PMID- 31655425
OWN - NLM
STAT- MEDLINE
DCOM- 20200617
LR  - 20200617
IS  - 1872-8421 (Electronic)
IS  - 0165-5728 (Linking)
VI  - 337
DP  - 2019 Dec 15
TI  - A novel MuSK cell-based myasthenia gravis diagnostic assay.
PG  - 577076
LID - S0165-5728(19)30256-5 [pii]
LID - 10.1016/j.jneuroim.2019.577076 [doi]
AB  - To improve the clinical diagnosis of neural autoimmune diseases, we developed an 
      in-house muscle-specific kinase (MuSK) antibody cell-based assay (CBA) and 
      compared its performance with RIA, ELISA, and other CBAs. Sera from patients with 
      myasthenia gravis (MG) and other autoimmune diseases were analyzed. We found 46 
      (18.3%) MuSK-CBA Ab positive cases among 251 AChR-Ab negative cases [patients] 
      and 4 (0.6%) MuSK-CBA Ab positive cases [among] the 624 AChR-Ab positive samples. 
      Comparing these with available clinic assays, our highly specific CBA method is 
      more sensitive than commercial ELISA and IFA(indirect immunofluorescence assay).
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Han, Jiaojiao
AU  - Han J
AD  - Department of Neuroimmunology, Henan Institute of Medical and Pharmaceutical 
      Sciences, Zhengzhou University, Zhengzhou 450052, China; Academy of Medical 
      Sciences, Zhengzhou University, Zhengzhou 450052, China.
FAU - Zhang, Jing
AU  - Zhang J
AD  - Department of Neuroimmunology, Henan Institute of Medical and Pharmaceutical 
      Sciences, Zhengzhou University, Zhengzhou 450052, China.
FAU - Li, Mingqiang
AU  - Li M
AD  - Department of Neurology, The Second Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450014, China.
FAU - Zhang, Yingna
AU  - Zhang Y
AD  - Department of Neuroimmunology, Henan Institute of Medical and Pharmaceutical 
      Sciences, Zhengzhou University, Zhengzhou 450052, China.
FAU - Lv, Jie
AU  - Lv J
AD  - Department of Neuroimmunology, Henan Institute of Medical and Pharmaceutical 
      Sciences, Zhengzhou University, Zhengzhou 450052, China.
FAU - Zhao, Xue
AU  - Zhao X
AD  - Department of Neuroimmunology, Henan Institute of Medical and Pharmaceutical 
      Sciences, Zhengzhou University, Zhengzhou 450052, China.
FAU - Wang, Shumin
AU  - Wang S
AD  - Department of Neuroimmunology, Henan Institute of Medical and Pharmaceutical 
      Sciences, Zhengzhou University, Zhengzhou 450052, China.
FAU - Wang, Lulu
AU  - Wang L
AD  - Department of Neurology, The Second Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450014, China.
FAU - Yang, Haonan
AU  - Yang H
AD  - Department of Neuroimmunology, Henan Institute of Medical and Pharmaceutical 
      Sciences, Zhengzhou University, Zhengzhou 450052, China; BGI College, Zhengzhou 
      University, Zhengzhou 450052, China.
FAU - Han, Sensen
AU  - Han S
AD  - Department of Neuroimmunology, Henan Institute of Medical and Pharmaceutical 
      Sciences, Zhengzhou University, Zhengzhou 450052, China; BGI College, Zhengzhou 
      University, Zhengzhou 450052, China.
FAU - Fang, Hua
AU  - Fang H
AD  - Department of Neuroimmunology, Henan Institute of Medical and Pharmaceutical 
      Sciences, Zhengzhou University, Zhengzhou 450052, China.
FAU - Gao, Feng
AU  - Gao F
AD  - Department of Neuroimmunology, Henan Institute of Medical and Pharmaceutical 
      Sciences, Zhengzhou University, Zhengzhou 450052, China. Electronic address: 
      gaoyuanshan@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191019
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Autoantibodies)
RN  - 0 (Biomarkers)
RN  - 0 (Receptors, Cholinergic)
RN  - EC 2.7.10.1 (MUSK protein, human)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Autoantibodies/*blood
MH  - Biomarkers/blood
MH  - Enzyme-Linked Immunosorbent Assay/methods/standards
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Myasthenia Gravis/*blood/*diagnosis
MH  - Receptor Protein-Tyrosine Kinases/*blood
MH  - Receptors, Cholinergic/*blood
OTO - NOTNLM
OT  - Antibody
OT  - Cell-based assay
OT  - Enzyme-linked immunosorbent assay
OT  - Indirect immunofluorescence assay
OT  - Muscle-specific kinase
OT  - Myasthenia gravis
EDAT- 2019/10/28 06:00
MHDA- 2020/06/18 06:00
CRDT- 2019/10/27 06:00
PHST- 2019/05/22 00:00 [received]
PHST- 2019/09/19 00:00 [revised]
PHST- 2019/09/27 00:00 [accepted]
PHST- 2019/10/28 06:00 [pubmed]
PHST- 2020/06/18 06:00 [medline]
PHST- 2019/10/27 06:00 [entrez]
AID - S0165-5728(19)30256-5 [pii]
AID - 10.1016/j.jneuroim.2019.577076 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2019 Dec 15;337:577076. doi: 10.1016/j.jneuroim.2019.577076. Epub 
      2019 Oct 19.