PMID- 31667207
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231014
IS  - 2352-3204 (Electronic)
IS  - 2352-3204 (Linking)
VI  - 11
DP  - 2019 Dec
TI  - Long non-coding RNA ANRIL promotes proliferation, clonogenicity, invasion and 
      migration of laryngeal squamous cell carcinoma by regulating miR-181a/Snai2 axis.
PG  - 282-289
LID - 10.1016/j.reth.2019.07.007 [doi]
AB  - BACKGROUND: Laryngeal squamous cell carcinoma (LSCC) is the common cancer with 
      poor prognosis. Long non-coding RNA (lncRNA) ANRIL has been proven to play an 
      important role in many cancers. However up to now, the role of ANRIL in LSCC is 
      still poorly understood. The present study aimed to investigate the role and 
      underlying mechanisms of ANRIL and miR-181a in LSCC. METHODS: Expression of 
      ANRIL, miR-181a and Snai2 in both LSCC tissues and cells was determined by 
      qRT-PCR. CCK-8 assay, colony formation assay, flow cytometry analysis and 
      transwell assay were conducted to detect cell proliferation, clonogenicity, 
      apoptosis, invasion and migration, respectively. The binding between ANRIL and 
      miR-181a, as well miR-181a and Snai2 was confirmed by dual luciferase reporter 
      assay. Western blotting was used to determine the protein levels of Snail, Slug, 
      E-cadherin, N-cadherin and Vimentin. RESULTS: ANRIL was up-regulated while 
      miR-181a was down-regulated in LSCC tissues. ANRIL was negatively correlated with 
      miR-181a and was positively correlated with Snai1 and Snai2. Dual luciferase 
      reporter assay showed ANRIL could directly sponge miR-181a to counteract its 
      suppression on Snai2, serving as a positive regulator of Snai2. Either knockdown 
      of ANRIL or overexpression of miR-181a significantly inhibited the proliferation, 
      clonogenicity, invasion, migration and epithelial mesenchymal transformation 
      (EMT), as well as promoted the apoptosis of LSCC cells, and knockdown of miR-181a 
      reversed the effects. CONCLUSION: Inhibition of ANRIL could suppress cell 
      proliferation, clonogenicity, invasion and migration, as well as enhance cell 
      apoptosis of LSCC cells through regulation of miR-181a/Snai2 axis, indicating 
      that ANRIL might be a promising therapeutic target during the treatment of LSCC.
CI  - (c) 2019 The Japanese Society for Regenerative Medicine. Production and hosting by 
      Elsevier B.V.
FAU - Hao, Yan-Ru
AU  - Hao YR
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin 
      University, Changchun 130041, Jilin Province, PR China.
FAU - Zhang, De-Jun
AU  - Zhang DJ
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin 
      University, Changchun 130041, Jilin Province, PR China.
FAU - Fu, Ze-Ming
AU  - Fu ZM
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin 
      University, Changchun 130041, Jilin Province, PR China.
FAU - Guo, Ying-Yuan
AU  - Guo YY
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin 
      University, Changchun 130041, Jilin Province, PR China.
FAU - Guan, Guo-Fang
AU  - Guan GF
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin 
      University, Changchun 130041, Jilin Province, PR China.
LA  - eng
PT  - Journal Article
DEP - 20190920
PL  - Netherlands
TA  - Regen Ther
JT  - Regenerative therapy
JID - 101709085
PMC - PMC6813643
OTO - NOTNLM
OT  - EMT
OT  - Laryngeal squamous cell carcinoma
OT  - Snai2
OT  - lncRNA-ANRIL
OT  - miR-181a
EDAT- 2019/11/02 06:00
MHDA- 2019/11/02 06:01
PMCR- 2019/09/20
CRDT- 2019/11/01 06:00
PHST- 2019/01/31 00:00 [received]
PHST- 2019/07/10 00:00 [revised]
PHST- 2019/07/24 00:00 [accepted]
PHST- 2019/11/01 06:00 [entrez]
PHST- 2019/11/02 06:00 [pubmed]
PHST- 2019/11/02 06:01 [medline]
PHST- 2019/09/20 00:00 [pmc-release]
AID - S2352-3204(19)30015-X [pii]
AID - 10.1016/j.reth.2019.07.007 [doi]
PST - epublish
SO  - Regen Ther. 2019 Sep 20;11:282-289. doi: 10.1016/j.reth.2019.07.007. eCollection 
      2019 Dec.