PMID- 31660449
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2465-9525 (Print)
IS  - 2465-9541 (Electronic)
IS  - 2465-9525 (Linking)
VI  - 23
IP  - 3
DP  - 2019 Sep
TI  - Dietary Exposure to Transgenic Rice Expressing the Spider Silk Protein Fibroin 
      Reduces Blood Glucose Levels in Diabetic Mice: The Potential Role of Insulin 
      Receptor Substrate-1 Phosphorylation in Adipocytes.
PG  - 223-229
LID - 10.12717/DR.2019.23.3.223 [doi]
AB  - Type 2 diabetes mellitus (T2DM) is characterized by insulin resistance (IR). T2DM 
      is correlated with obesity and most T2DM medications have been developed for 
      enhancing insulin sensitivity. Silk protein fibroin (SPF) from spiders has been 
      suggested as an attractive biomaterial for medical purposes. We generated 
      transgenic rice (TR) expressing SPF and fed it to diabetic BKS.Cg-m+/+Lepr(db) 
      mice to monitor the changes in blood glucose levels and adipose tissue proteins 
      associated with energy metabolism and insulin signaling. In the present study, 
      the adipocyte size in abdominal fat in TR-SPF-fed mice was remarkably smaller 
      than that of the control. Whereas the adenosine monophosphate-activated protein 
      kinase (AMPK)-activated protein kinase and insulin receptor substrate 1 (IRS1) 
      protein levels were increased in abdominal adipose tissues after TR-SPF feeding, 
      levels of six-transmembrane protein of prostate 2 (STAMP2) proteins decreased. 
      Phosphorylation of AMPK at threonine 172 and IRS1 at serine 307 and tyrosine 632 
      were both increased in adipose tissues from TR-SPF-fed mice. Increased expression 
      and phosphorylation of IRS1 at both serine 307 and tyrosine 632 in adipose 
      tissues indicated that adipocytes obtained from abdominal fat in TR-SPF-fed mice 
      were more susceptible to insulin signaling than that of the control. STAMP2 
      protein levels decreased in adipose tissues from TR-SPF-fed mice, indicating that 
      STAMP2 proteins were reducing adipocytes that were undergoing lipolysis. Taken 
      together, this study showed that TR-SPF was effective in reducing blood glucose 
      levels in diabetic mice and that concurrent lipolysis in abdominal adipocytes was 
      associated with alterations of AMPK, IRS1, and STAMP2. Increased IRS1 expression 
      and its phosphorylation by TR-SFP were considered to be particularly important in 
      the induction of lipolysis in adipocytes, as well as in reducing blood glucose 
      levels in this animal model.
CI  - (c) Copyright 2019 The Korean Society of Developmental Biology.
FAU - Park, Ji-Eun
AU  - Park JE
AD  - Dept. of Anatomy and Cell Biology, College of Medicine, Dong-A University, Busan 
      49201, Korea.
FAU - Jeong, Yeon Jae
AU  - Jeong YJ
AD  - Dept. of Anatomy and Cell Biology, College of Medicine, Dong-A University, Busan 
      49201, Korea.
FAU - Park, Joon Beom
AU  - Park JB
AD  - Dept. of Anatomy and Cell Biology, College of Medicine, Dong-A University, Busan 
      49201, Korea.
FAU - Kim, Hye Young
AU  - Kim HY
AD  - Dept. of Anatomy and Cell Biology, College of Medicine, Dong-A University, Busan 
      49201, Korea.
FAU - Yoo, Young Hyun
AU  - Yoo YH
AD  - Dept. of Anatomy and Cell Biology, College of Medicine, Dong-A University, Busan 
      49201, Korea.
FAU - Lee, Kwang Sik
AU  - Lee KS
AD  - College of Life Sciences and Natural Resources, Dong-A University, Busan 49315, 
      Korea.
FAU - Yang, Won Tae
AU  - Yang WT
AD  - College of Life Sciences and Natural Resources, Dong-A University, Busan 49315, 
      Korea.
FAU - Kim, Doh Hoon
AU  - Kim DH
AD  - College of Life Sciences and Natural Resources, Dong-A University, Busan 49315, 
      Korea.
FAU - Kim, Jong-Min
AU  - Kim JM
AD  - Dept. of Anatomy and Cell Biology, College of Medicine, Dong-A University, Busan 
      49201, Korea.
LA  - eng
PT  - Journal Article
DEP - 20190930
PL  - Korea (South)
TA  - Dev Reprod
JT  - Development & reproduction
JID - 101178352
PMC - PMC6812974
OTO - NOTNLM
OT  - Adipocytes
OT  - Diabetic mouse
OT  - IRS1
OT  - Spider silk protein fibroin
OT  - Transgenic rice
COIS- The authors declare no potential conflict of interest.
EDAT- 2019/10/30 06:00
MHDA- 2019/10/30 06:01
PMCR- 2019/09/01
CRDT- 2019/10/30 06:00
PHST- 2019/07/10 00:00 [received]
PHST- 2019/07/31 00:00 [revised]
PHST- 2019/08/13 00:00 [accepted]
PHST- 2019/10/30 06:00 [entrez]
PHST- 2019/10/30 06:00 [pubmed]
PHST- 2019/10/30 06:01 [medline]
PHST- 2019/09/01 00:00 [pmc-release]
AID - dr-23-3-223 [pii]
AID - 10.12717/DR.2019.23.3.223 [doi]
PST - ppublish
SO  - Dev Reprod. 2019 Sep;23(3):223-229. doi: 10.12717/DR.2019.23.3.223. Epub 2019 Sep 
      30.