PMID- 31666088
OWN - NLM
STAT- MEDLINE
DCOM- 20200330
LR  - 20201114
IS  - 1742-2094 (Electronic)
IS  - 1742-2094 (Linking)
VI  - 16
IP  - 1
DP  - 2019 Oct 30
TI  - CD147 as a key mediator of the spleen inflammatory response in mice after focal 
      cerebral ischemia.
PG  - 198
LID - 10.1186/s12974-019-1609-y [doi]
LID - 198
AB  - BACKGROUND: The splenic inflammatory response after cerebral ischemia has been 
      implicated in secondary brain injury. We have recently reported that CD147 plays 
      an important role in driving brain inflammation after ischemic stroke. In this 
      study, we hypothesized that CD147 may play a role in the splenic inflammatory 
      response after cerebral ischemia. METHODS: Transient (60 min) middle cerebral 
      artery occlusion was induced in wild-type mice treated with an anti-CD147 
      antibody (alphaCD147) 1 h before ischemia onset. The splenic inflammatory response 
      was evaluated at 4 and 24 h, representing the peak and early stage of splenic 
      inflammatory activation in this model. Changes in mRNA and protein expression of 
      CD147 and inflammatory markers were measured using RT-qPCR and western blot, 
      respectively. Immune cells in the spleen and brain were measured using flow 
      cytometry. RESULTS: CD147 expression was rapidly upregulated in the spleen at 4 
      and 24 h after ischemia onset. The splenic inflammatory response induced by 
      cerebral ischemia was inhibited by alphaCD147 treatment as demonstrated by the 
      reduced expression of cytokines (TNFalpha, IL-6, IL-1beta) and monocyte chemoattractant 
      protein-1 (MCP-1) in the spleen at 4 and 24 h after ischemia onset. Furthermore, 
      reduced expression of Ly-6C and CCR2 coincided with a decrease in the number of 
      Ly-6C(high) MMs subset in the spleen at 4 h after ischemia onset. This suggests 
      alphaCD147 treatment abrogates cerebral ischemia-induced inflammatory activation of 
      splenic monocytes/macrophages (MMs). In addition, the experiment in 
      splenectomized mice showed the spleen as the major source of infiltrated 
      Ly-6C(high) MMs subset in the ischemic brain and that brain infiltration of 
      Ly-6C(high) MMs was reduced by alphaCD147 treatment. These results reveal CD147 as a 
      key mediator of the spleen's inflammatory activation in response to cerebral 
      ischemia.
FAU - Jin, Rong
AU  - Jin R
AD  - Department of Neurosurgery, Penn State Hershey Medical Center, 500 University 
      Drive, Hershey, PA, 17033, USA.
FAU - Zhong, Wei
AU  - Zhong W
AD  - Department of Neurosurgery, Penn State Hershey Medical Center, 500 University 
      Drive, Hershey, PA, 17033, USA.
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, Jiefang road 
      438, Zhenjiang, 212013, Jiangsu Province, China.
FAU - Liu, Shan
AU  - Liu S
AD  - Department of Neurosurgery, Penn State Hershey Medical Center, 500 University 
      Drive, Hershey, PA, 17033, USA.
FAU - Li, Guohong
AU  - Li G
AD  - Department of Neurosurgery, Penn State Hershey Medical Center, 500 University 
      Drive, Hershey, PA, 17033, USA. guohongli@pennstatehealth.psu.edu.
LA  - eng
GR  - R01 NS088719/NS/NINDS NIH HHS/United States
PT  - Journal Article
DEP - 20191030
PL  - England
TA  - J Neuroinflammation
JT  - Journal of neuroinflammation
JID - 101222974
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Bsg protein, mouse)
RN  - 136894-56-9 (Basigin)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/pharmacology/therapeutic use
MH  - Basigin/*antagonists & inhibitors/*physiology
MH  - Brain Ischemia/drug therapy/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Spleen/drug effects/*metabolism/pathology
MH  - Stroke/drug therapy/*metabolism
PMC - PMC6822438
OTO - NOTNLM
OT  - CD147
OT  - Cerebral ischemia
OT  - Cytokines
OT  - Inflammation
OT  - Monocytes/macrophages
OT  - Spleen
COIS- The authors declare that they have no competing interests.
EDAT- 2019/11/02 06:00
MHDA- 2020/03/31 06:00
PMCR- 2019/10/30
CRDT- 2019/11/01 06:00
PHST- 2019/04/03 00:00 [received]
PHST- 2019/10/01 00:00 [accepted]
PHST- 2019/11/01 06:00 [entrez]
PHST- 2019/11/02 06:00 [pubmed]
PHST- 2020/03/31 06:00 [medline]
PHST- 2019/10/30 00:00 [pmc-release]
AID - 10.1186/s12974-019-1609-y [pii]
AID - 1609 [pii]
AID - 10.1186/s12974-019-1609-y [doi]
PST - epublish
SO  - J Neuroinflammation. 2019 Oct 30;16(1):198. doi: 10.1186/s12974-019-1609-y.