PMID- 31652920
OWN - NLM
STAT- MEDLINE
DCOM- 20200415
LR  - 20240109
IS  - 1660-3397 (Electronic)
IS  - 1660-3397 (Linking)
VI  - 17
IP  - 11
DP  - 2019 Oct 24
TI  - Pyrogallol-Phloroglucinol-6,6-Bieckol from Ecklonia cava Attenuates Tubular 
      Epithelial Cell (TCMK-1) Death in Hypoxia/Reoxygenation Injury.
LID - 10.3390/md17110602 [doi]
LID - 602
AB  - The hypoxia/reoxygenation (H/R) injury causes serious complications after the 
      blood supply to the kidney is stopped during surgery. The main mechanism of I/R 
      injury is the release of high-mobility group protein B1 (HMGB1) from injured 
      tubular epithelial cells (TEC, TCMK-1 cell), which triggers TLR4 or RAGE 
      signaling, leading to cell death. We evaluated whether the extracts of Ecklonia 
      cava (E. cava) would attenuate TEC death induced by H/R injury. We also evaluated 
      which phlorotannin-dieckol (DK), phlorofucofuroeckol A (PFFA), pyrogallol 
      phloroglucinol-6,6-bieckol (PPB), or 2,7-phloroglucinol-6,6-bieckol (PHB)-would 
      have the most potent effect in the context of H/R injury. We used for pre-hypoxia 
      treatment, in which the phlorotannins from E. cava extracts were added before the 
      onset of hypoxia, and a post- hypoxia treatment, in which the phlorotannins were 
      added before the start of reperfusion. PPB most effectively reduced HMGB1 release 
      and the expression of TLR4 and RAGE induced by H/R injury in both pre- and 
      post-hypoxia treatment. PPB also most effectively inhibited the expression of 
      NF-kB and release of the inflammatory cytokines TNF-alpha and IL-6 in both models. 
      PPB most effectively inhibited cell death and expression of cell death signaling 
      molecules such as Erk/pErk, JNK/pJNK, and p38/pp38. These results suggest that 
      PPB blocks the HGMB1-TLR4/RAGE signaling pathway and decreases TEC death induced 
      by H/R and that PPB can be a novel target for renal H/R injury therapy.
FAU - Son, Myeongjoo
AU  - Son M
AUID- ORCID: 0000-0002-9104-3991
AD  - Department of Anatomy & Cell Biology, Gachon University College of Medicine, 
      Incheon 21936, Korea. mjson@gachon.ac.kr.
AD  - Functional Cellular Networks Laboratory, College of Medicine, Department of 
      Medicine, Graduate School and Lee Gil Ya Cancer and Diabetes Institute, Gachon 
      University, Incheon 21999, Korea. mjson@gachon.ac.kr.
FAU - Oh, Seyeon
AU  - Oh S
AD  - Functional Cellular Networks Laboratory, College of Medicine, Department of 
      Medicine, Graduate School and Lee Gil Ya Cancer and Diabetes Institute, Gachon 
      University, Incheon 21999, Korea. seyeon8965@gachon.ac.kr.
FAU - Choi, Chang Hu
AU  - Choi CH
AD  - Department of Thoracic and Cardiovascular Surgery, Gachon University Gil Medical 
      Center, Gachon University, Incheon 21565, Korea. cch624@gilhospital.com.
FAU - Park, Kook Yang
AU  - Park KY
AD  - Department of Thoracic and Cardiovascular Surgery, Gachon University Gil Medical 
      Center, Gachon University, Incheon 21565, Korea. kkyypark@gilhospital.com.
FAU - Son, Kuk Hui
AU  - Son KH
AD  - Department of Thoracic and Cardiovascular Surgery, Gachon University Gil Medical 
      Center, Gachon University, Incheon 21565, Korea. dr632@gilhospital.com.
FAU - Byun, Kyunghee
AU  - Byun K
AD  - Department of Anatomy & Cell Biology, Gachon University College of Medicine, 
      Incheon 21936, Korea. khbyun1@gachon.ac.kr.
AD  - Functional Cellular Networks Laboratory, College of Medicine, Department of 
      Medicine, Graduate School and Lee Gil Ya Cancer and Diabetes Institute, Gachon 
      University, Incheon 21999, Korea. khbyun1@gachon.ac.kr.
LA  - eng
GR  - 20170285/Ministry of Oceans and Fisheries/
PT  - Journal Article
DEP - 20191024
PL  - Switzerland
TA  - Mar Drugs
JT  - Marine drugs
JID - 101213729
RN  - 0 (Cytokines)
RN  - 0 (Dioxins)
RN  - 0 (HMGB1 Protein)
RN  - 0 (NF-kappa B)
RN  - 0 (Tannins)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 4)
RN  - 01Y4A2QXY0 (Pyrogallol)
RN  - 88095-81-2 (6,6'-bieckol)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cytokines/metabolism
MH  - Dioxins/pharmacology
MH  - Epithelial Cells/drug effects
MH  - HMGB1 Protein/metabolism
MH  - Hypoxia/chemically induced/*drug therapy
MH  - Kidney/metabolism
MH  - MAP Kinase Signaling System/drug effects
MH  - Mice
MH  - NF-kappa B/metabolism
MH  - Phaeophyceae/*chemistry
MH  - Pyrogallol/*pharmacology
MH  - Signal Transduction
MH  - Tannins/pharmacology
MH  - Toll-Like Receptor 4/metabolism
PMC - PMC6891818
OTO - NOTNLM
OT  - Ecklonia cava
OT  - ischemia-reperfusion injury
OT  - kidney
OT  - phlorotannins
COIS- The authors have no conflict of interest to declare.
EDAT- 2019/10/28 06:00
MHDA- 2020/04/16 06:00
PMCR- 2019/11/01
CRDT- 2019/10/27 06:00
PHST- 2019/09/25 00:00 [received]
PHST- 2019/10/21 00:00 [revised]
PHST- 2019/10/22 00:00 [accepted]
PHST- 2019/10/27 06:00 [entrez]
PHST- 2019/10/28 06:00 [pubmed]
PHST- 2020/04/16 06:00 [medline]
PHST- 2019/11/01 00:00 [pmc-release]
AID - md17110602 [pii]
AID - marinedrugs-17-00602 [pii]
AID - 10.3390/md17110602 [doi]
PST - epublish
SO  - Mar Drugs. 2019 Oct 24;17(11):602. doi: 10.3390/md17110602.