PMID- 31669164
OWN - NLM
STAT- MEDLINE
DCOM- 20200608
LR  - 20200608
IS  - 2095-4964 (Print)
VI  - 17
IP  - 6
DP  - 2019 Nov
TI  - Induction of sub-G(0) arrest and apoptosis by seed extract of Moringa peregrina 
      (Forssk.) Fiori in cervical and prostate cancer cell lines.
PG  - 410-422
LID - S2095-4964(19)30097-4 [pii]
LID - 10.1016/j.joim.2019.09.004 [doi]
AB  - OBJECTIVE: This study investigated cytotoxicity and induction of apoptosis in 
      human cervical cancer cells (HELA) and prostate cancer cells (PC-3) using the 
      most active fraction of Moringa peregrina seed extract. METHODS: Dried and 
      powdered seeds were extracted using 95% ethanol. The total ethanolic extract was 
      further dissolved in distilled water and separated into petroleum ether, 
      chloroform, ethyl acetate and aqueous extracts. Based on the results of in vitro 
      anticancer studies of all extracts, the most highly active extract was selected 
      for evaluation of apoptosis induction and cell cycle analysis on HELA and PC-3 
      cells at its half maximal inhibitory concentration using flow cytometry; DNA 
      fragmentation by agarose gel electrophoresis and the expression of protein were 
      measured by Western blot. RESULTS: The chloroform fraction from the ethanolic 
      extract of M. peregrina (CFEE) was the most active antitumor fraction. The 
      selectivity index, determined using the normal Vero cell line, indicated that 
      CFEE had a high degree of selectivity against HELA and PC-3 cells. CFEE induced 
      apoptosis, confirmed by cell cycle arrest at sub-G(0) phase and DNA 
      fragmentation. CFEE induced an increase in mRNA expression of caspase-3, a 
      decrease in Bcl-2 mRNA expression, and decreased ATP levels. CFEE increased 
      protein expression of caspase-3 and decreased protein expression of 
      poly-ADP-ribose polymerase-1 (PARP-1). Flow cytometric analysis showed an 
      appreciable increase in the number of cells in the early apoptotic stage in 
      CFEE-treated HELA and PC-3 cells. CFEE treatment significantly increased lipid 
      peroxidation (malondialdehyde level) in HELA and PC-3 cells. CONCLUSION: Seed 
      extract of M. peregrina displayed a significant antitumor effect through 
      apoptosis induction in HELA and PC-3 cells.
CI  - Copyright (c) 2019 Shanghai Changhai Hospital. Published by Elsevier B.V. All 
      rights reserved.
FAU - Abou-Hashem, Maged Mohamed Maher
AU  - Abou-Hashem MMM
AD  - Faculty of Pharmacy, Pharmacognosy Department, Zagazig University, Zagazig 44511, 
      Egypt.
FAU - Abo-Elmatty, Dina Mohamed
AU  - Abo-Elmatty DM
AD  - Faculty of Pharmacy, Biochemistry Department, Suez Canal University, Ismailia 
      41522, Egypt.
FAU - Mesbah, Noha Mostafa
AU  - Mesbah NM
AD  - Faculty of Pharmacy, Biochemistry Department, Suez Canal University, Ismailia 
      41522, Egypt.
FAU - Abd El-Mawgoud, Ahmed Mohamed
AU  - Abd El-Mawgoud AM
AD  - Egyptian International Pharmaceuticals Industries Company, Tenth of Ramadan City 
      44629, Egypt. Electronic address: awaditech@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20190930
PL  - Netherlands
TA  - J Integr Med
JT  - Journal of integrative medicine
JID - 101603118
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Antineoplastic Agents, Phytogenic/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Blotting, Western
MH  - Cell Cycle Checkpoints/*drug effects
MH  - Chromatography, Liquid
MH  - Egypt
MH  - Gas Chromatography-Mass Spectrometry
MH  - HeLa Cells
MH  - Humans
MH  - Moringa/*chemistry
MH  - PC-3 Cells
MH  - Plant Extracts/*pharmacology
MH  - Seeds/chemistry
OTO - NOTNLM
OT  - Antiproliferation
OT  - Apoptosis
OT  - Fractionation
OT  - Moringa peregrina
EDAT- 2019/11/02 06:00
MHDA- 2020/06/09 06:00
CRDT- 2019/11/01 06:00
PHST- 2019/03/11 00:00 [received]
PHST- 2019/05/20 00:00 [accepted]
PHST- 2019/11/02 06:00 [pubmed]
PHST- 2020/06/09 06:00 [medline]
PHST- 2019/11/01 06:00 [entrez]
AID - S2095-4964(19)30097-4 [pii]
AID - 10.1016/j.joim.2019.09.004 [doi]
PST - ppublish
SO  - J Integr Med. 2019 Nov;17(6):410-422. doi: 10.1016/j.joim.2019.09.004. Epub 2019 
      Sep 30.