PMID- 31654705
OWN - NLM
STAT- MEDLINE
DCOM- 20200106
LR  - 20200106
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 725
DP  - 2020 Jan 30
TI  - MicroRNA 16-5p is upregulated in calorie-restricted mice and modulates 
      inflammatory cytokines of macrophages.
PG  - 144191
LID - S0378-1119(19)30850-9 [pii]
LID - 10.1016/j.gene.2019.144191 [doi]
AB  - Caloric restriction (CR) has long been known to increase median and maximal 
      lifespans and to decrease mortality and morbidity in short-lived animal models, 
      likely by altering fundamental biological processes that regulate aging and 
      longevity. However, the detailed mechanisms of immunomodulation by CR remain 
      unclear. In this study, we established a mouse model for CR and analyzed the 
      changes of immune cells in these mice. The CR mice fed a calorie-restricted diet 
      for 4 weeks had lower body weight and fat mass compared with control mice. The 
      proportions of CD4(+), CD8(+), and naive CD4(+) T cells in spleen cells from CR 
      mice were higher than those in of control mice. Additionally, the proportion of 
      CD8(+) T cells was significantly decreased and the mRNA expression of 
      proinflammatory cytokines in the colon of CR mice was significantly decreased 
      compared with those of control mice. To determine the effect of CR on microRNA 
      (miRNA) expression, serum and tissues were collected from mice and the expression 
      level of miRNA was analyzed by real-time RT-PCR. As a result, the expressions of 
      miR-16-5p, miR-196b-5p, and miR-218-5p in serum from CR mice were higher than 
      those in control mice. The expression of miR-16-5p increased in the spleen, 
      thymus, colon, and stomach of CR mice compared with expression in control mice. 
      Furthermore, RAW264 cells transfected with a miR-16-5p mimic significantly 
      decreased the mRNA expression of IL-1beta, IL-6, and TNF-alpha under LPS stimulation. 
      These results suggested that miR-16-5p might be a critical factor involving the 
      anti-inflammatory effects of calorie-restricted feeding.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Yamada, Kazuki
AU  - Yamada K
AD  - Department of Bioscience and Biotechnology, Graduate School of Agriculture, 
      Shinshu University, Minamiminowa, Nagano 399-4598, Japan.
FAU - Takizawa, Seiya
AU  - Takizawa S
AD  - Department of Bioscience and Biotechnology, Graduate School of Agriculture, 
      Shinshu University, Minamiminowa, Nagano 399-4598, Japan.
FAU - Ohgaku, Yuki
AU  - Ohgaku Y
AD  - Department of Bioscience and Biotechnology, Graduate School of Agriculture, 
      Shinshu University, Minamiminowa, Nagano 399-4598, Japan.
FAU - Asami, Takuya
AU  - Asami T
AD  - Department of Bioscience and Biotechnology, Graduate School of Agriculture, 
      Shinshu University, Minamiminowa, Nagano 399-4598, Japan.
FAU - Furuya, Kanon
AU  - Furuya K
AD  - Department of Bioscience and Biotechnology, Graduate School of Agriculture, 
      Shinshu University, Minamiminowa, Nagano 399-4598, Japan.
FAU - Yamamoto, Kana
AU  - Yamamoto K
AD  - Department of Bioscience and Biotechnology, Graduate School of Agriculture, 
      Shinshu University, Minamiminowa, Nagano 399-4598, Japan.
FAU - Takahashi, Fuka
AU  - Takahashi F
AD  - Department of Bioscience and Biotechnology, Graduate School of Agriculture, 
      Shinshu University, Minamiminowa, Nagano 399-4598, Japan.
FAU - Hamajima, Chisato
AU  - Hamajima C
AD  - Department of Bioscience and Biotechnology, Graduate School of Agriculture, 
      Shinshu University, Minamiminowa, Nagano 399-4598, Japan.
FAU - Inaba, Chihiro
AU  - Inaba C
AD  - Department of Bioscience and Biotechnology, Graduate School of Agriculture, 
      Shinshu University, Minamiminowa, Nagano 399-4598, Japan.
FAU - Endo, Katsunori
AU  - Endo K
AD  - Department of Bioscience and Biotechnology, Graduate School of Agriculture, 
      Shinshu University, Minamiminowa, Nagano 399-4598, Japan.
FAU - Matsui, Rina
AU  - Matsui R
AD  - Department of Bioscience and Biotechnology, Graduate School of Agriculture, 
      Shinshu University, Minamiminowa, Nagano 399-4598, Japan.
FAU - Kitamura, Hidemitsu
AU  - Kitamura H
AD  - Division of Functional Immunology, Section of Disease Control, Institute for 
      Genetic Medicine, Hokkaido University, Sapporo 090-0815, Japan.
FAU - Tanaka, Sachi
AU  - Tanaka S
AD  - Department of Bioscience and Biotechnology, Graduate School of Agriculture, 
      Shinshu University, Minamiminowa, Nagano 399-4598, Japan. Electronic address: 
      tanakasa@shinshu-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20191022
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1beta)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn16 microRNA, mouse)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Aging/metabolism
MH  - Animals
MH  - Caloric Restriction/methods
MH  - Cytokines/genetics/metabolism
MH  - Diet Therapy
MH  - Inflammation/metabolism
MH  - Interleukin-1beta/genetics
MH  - Macrophages/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*genetics/*metabolism
MH  - Models, Animal
MH  - RAW 264.7 Cells
MH  - Transcriptional Activation
MH  - Tumor Necrosis Factor-alpha/genetics
MH  - Up-Regulation
OTO - NOTNLM
OT  - Caloric restriction
OT  - Cellular immunology
OT  - Cytokines
OT  - Macrophages
OT  - microRNA
EDAT- 2019/10/28 06:00
MHDA- 2020/01/07 06:00
CRDT- 2019/10/27 06:00
PHST- 2019/06/25 00:00 [received]
PHST- 2019/10/18 00:00 [revised]
PHST- 2019/10/18 00:00 [accepted]
PHST- 2019/10/28 06:00 [pubmed]
PHST- 2020/01/07 06:00 [medline]
PHST- 2019/10/27 06:00 [entrez]
AID - S0378-1119(19)30850-9 [pii]
AID - 10.1016/j.gene.2019.144191 [doi]
PST - ppublish
SO  - Gene. 2020 Jan 30;725:144191. doi: 10.1016/j.gene.2019.144191. Epub 2019 Oct 22.