PMID- 31653482
OWN - NLM
STAT- MEDLINE
DCOM- 20200122
LR  - 20200122
IS  - 1097-4180 (Electronic)
IS  - 1074-7613 (Linking)
VI  - 51
IP  - 5
DP  - 2019 Nov 19
TI  - Genome-wide Analyses of Chromatin State in Human Mast Cells Reveal Molecular 
      Drivers and Mediators of Allergic and Inflammatory Diseases.
PG  - 949-965.e6
LID - S1074-7613(19)30417-0 [pii]
LID - 10.1016/j.immuni.2019.09.021 [doi]
AB  - Mast cells (MCs) are versatile immune cells capable of rapidly responding to a 
      diverse range of extracellular cues. Here, we mapped the genomic and 
      transcriptomic changes in human MCs upon diverse stimuli. Our analyses revealed 
      broad H3K4me3 domains and enhancers associated with activation. Notably, the rise 
      of intracellular calcium concentration upon immunoglobulin E (IgE)-mediated 
      crosslinking of the high-affinity IgE receptor (FcepsilonRI) resulted in genome-wide 
      reorganization of the chromatin landscape and was associated with a specific 
      chromatin signature, which we term Ca(2+)-dependent open chromatin (COC) domains. 
      Examination of differentially expressed genes revealed potential effectors of MC 
      function, and we provide evidence for fibrinogen-like protein 2 (FGL2) as an MC 
      mediator with potential relevance in chronic spontaneous urticaria. 
      Disease-associated single-nucleotide polymorphisms mapped onto cis-regulatory 
      regions of human MCs suggest that MC function may impact a broad range of 
      pathologies. The datasets presented here constitute a resource for the further 
      study of MC function.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Cildir, Gokhan
AU  - Cildir G
AD  - Centre for Cancer Biology, University of South Australia and SA Pathology, 
      Adelaide, SA 5000, Australia; Laboratory of NF-kappaB Signaling, Institute of 
      Molecular and Cell Biology (IMCB), 61 Biopolis Drive, Proteos, Singapore 138673, 
      Singapore.
FAU - Toubia, John
AU  - Toubia J
AD  - Centre for Cancer Biology, University of South Australia and SA Pathology, 
      Adelaide, SA 5000, Australia; ACRF Cancer Genomics Facility, Centre for Cancer 
      Biology, University of South Australia and SA Pathology, Adelaide, SA 5000, 
      Australia.
FAU - Yip, Kwok Ho
AU  - Yip KH
AD  - Centre for Cancer Biology, University of South Australia and SA Pathology, 
      Adelaide, SA 5000, Australia.
FAU - Zhou, Mingyan
AU  - Zhou M
AD  - Centre for Cancer Biology, University of South Australia and SA Pathology, 
      Adelaide, SA 5000, Australia.
FAU - Pant, Harshita
AU  - Pant H
AD  - School of Medicine, University of Adelaide, Adelaide, SA, Australia.
FAU - Hissaria, Pravin
AU  - Hissaria P
AD  - Royal Adelaide Hospital, Adelaide, SA 5000, Australia.
FAU - Zhang, Jingxian
AU  - Zhang J
AD  - Institute of Molecular and Cell Biology (IMCB), 61 Biopolis Drive, Proteos, 
      Singapore 138673, Singapore.
FAU - Hong, Wanjin
AU  - Hong W
AD  - Institute of Molecular and Cell Biology (IMCB), 61 Biopolis Drive, Proteos, 
      Singapore 138673, Singapore.
FAU - Robinson, Nirmal
AU  - Robinson N
AD  - Centre for Cancer Biology, University of South Australia and SA Pathology, 
      Adelaide, SA 5000, Australia.
FAU - Grimbaldeston, Michele A
AU  - Grimbaldeston MA
AD  - OMNI-Biomarker Development, Genentech Inc, South San Francisco, CA 94080, USA.
FAU - Lopez, Angel F
AU  - Lopez AF
AD  - Centre for Cancer Biology, University of South Australia and SA Pathology, 
      Adelaide, SA 5000, Australia.
FAU - Tergaonkar, Vinay
AU  - Tergaonkar V
AD  - Centre for Cancer Biology, University of South Australia and SA Pathology, 
      Adelaide, SA 5000, Australia; Laboratory of NF-kappaB Signaling, Institute of 
      Molecular and Cell Biology (IMCB), 61 Biopolis Drive, Proteos, Singapore 138673, 
      Singapore; Department of Pathology, Yong Loo Lin School of Medicine, National 
      University of Singapore, Singapore 119074, Singapore. Electronic address: 
      vinayt@imcb.a-star.edu.sg.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191022
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Biomarkers)
RN  - 0 (Chromatin)
RN  - 0 (FGL2 protein, human)
RN  - 0 (Histones)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9001-32-5 (Fibrinogen)
SB  - IM
MH  - Biomarkers
MH  - Cells, Cultured
MH  - Chromatin/*genetics/metabolism
MH  - Chromatin Assembly and Disassembly
MH  - *Disease Susceptibility
MH  - Fibrinogen/genetics/metabolism
MH  - Gene Expression Profiling
MH  - *Genome-Wide Association Study
MH  - *Genomics/methods
MH  - Histones/metabolism
MH  - Humans
MH  - Hypersensitivity/etiology/metabolism
MH  - Immunoglobulin E/immunology
MH  - Inflammation/etiology/metabolism
MH  - Mast Cells/*immunology/*metabolism
MH  - Polymorphism, Single Nucleotide
OTO - NOTNLM
OT  - COC domains
OT  - SNPs
OT  - broad H3K4me3 domains
OT  - enhancers
OT  - human mast cells
EDAT- 2019/10/28 06:00
MHDA- 2020/01/23 06:00
CRDT- 2019/10/27 06:00
PHST- 2018/02/28 00:00 [received]
PHST- 2019/02/18 00:00 [revised]
PHST- 2019/09/25 00:00 [accepted]
PHST- 2019/10/28 06:00 [pubmed]
PHST- 2020/01/23 06:00 [medline]
PHST- 2019/10/27 06:00 [entrez]
AID - S1074-7613(19)30417-0 [pii]
AID - 10.1016/j.immuni.2019.09.021 [doi]
PST - ppublish
SO  - Immunity. 2019 Nov 19;51(5):949-965.e6. doi: 10.1016/j.immuni.2019.09.021. Epub 
      2019 Oct 22.