PMID- 31668928 OWN - NLM STAT- MEDLINE DCOM- 20201102 LR - 20211030 IS - 1873-5894 (Electronic) IS - 0730-725X (Print) IS - 0730-725X (Linking) VI - 66 DP - 2020 Feb TI - Tagged cine magnetic resonance imaging to quantify regional mechanical changes after acute myocardial infarction. PG - 208-218 LID - S0730-725X(19)30245-0 [pii] LID - 10.1016/j.mri.2019.09.010 [doi] AB - PURPOSE: The conventional volumetric approaches of measuring cardiac function are load-dependent, and are not able to discriminate functional changes in the infarct, transition and remote myocardium. We examined phase-dependent regional mechanical changes in the infarct, transition and remote regions after acute myocardial infarction (MI) in a preclinical mouse model using cardiovascular magnetic resonance imaging (CMR). METHODS: We induced acute MI in six mice with left anterior descending coronary artery ligation. We then examined cardiac (infarct, transition and remote-zone) morphology and function utilizing 9.4 T high field CMR before and 2 weeks after the induction of acute MI. Myocardial scar tissue was evaluated by using CMR with late gadolinium enhancement (LGE). After determining global function through volumetric analysis, regional wall motion was evaluated by measuring wall thickening and radial velocities. Strain rate imaging was performed to assess circumferential contraction and relaxation at the myocardium, endocardium, and epicardium. RESULTS: There was abnormal LGE in the anterior walls after acute MI suggesting a successful MI procedure. The transition zone consisted of a mixed signal intensity, while the remote zone contained viable myocardium. As expected, the infarct zone had demonstrated severely decreased myocardial velocities and strain rates, suggesting reduced contraction and relaxation function. Compared to pre-infarct baseline, systolic and diastolic velocities (v(S) and v(D)) were significantly reduced at the transition zone (v(S): -1.86 +/- 0.16 cm/s vs -0.68 +/- 0.13 cm/s, P < 0.001; v(D): 1.86 +/- 0.17 cm/s vs 0.53 +/- 0.06 cm/s, P < 0.001) and remote zone (v(S): -1.86 +/- 0.16 cm/s vs -0.65 +/- 0.12 cm/s, P < 0.001; v(D): 1.86 +/- 0.16 cm/s vs 0.51 +/- 0.04 cm/s, P < 0.001). Myocardial peak systolic and diastolic strain rates (SR(S) and SR(D)) were significantly lower in the transition zone (SR(S): -4.2 +/- 0.3 s(-1) vs -1.3 +/- 0.2 s(-1), P < 0.001; SR(D): 3.9 +/- 0.3 s(-1) vs 1.3 +/- 0.2 s(-1), P < 0.001) and remote zone (SR(S): -3.8 +/- 0.3 s(-1) vs -1.4 +/- 0.3 s(-1), P < 0.001; SR(D): 3.5 +/- 0.2 s(-1) vs 1.5 +/- 0.4 s(-1), P = 0.006). Endocardial and epicardial SR(S) and SR(D) were similarly reduced in the transition and remote zones compared to baseline. CONCLUSIONS: This study, for the first time, utilized state-of-the art high-field CMR algorithms in a preclinical mouse model for a comprehensive and controlled evaluation of the regional mechanical changes in the transition and remote zones, after acute MI. Our data demonstrate that CMR can quantitatively monitor dynamic post-MI remodeling in the transition and remote zones, thereby serving as a gold standard tool for therapeutic surveillance. CI - Copyright (c) 2019 Elsevier Inc. All rights reserved. FAU - Karthikeyan, Badri AU - Karthikeyan B AD - Department of Medicine, Division of Cardiology, Jacob's School of Medicine and Biomedical Sciences, Buffalo, NY, United States of America. FAU - Sonkawade, Swati D AU - Sonkawade SD AD - Department of Medicine, Division of Cardiology, Jacob's School of Medicine and Biomedical Sciences, Buffalo, NY, United States of America. FAU - Pokharel, Saraswati AU - Pokharel S AD - Department of Pathology and Laboratory Medicine, Roswell Park Comprehensive Cancer Center, Buffalo, NY, United States of America. FAU - Preda, Marilena AU - Preda M AD - Center for Biomedical Imaging at the Clinical and Translational Science Institute, University at Buffalo, Buffalo, NY, United States of America. FAU - Schweser, Ferdinand AU - Schweser F AD - Center for Biomedical Imaging at the Clinical and Translational Science Institute, University at Buffalo, Buffalo, NY, United States of America. FAU - Zivadinov, Robert AU - Zivadinov R AD - Center for Biomedical Imaging at the Clinical and Translational Science Institute, University at Buffalo, Buffalo, NY, United States of America. FAU - Kim, Minhyung AU - Kim M AD - Department of Surgical Oncology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, United States of America. FAU - Sharma, Umesh C AU - Sharma UC AD - Department of Medicine, Division of Cardiology, Jacob's School of Medicine and Biomedical Sciences, Buffalo, NY, United States of America. Electronic address: sharmau@buffalo.edu. LA - eng GR - K08 HL131987/HL/NHLBI NIH HHS/United States GR - KL2 TR001413/TR/NCATS NIH HHS/United States GR - R21 HL138555/HL/NHLBI NIH HHS/United States GR - UL1 TR001412/TR/NCATS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20191024 PL - Netherlands TA - Magn Reson Imaging JT - Magnetic resonance imaging JID - 8214883 SB - IM MH - Acute Disease MH - Animals MH - Disease Models, Animal MH - Heart/diagnostic imaging/physiopathology MH - Magnetic Resonance Imaging, Cine/*methods MH - Mice MH - Myocardial Infarction/*diagnostic imaging/pathology/*physiopathology MH - Myocardium/pathology PMC - PMC7031039 MID - NIHMS1062548 OTO - NOTNLM OT - Cardiovascular magnetic resonance imaging OT - Left ventricular remodeling OT - Myocardial infarction OT - Strain imaging COIS- Declaration of competing interest None. EDAT- 2019/11/02 06:00 MHDA- 2020/11/03 06:00 PMCR- 2021/02/01 CRDT- 2019/11/01 06:00 PHST- 2019/04/17 00:00 [received] PHST- 2019/08/16 00:00 [revised] PHST- 2019/09/15 00:00 [accepted] PHST- 2019/11/02 06:00 [pubmed] PHST- 2020/11/03 06:00 [medline] PHST- 2019/11/01 06:00 [entrez] PHST- 2021/02/01 00:00 [pmc-release] AID - S0730-725X(19)30245-0 [pii] AID - 10.1016/j.mri.2019.09.010 [doi] PST - ppublish SO - Magn Reson Imaging. 2020 Feb;66:208-218. doi: 10.1016/j.mri.2019.09.010. Epub 2019 Oct 24.