PMID- 31668066
OWN - NLM
STAT- MEDLINE
DCOM- 20191202
LR  - 20191202
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 67
IP  - 46
DP  - 2019 Nov 20
TI  - Crystal Structure Analysis and Conformational Epitope Mutation of Triosephosphate 
      Isomerase, a Mud Crab Allergen.
PG  - 12918-12926
LID - 10.1021/acs.jafc.9b05279 [doi]
AB  - The triosephosphate isomerase (TIM), Scy p 8, is a crab allergen and shows 
      cross-reactivity in the shellfish. Here, recombinant Scy p 8 was expressed, and 
      its crystal structure was determined at a resolution of 1.8 A. The 
      three-dimensional structure of Scy p 8 is primarily composed of a (beta/alpha)(8)-barrel 
      motif prototype. Additionally, Scy p 8 showed cross-reactivity with high 
      sequential and secondary structural identity among TIMs from shellfish species. 
      The site-directed mutagenesis of critical amino acids of conformational epitopes 
      was carried out, and the mutants of Trp 168 and Lys 237 to Ala reduced 
      immunoglobulin E (IgE)-binding activity by approximately 30%, compared with 
      wild-type TIM in an inhibition ELISA; however, it still induced basophil 
      activation despite the interpatient variability between patients. These results 
      can help to provide an accurate template for the analysis of the IgE binding and 
      establish meaningful relationships between structure and allergenicity.
FAU - Xia, Fei
AU  - Xia F
AD  - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine 
      Functional Food, Fujian Provincial Engineering Technology Research Center of 
      Marine Functional Food, Fujian Collaborative Innovation Center for Exploitation 
      and Utilization of Marine Biological Resources , Jimei University , Xiamen , 
      Fujian 361021 , China.
FAU - Li, Meng-Si
AU  - Li MS
AD  - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine 
      Functional Food, Fujian Provincial Engineering Technology Research Center of 
      Marine Functional Food, Fujian Collaborative Innovation Center for Exploitation 
      and Utilization of Marine Biological Resources , Jimei University , Xiamen , 
      Fujian 361021 , China.
FAU - Liu, Qing-Mei
AU  - Liu QM
AD  - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine 
      Functional Food, Fujian Provincial Engineering Technology Research Center of 
      Marine Functional Food, Fujian Collaborative Innovation Center for Exploitation 
      and Utilization of Marine Biological Resources , Jimei University , Xiamen , 
      Fujian 361021 , China.
FAU - Liu, Meng
AU  - Liu M
AD  - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine 
      Functional Food, Fujian Provincial Engineering Technology Research Center of 
      Marine Functional Food, Fujian Collaborative Innovation Center for Exploitation 
      and Utilization of Marine Biological Resources , Jimei University , Xiamen , 
      Fujian 361021 , China.
FAU - Yang, Yang
AU  - Yang Y
AD  - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine 
      Functional Food, Fujian Provincial Engineering Technology Research Center of 
      Marine Functional Food, Fujian Collaborative Innovation Center for Exploitation 
      and Utilization of Marine Biological Resources , Jimei University , Xiamen , 
      Fujian 361021 , China.
FAU - Cao, Min-Jie
AU  - Cao MJ
AD  - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine 
      Functional Food, Fujian Provincial Engineering Technology Research Center of 
      Marine Functional Food, Fujian Collaborative Innovation Center for Exploitation 
      and Utilization of Marine Biological Resources , Jimei University , Xiamen , 
      Fujian 361021 , China.
FAU - Chen, Gui-Xia
AU  - Chen GX
AD  - Women and Children's Hospital Affiliated to Xiamen University , Xiamen , Fujian 
      361003 , China.
FAU - Jin, Tengchuan
AU  - Jin T
AUID- ORCID: 0000-0002-1395-188X
AD  - Hefei National Laboratory for Physical Sciences at Microscale, CAS Key Laboratory 
      of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine , 
      University of Science & Technology of China , Hefei , Anhui 230027 , China.
FAU - Liu, Guang-Ming
AU  - Liu GM
AUID- ORCID: 0000-0002-8689-0504
AD  - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine 
      Functional Food, Fujian Provincial Engineering Technology Research Center of 
      Marine Functional Food, Fujian Collaborative Innovation Center for Exploitation 
      and Utilization of Marine Biological Resources , Jimei University , Xiamen , 
      Fujian 361021 , China.
LA  - eng
PT  - Journal Article
DEP - 20191111
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Epitopes)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 5.3.1.1 (Triose-Phosphate Isomerase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brachyura/chemistry/*enzymology/genetics/immunology
MH  - Cross Reactions
MH  - Crystallization
MH  - Epitopes/*chemistry/genetics/immunology
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Molecular Conformation
MH  - Protein Conformation
MH  - Sequence Alignment
MH  - Shellfish/analysis
MH  - Shellfish Hypersensitivity/immunology
MH  - Triose-Phosphate Isomerase/chemistry/genetics/*immunology
OTO - NOTNLM
OT  - crab allergen
OT  - cross-reactivity
OT  - crystal structure
OT  - site directed mutagenesis
OT  - triosephosphate isomerase
EDAT- 2019/11/02 06:00
MHDA- 2019/12/04 06:00
CRDT- 2019/11/01 06:00
PHST- 2019/11/02 06:00 [pubmed]
PHST- 2019/12/04 06:00 [medline]
PHST- 2019/11/01 06:00 [entrez]
AID - 10.1021/acs.jafc.9b05279 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2019 Nov 20;67(46):12918-12926. doi: 10.1021/acs.jafc.9b05279. 
      Epub 2019 Nov 11.