PMID- 31658957
OWN - NLM
STAT- MEDLINE
DCOM- 20200122
LR  - 20200617
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 88
IP  - 1
DP  - 2019 Dec 17
TI  - Chlamydia psittaci-Infected Dendritic Cells Communicate with NK Cells via 
      Exosomes To Activate Antibacterial Immunity.
LID - 10.1128/IAI.00541-19 [doi]
LID - e00541-19
AB  - Dendritic cells (DCs) and natural killer (NK) cells are critically involved in 
      the early response against various bacterial microbes. Functional activation of 
      infected DCs and NK cell-mediated gamma interferon (IFN-gamma) secretion essentially 
      contribute to the protective immunity against Chlamydia How DCs and NK cells 
      cooperate during the antichlamydial response is not fully understood. Therefore, 
      in the present study, we investigated the functional interplay between 
      Chlamydia-infected DCs and NK cells. Our biochemical and cell biological 
      experiments show that Chlamydia psittaci-infected DCs display enhanced exosome 
      release. We find that such extracellular vesicles (referred to as dexosomes) do 
      not contain infectious bacterial material but strongly induce IFN-gamma production by 
      NK cells. This directly affects C. psittaci growth in infected target cells. 
      Furthermore, NK cell-released IFN-gamma in cooperation with tumor necrosis factor 
      alpha (TNF-alpha) and/or dexosomes augments apoptosis of both noninfected and 
      infected epithelial cells. Thus, the combined effect of dexosomes and 
      proinflammatory cytokines restricts C. psittaci growth and attenuates bacterial 
      subversion of apoptotic host cell death. In conclusion, this provides new 
      insights into the functional cooperation between DCs, dexosomes, and NK cells in 
      the early steps of antichlamydial defense.
CI  - Copyright (c) 2019 American Society for Microbiology.
FAU - Radomski, Nadine
AU  - Radomski N
AD  - Institute of Immunology, Friedrich Loeffler Institute, Federal Research Institute 
      of Animal Health, Greifswald-Isle of Riems, Germany.
FAU - Karger, Axel
AU  - Karger A
AUID- ORCID: 0000-0002-0054-7394
AD  - Institute of Molecular Virology and Cell Biology, Friedrich Loeffler Institute, 
      Federal Research Institute of Animal Health, Greifswald-Isle of Riems, Germany.
FAU - Franzke, Kati
AU  - Franzke K
AD  - Institute of Infectology, Friedrich Loeffler Institute, Federal Research 
      Institute of Animal Health, Greifswald-Isle of Riems, Germany.
FAU - Liebler-Tenorio, Elisabeth
AU  - Liebler-Tenorio E
AD  - Institute of Molecular Pathogenesis, Friedrich Loeffler Institute, Federal 
      Research Institute of Animal Health, Jena, Germany.
FAU - Jahnke, Rico
AU  - Jahnke R
AD  - Institute of Immunology, Friedrich Loeffler Institute, Federal Research Institute 
      of Animal Health, Greifswald-Isle of Riems, Germany.
FAU - Matthiesen, Svea
AU  - Matthiesen S
AD  - Institute of Immunology, Friedrich Loeffler Institute, Federal Research Institute 
      of Animal Health, Greifswald-Isle of Riems, Germany.
FAU - Knittler, Michael R
AU  - Knittler MR
AD  - Institute of Immunology, Friedrich Loeffler Institute, Federal Research Institute 
      of Animal Health, Greifswald-Isle of Riems, Germany michael.knittler@fli.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191217
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Immunologic Factors)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - *Cell Communication
MH  - Cells, Cultured
MH  - Chlamydia Infections/*immunology
MH  - Chlamydophila psittaci/*immunology
MH  - Dendritic Cells/*metabolism
MH  - Exosomes/*metabolism
MH  - *Immunity, Innate
MH  - Immunologic Factors/metabolism
MH  - Interferon-gamma/metabolism
MH  - Killer Cells, Natural/*metabolism
MH  - Mice
MH  - Models, Theoretical
PMC - PMC6921653
OTO - NOTNLM
OT  - Chlamydia
OT  - NK cells
OT  - dendritic cells
OT  - exosomes
EDAT- 2019/10/30 06:00
MHDA- 2020/01/23 06:00
PMCR- 2020/06/17
CRDT- 2019/10/30 06:00
PHST- 2019/07/14 00:00 [received]
PHST- 2019/10/21 00:00 [accepted]
PHST- 2019/10/30 06:00 [pubmed]
PHST- 2020/01/23 06:00 [medline]
PHST- 2019/10/30 06:00 [entrez]
PHST- 2020/06/17 00:00 [pmc-release]
AID - IAI.00541-19 [pii]
AID - 00541-19 [pii]
AID - 10.1128/IAI.00541-19 [doi]
PST - epublish
SO  - Infect Immun. 2019 Dec 17;88(1):e00541-19. doi: 10.1128/IAI.00541-19. Print 2019 
      Dec 17.