PMID- 31656212
OWN - NLM
STAT- MEDLINE
DCOM- 20200618
LR  - 20200618
IS  - 1435-8115 (Electronic)
IS  - 1431-9276 (Linking)
VI  - 25
IP  - 6
DP  - 2019 Dec
TI  - In Vivo Multivesicular Body and Exosome Secretion in the Intestinal Epithelial 
      Cells of Turtles During Hibernation.
PG  - 1341-1351
LID - 10.1017/S1431927619015071 [doi]
AB  - The present study was designed to investigate the in vivo biological processes of 
      multivesicular bodies (MVBs) and exosomes in mitochondria-rich cells (MRCs), 
      goblet cells (GCs), and absorptive cells (ACs) in turtle intestines during 
      hibernation. The exosome markers, cluster of differentiation 63 (CD63) and tumor 
      susceptibility gene 101 (TSG101), were positively expressed in intestinal villi 
      during turtle hibernation. The distribution and formation processes of MVBs and 
      exosomes in turtle MRCs, GCs, and ACs were further confirmed by transmission 
      electron microscopy. During hibernation, abundantly secreted early endosomes 
      (ees) were localized in the luminal and basal cytoplasm of the MRCs and ACs, and 
      late endosomes (les) were dispersed with the supranuclear parts of the MRCs and 
      ACs. Many "heterogeneous" MVBs were identified throughout the cytoplasm of the 
      MRCs and ACs. Interestingly, the ees, les, and MVBs were detected in the 
      cytoplasm of the GCs during hibernation; however, they were absent during 
      nonhibernation. Furthermore, the exocytosis pathways of exosomes and autophagic 
      vacuoles were observed in the MRCs, GCs, and ACs during hibernation. In addition, 
      the number of different MVBs with intraluminal vesicles (ILVs) and heterogeneous 
      endosome-MVB-exosome complexes was significantly increased in the MRCs, GCs, and 
      ACs during hibernation. All these findings indicate that intestinal epithelial 
      cells potentially perform a role in the secretion of MVBs and exosomes, which are 
      essential for mucosal immunity, during hibernation.
FAU - Vistro, Waseem Ali
AU  - Vistro WA
AD  - MOE Joint International Research Laboratory of Animal Health and Food safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province 210095, China.
FAU - Huang, Yufei
AU  - Huang Y
AD  - MOE Joint International Research Laboratory of Animal Health and Food safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province 210095, China.
FAU - Bai, Xuebing
AU  - Bai X
AD  - MOE Joint International Research Laboratory of Animal Health and Food safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province 210095, China.
FAU - Yang, Ping
AU  - Yang P
AD  - MOE Joint International Research Laboratory of Animal Health and Food safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province 210095, China.
FAU - Haseeb, Abdul
AU  - Haseeb A
AD  - MOE Joint International Research Laboratory of Animal Health and Food safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province 210095, China.
FAU - Chen, Hong
AU  - Chen H
AD  - MOE Joint International Research Laboratory of Animal Health and Food safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province 210095, China.
FAU - Liu, Yifei
AU  - Liu Y
AD  - MOE Joint International Research Laboratory of Animal Health and Food safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province 210095, China.
FAU - Yue, Zhang
AU  - Yue Z
AD  - MOE Joint International Research Laboratory of Animal Health and Food safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province 210095, China.
FAU - Tarique, Imran
AU  - Tarique I
AD  - MOE Joint International Research Laboratory of Animal Health and Food safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province 210095, China.
FAU - Chen, Qiusheng
AU  - Chen Q
AUID- ORCID: 0000-0002-6087-7029
AD  - MOE Joint International Research Laboratory of Animal Health and Food safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province 210095, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Microsc Microanal
JT  - Microscopy and microanalysis : the official journal of Microscopy Society of 
      America, Microbeam Analysis Society, Microscopical Society of Canada
JID - 9712707
RN  - 0 (Biomarkers)
SB  - IM
MH  - Animals
MH  - Biomarkers/analysis
MH  - Epithelial Cells/*physiology/ultrastructure
MH  - Exosomes/chemistry/*metabolism/ultrastructure
MH  - *Hibernation
MH  - Immunohistochemistry
MH  - Intestinal Mucosa/*physiology
MH  - Microscopy, Electron, Transmission
MH  - Multivesicular Bodies/chemistry/*metabolism/ultrastructure
MH  - *Turtles
OTO - NOTNLM
OT  - Chinese soft-shelled turtle
OT  - absorptive cells
OT  - exosomes
OT  - mucosal-immune response
OT  - multivesicular bodies
EDAT- 2019/10/28 06:00
MHDA- 2020/06/19 06:00
CRDT- 2019/10/29 06:00
PHST- 2019/10/28 06:00 [pubmed]
PHST- 2020/06/19 06:00 [medline]
PHST- 2019/10/29 06:00 [entrez]
AID - S1431927619015071 [pii]
AID - 10.1017/S1431927619015071 [doi]
PST - ppublish
SO  - Microsc Microanal. 2019 Dec;25(6):1341-1351. doi: 10.1017/S1431927619015071.