PMID- 31660453
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2465-9525 (Print)
IS  - 2465-9541 (Electronic)
IS  - 2465-9525 (Linking)
VI  - 23
IP  - 3
DP  - 2019 Sep
TI  - Chronic and Low Dose Exposure to Nonlyphenol or Di(2-Ethylhexyl) Phthalate Alters 
      Cell Proliferation and the Localization of Steroid Hormone Receptors in Uterine 
      Endometria in Mice.
PG  - 263-275
LID - 10.12717/DR.2019.23.3.263 [doi]
AB  - Based on our preliminary results, we examined the possible role of low-dose and 
      chronic-exposing of the chemicals those are known as endocrine disrupting 
      chemical (EDC), on the proliferation of uterine endometrium and the localization 
      of steroid receptors. Immunohistochemical or immunofluorochemical methodology 
      were employed to evaluate the localization of antigen identified by monoclonal 
      antibody Ki 67 protein (MKI67), estrogen receptor 1 (ESR1), estrogen receptor 2 
      (ESR2), and progesterone receptor (PGR). In 133 mug/L and 1,330 mug/L 
      di(2-ethylhexyl) phthalate (DEHP) and 50 mug/L nonylphenol (NP) groups, the ratio 
      of MKI67 positive stromal cells was significantly increased but not in 500 mug/L 
      NP group. The ratios of MKI67 positive glandular and luminal epithelial cells 
      were also changed by the chronic administration of NP and DEHP in tissue with 
      dose specific manner. ESR1 signals were localized in nucleus in glandular and 
      luminal epithelia of control group but its localization was mainly in cytoplasm 
      in DEHP and NP administered groups. On the other hand, it was decreased at 
      nucleus of stromal cells in 1,330 mug/L DEHP group. The colocalization patterns of 
      these nuclear receptors were also modified by the administration of these 
      chemicals. Such a tissue specific and dose specific localization of ESR2 and PGR 
      were detected as ESR1 in all the uterine endometrial tissues. These results show 
      that the chronic lows-dose exposing of NP or DEHP modify the localization and 
      colocalization of ESRs and PGR, and of the proliferation patterns of the 
      endometrial tissues.
CI  - (c) Copyright 2019 The Korean Society of Developmental Biology.
FAU - Kim, Juhye
AU  - Kim J
AD  - Division of Developmental Biology and Physiology, Dept. of Biotechnology, 
      Sungshin University, Seoul 02844, Korea.
FAU - Cha, Sunyeong
AU  - Cha S
AD  - Division of Developmental Biology and Physiology, Dept. of Biotechnology, 
      Sungshin University, Seoul 02844, Korea.
FAU - Lee, Min Young
AU  - Lee MY
AD  - Division of Developmental Biology and Physiology, Dept. of Biotechnology, 
      Sungshin University, Seoul 02844, Korea.
FAU - Hwang, Yeon Jeong
AU  - Hwang YJ
AD  - Division of Developmental Biology and Physiology, Dept. of Biotechnology, 
      Sungshin University, Seoul 02844, Korea.
FAU - Yang, Eunhyeok
AU  - Yang E
AD  - Division of Developmental Biology and Physiology, Dept. of Biotechnology, 
      Sungshin University, Seoul 02844, Korea.
FAU - Choi, Donchan
AU  - Choi D
AD  - Dept. of Life Science, College of Environmental Sciences, Yong-In University, 
      Yongin 17092, Korea.
FAU - Lee, Sung-Ho
AU  - Lee SH
AD  - Dept. of Biotechnology, Sangmyung University, Seoul 03016, Korea.
FAU - Cheon, Yong-Pil
AU  - Cheon YP
AD  - Division of Developmental Biology and Physiology, Dept. of Biotechnology, 
      Sungshin University, Seoul 02844, Korea.
LA  - eng
PT  - Journal Article
DEP - 20190930
PL  - Korea (South)
TA  - Dev Reprod
JT  - Development & reproduction
JID - 101178352
PMC - PMC6812976
OTO - NOTNLM
OT  - Di(2-ethylhexyl) phthalate
OT  - Epithelial cell
OT  - Nonylphenol
OT  - Steroid hormone receptors
OT  - Stromal cell
COIS- The authors declare no potential conflict of interest.
EDAT- 2019/10/30 06:00
MHDA- 2019/10/30 06:01
PMCR- 2019/09/01
CRDT- 2019/10/30 06:00
PHST- 2019/06/08 00:00 [received]
PHST- 2019/08/30 00:00 [revised]
PHST- 2019/09/19 00:00 [accepted]
PHST- 2019/10/30 06:00 [entrez]
PHST- 2019/10/30 06:00 [pubmed]
PHST- 2019/10/30 06:01 [medline]
PHST- 2019/09/01 00:00 [pmc-release]
AID - dr-23-3-263 [pii]
AID - 10.12717/DR.2019.23.3.263 [doi]
PST - ppublish
SO  - Dev Reprod. 2019 Sep;23(3):263-275. doi: 10.12717/DR.2019.23.3.263. Epub 2019 Sep 
      30.