PMID- 31649651
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 10
DP  - 2019
TI  - MYH9 Aggregation Induced by Direct Interaction With PRRSV GP5 Ectodomain 
      Facilitates Viral Internalization by Permissive Cells.
PG  - 2313
LID - 10.3389/fmicb.2019.02313 [doi]
LID - 2313
AB  - Prevention and control of infection by porcine reproductive and respiratory 
      syndrome virus (PRRSV) remains a challenge, due to our limited understanding of 
      the PRRSV invasion mechanism. Our previous study has shown that PRRSV 
      glycoprotein GP5 interacts with MYH9 C-terminal domain protein (PRA). Here we 
      defined that the first ectodomain of GP5 (GP5-ecto-1) directly interacted with 
      PRA and this interaction triggered PRA and endogenous MYH9 to form filament 
      assembly. More importantly, MYH9 filament assembly was also formed in 
      GP5-ecto-1-transfected MARC-145 cells. Notably, PRRSV infection of MARC-145 cells 
      and porcine alveolar macrophages also induced endogenous MYH9 aggregation and 
      polymerization that were required for subsequent PRRSV internalization. Moreover, 
      overexpression of S100A4, a MYH9-specific disassembly inducer, in MARC-145 cells 
      significantly resulted in diminished MYH9 aggregation and marked inhibition of 
      subsequent virion internalization and infection by both PRRSV-1 and PRRSV-2 
      isolates. The collective results of this work reveal a novel molecular mechanism 
      employed by MYH9 that helps PRRSV gain entry into permissive cells.
CI  - Copyright (c) 2019 Xue, Hou, Zhang, Huang, Li, Nan, Mu, Wang, Zhang, Han, Ren, 
      Zhao, Wu, Wang and Zhou.
FAU - Xue, Biyun
AU  - Xue B
AD  - College of Veterinary Medicine, Northwest A&F University, Xianyang, China.
FAU - Hou, Gaopeng
AU  - Hou G
AD  - College of Veterinary Medicine, Northwest A&F University, Xianyang, China.
FAU - Zhang, Guixi
AU  - Zhang G
AD  - College of Veterinary Medicine, Northwest A&F University, Xianyang, China.
FAU - Huang, Jingjing
AU  - Huang J
AD  - College of Veterinary Medicine, Northwest A&F University, Xianyang, China.
FAU - Li, Liangliang
AU  - Li L
AD  - College of Veterinary Medicine, Northwest A&F University, Xianyang, China.
FAU - Nan, Yuchen
AU  - Nan Y
AD  - College of Veterinary Medicine, Northwest A&F University, Xianyang, China.
FAU - Mu, Yang
AU  - Mu Y
AD  - College of Veterinary Medicine, Northwest A&F University, Xianyang, China.
FAU - Wang, Lizhen
AU  - Wang L
AD  - College of Veterinary Medicine, Northwest A&F University, Xianyang, China.
FAU - Zhang, Lu
AU  - Zhang L
AD  - College of Veterinary Medicine, Northwest A&F University, Xianyang, China.
FAU - Han, Ximeng
AU  - Han X
AD  - College of Veterinary Medicine, Northwest A&F University, Xianyang, China.
FAU - Ren, Xiaolei
AU  - Ren X
AD  - College of Veterinary Medicine, Northwest A&F University, Xianyang, China.
FAU - Zhao, Qin
AU  - Zhao Q
AD  - College of Veterinary Medicine, Northwest A&F University, Xianyang, China.
FAU - Wu, Chunyan
AU  - Wu C
AD  - College of Veterinary Medicine, Northwest A&F University, Xianyang, China.
FAU - Wang, Jingfei
AU  - Wang J
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Zhou, En-Min
AU  - Zhou EM
AD  - College of Veterinary Medicine, Northwest A&F University, Xianyang, China.
LA  - eng
PT  - Journal Article
DEP - 20191009
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC6794372
OTO - NOTNLM
OT  - GP5
OT  - MYH9
OT  - PRRSV
OT  - protein-protein interaction
OT  - virus internalization
EDAT- 2019/10/28 06:00
MHDA- 2019/10/28 06:01
PMCR- 2019/10/09
CRDT- 2019/10/26 06:00
PHST- 2019/08/15 00:00 [received]
PHST- 2019/09/23 00:00 [accepted]
PHST- 2019/10/26 06:00 [entrez]
PHST- 2019/10/28 06:00 [pubmed]
PHST- 2019/10/28 06:01 [medline]
PHST- 2019/10/09 00:00 [pmc-release]
AID - 10.3389/fmicb.2019.02313 [doi]
PST - epublish
SO  - Front Microbiol. 2019 Oct 9;10:2313. doi: 10.3389/fmicb.2019.02313. eCollection 
      2019.