao Z
AD  - Department of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiaotong 
      University, No. 160, Pujian Road, District Pudong, Shanghai 200127, China.
FAU - Medina, Rogelio
AU  - Medina R
AD  - Neuro-Oncology Branch, Center for Cancer Research, National Cancer Institute, 
      NIH, Bethesda, MD 20892, USA.
FAU - Dmitriev, Pauline
AU  - Dmitriev P
AD  - Neuro-Oncology Branch, Center for Cancer Research, National Cancer Institute, 
      NIH, Bethesda, MD 20892, USA.
FAU - Cui, Jing
AU  - Cui J
AD  - Neuro-Oncology Branch, Center for Cancer Research, National Cancer Institute, 
      NIH, Bethesda, MD 20892, USA.
FAU - Zhuang, Zhengping
AU  - Zhuang Z
AD  - Neuro-Oncology Branch, Center for Cancer Research, National Cancer Institute, 
      NIH, Bethesda, MD 20892, USA.
FAU - Zhao, Xiaochun
AU  - Zhao X
AD  - Department of Neurosurgery, Barrow Neurological Institute, St. Joseph's Hospital 
      and Medical Center, 350 W. Thomas Road, Phoenix, AZ 85013, USA.
FAU - Qiu, Yongming
AU  - Qiu Y
AD  - Department of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiaotong 
      University, No. 160, Pujian Road, District Pudong, Shanghai 200127, China.
FAU - Zhang, Xiaohua
AU  - Zhang X
AD  - Department of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiaotong 
      University, No. 160, Pujian Road, District Pudong, Shanghai 200127, China. 
      Electronic address: 157223552@qq.com.
FAU - Ge, Jianwei
AU  - Ge J
AD  - Department of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiaotong 
      University, No. 160, Pujian Road, District Pudong, Shanghai 200127, China.
FAU - Guo, Liemei
AU  - Guo L
AD  - Department of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiaotong 
      University, No. 160, Pujian Road, District Pudong, Shanghai 200127, China. 
      Electronic address: guolm001@126.com.
LA  - eng
PT  - Journal Article
DEP - 20190917
PL  - United States
TA  - Mol Ther Nucleic Acids
JT  - Molecular therapy. Nucleic acids
JID - 101581621
PMC - PMC6819876
OTO - NOTNLM
OT  - MALAT1
OT  - ZHX1
OT  - competing endogenous RNA
OT  - glioblastoma
OT  - long non-coding RNA
EDAT- 2019/10/28 06:00
MHDA- 2019/10/28 06:01
PMCR- 2019/09/17
CRDT- 2019/10/25 06:00
PHST- 2019/01/24 00:00 [received]
PHST- 2019/08/18 00:00 [revised]
PHST- 2019/09/10 00:00 [accepted]
PHST- 2019/10/28 06:00 [pubmed]
PHST- 2019/10/28 06:01 [medline]
PHST- 2019/10/25 06:00 [entrez]
PHST- 2019/09/17 00:00 [pmc-release]
AID - S2162-2531(19)30250-1 [pii]
AID - 10.1016/j.omtn.2019.09.005 [doi]
PST - ppublish
SO  - Mol Ther Nucleic Acids. 2019 Dec 6;18:388-399. doi: 10.1016/j.omtn.2019.09.005. 
      Epub 2019 Sep 17.