PMID- 31667906
OWN - NLM
STAT- MEDLINE
DCOM- 20200430
LR  - 20211204
IS  - 1099-1573 (Electronic)
IS  - 0951-418X (Linking)
VI  - 34
IP  - 2
DP  - 2020 Feb
TI  - Bilobalide alleviates tumor necrosis factor-alpha-induced pancreatic beta-cell 
      MIN6 apoptosis and dysfunction through upregulation of miR-153.
PG  - 409-417
LID - 10.1002/ptr.6533 [doi]
AB  - Type 1 diabetes mellitus (T1DM) is a systemic disease and one classical type of 
      total DM. Bilobalide (BB) is constituted of EGb 761. Our purpose was identifying 
      the role of BB in TIDM in the current study. MIN6 cells were treated by TNF-alpha; 
      then, viability, apoptosis, and insulin secretion were assessed by performing 
      Cell Counting Kit-8 assay, flow cytometry, glucose-stimulated insulin secretion 
      assay, and western blot. The effects of BB were assessed to identify its 
      function. Further, the above mentioned parameters were reassessed when silencing 
      miR-153. TNF-alpha declined viability and insulin secretion as well as raised 
      apoptosis and inducible nitric oxide synthase (iNOS) expression in MIN6 cells. BB 
      alleviated the apoptosis and dysfunction induced by TNF-alpha. MiR-153 expression was 
      elevated by BB when induced by TNF-alpha. Increase of viability and insulin secretion 
      as well as decline of apoptosis and iNOS induced by BB treatment was alleviated 
      by silencing miR-153. The rates of p/t-p70S6K, p/t-mammalian target of rapamycin 
      (mTOR) and p/t-adenosine monophosphate-activated protein kinase (AMPK) were 
      raised by BB and suppressed by silencing miR-153 under TNF-alpha induced condition. 
      BB raised viability and insulin secretion, declined apoptosis and iNOS expression 
      by up-regulating miR-153. Furthermore, BB activated AMPK/mTOR pathway by 
      up-regulating miR-153.
CI  - (c) 2019 John Wiley & Sons, Ltd.
FAU - Hao, Yan
AU  - Hao Y
AD  - Department of Endocrinology, Jining No.1 People's Hospital, Jining, China.
FAU - Wang, Weiwei
AU  - Wang W
AD  - Department of Endocrinology, Jining No.1 People's Hospital, Jining, China.
FAU - Wu, Dong
AU  - Wu D
AD  - Emergency Department, Jining No.1 People's Hospital, Jining, China.
FAU - Liu, Kai
AU  - Liu K
AD  - Emergency Department, Jinxiang People's Hospital, Jining, China.
FAU - Sun, Yihan
AU  - Sun Y
AUID- ORCID: 0000-0001-6717-4898
AD  - Department of Endocrinology, Jining No.1 People's Hospital, Jining, China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20191031
PL  - England
TA  - Phytother Res
JT  - Phytotherapy research : PTR
JID - 8904486
RN  - 0 (Cyclopentanes)
RN  - 0 (Furans)
RN  - 0 (Ginkgolides)
RN  - 0 (Insulin)
RN  - 0 (MIRN153 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, mouse)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - M81D2O8H7U (bilobalide)
SB  - IM
RIN - Phytother Res. 2020 Oct;34(10):2778. PMID: 33051935
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Cell Line, Tumor
MH  - Cyclopentanes/*pharmacology
MH  - Furans/*pharmacology
MH  - Ginkgolides/*pharmacology
MH  - Insulin/*analysis
MH  - Mice
MH  - MicroRNAs/*metabolism
MH  - Nitric Oxide Synthase Type II/metabolism
MH  - Ribosomal Protein S6 Kinases, 70-kDa/metabolism
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Transcriptional Activation
MH  - Tumor Necrosis Factor-alpha/adverse effects
MH  - Up-Regulation
OTO - NOTNLM
OT  - AMPK/mTOR pathway
OT  - apoptosis
OT  - diabetes mellitus (DM)
OT  - dysfunction
EDAT- 2019/11/02 06:00
MHDA- 2020/05/01 06:00
CRDT- 2019/11/01 06:00
PHST- 2019/05/14 00:00 [received]
PHST- 2019/09/22 00:00 [revised]
PHST- 2019/10/09 00:00 [accepted]
PHST- 2019/11/02 06:00 [pubmed]
PHST- 2020/05/01 06:00 [medline]
PHST- 2019/11/01 06:00 [entrez]
AID - 10.1002/ptr.6533 [doi]
PST - ppublish
SO  - Phytother Res. 2020 Feb;34(2):409-417. doi: 10.1002/ptr.6533. Epub 2019 Oct 31.