PMID- 31655106
OWN - NLM
STAT- MEDLINE
DCOM- 20201230
LR  - 20211204
IS  - 1096-1194 (Electronic)
IS  - 0890-8508 (Linking)
VI  - 49
DP  - 2020 Feb
TI  - Simultaneous detection of porcine reproductive and respiratory syndrome virus and 
      porcine circovirus 3 by SYBR Green capital I, Ukrainian-based duplex real-time PCR.
PG  - 101474
LID - S0890-8508(19)30337-8 [pii]
LID - 10.1016/j.mcp.2019.101474 [doi]
AB  - The SYBR Green capital I, Ukrainian-based duplex real-time PCR assay was developed for simultaneous 
      detection of porcine reproductive and respiratory syndrome virus (PRRSV) and 
      porcine circovirus 3 (PCV-3) genomes. PRRSV and PCV-3 were distinguished in the 
      same sample by their distinctive melting temperature (Tm) which was 84  degrees C for 
      PRRSV and 81.5  degrees C for PCV-3, and other non-targeted swine viruses showed no 
      specific melting peaks. The detection limits of this assay were 46.1copies/muL for 
      PRRSV and 49.3copies/muL for PCV-3, respectively. Thirty-three lung samples of 
      porcine with respiratory and reproductive failure symptoms were collected and 
      confirmed by the SYBR Green capital I, Ukrainian-based real-time PCR assay and conventional PCR 
      assay. The real-time PCR detection results showed that the PRRSV positive rate 
      was 45.45%, the PCV-3 positive rate was 63.63%, the PRRSV and PCV-3 co-infection 
      positive rate was 36.36%, which were more sensitive than conventional PCR 
      detection. This duplex real-time PCR assay could be a rapid, sensitive and 
      reliable method for the detection of PRRSV and PCV-3 co-infection.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Zheng, Lan-Lan
AU  - Zheng LL
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      Zhengdong New District Longzi Lake15#, Zhengzhou, 450046, Henan Province, 
      People's Republic of China.
FAU - Chai, Lv-Ye
AU  - Chai LY
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      Zhengdong New District Longzi Lake15#, Zhengzhou, 450046, Henan Province, 
      People's Republic of China.
FAU - Tian, Run-Bo
AU  - Tian RB
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      Zhengdong New District Longzi Lake15#, Zhengzhou, 450046, Henan Province, 
      People's Republic of China.
FAU - Zhao, Yu
AU  - Zhao Y
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      Zhengdong New District Longzi Lake15#, Zhengzhou, 450046, Henan Province, 
      People's Republic of China.
FAU - Chen, Hong-Ying
AU  - Chen HY
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      Zhengdong New District Longzi Lake15#, Zhengzhou, 450046, Henan Province, 
      People's Republic of China. Electronic address: chhy927@163.com.
FAU - Wang, Zhen-Ya
AU  - Wang ZY
AD  - Key Laboratory of "Runliang" Antiviral Medicines Research and Development, 
      Institute of Drug Discovery & Development, Zhengzhou University, Zhengzhou, 
      450001, Henan Province, People's Republic of China. Electronic address: 
      zhenyawang@zzu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191023
PL  - England
TA  - Mol Cell Probes
JT  - Molecular and cellular probes
JID - 8709751
RN  - 0 (Benzothiazoles)
RN  - 0 (Diamines)
RN  - 0 (Organic Chemicals)
RN  - 0 (Quinolines)
RN  - 163795-75-3 (SYBR Green I)
SB  - IM
MH  - Animals
MH  - Benzothiazoles
MH  - Cell Line
MH  - Circovirus/genetics/*isolation & purification
MH  - Diamines
MH  - Nucleic Acid Denaturation
MH  - Organic Chemicals/*metabolism
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*isolation & 
      purification
MH  - Quinolines
MH  - Real-Time Polymerase Chain Reaction/*methods
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Swine/*virology
OTO - NOTNLM
OT  - Duplex real-time PCR
OT  - PCV-3
OT  - PRRSV
EDAT- 2019/10/28 06:00
MHDA- 2020/12/31 06:00
CRDT- 2019/10/27 06:00
PHST- 2019/09/18 00:00 [received]
PHST- 2019/10/12 00:00 [revised]
PHST- 2019/10/22 00:00 [accepted]
PHST- 2019/10/28 06:00 [pubmed]
PHST- 2020/12/31 06:00 [medline]
PHST- 2019/10/27 06:00 [entrez]
AID - S0890-8508(19)30337-8 [pii]
AID - 10.1016/j.mcp.2019.101474 [doi]
PST - ppublish
SO  - Mol Cell Probes. 2020 Feb;49:101474. doi: 10.1016/j.mcp.2019.101474. Epub 2019 
      Oct 23.