PMID- 31669458
OWN - NLM
STAT- MEDLINE
DCOM- 20210205
LR  - 20210205
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 275
DP  - 2020 Jan 2
TI  - Activation of sialoadhesin down-regulates the levels of innate antiviral 
      cytokines in porcine alveolar macrophages in vitro.
PG  - 197792
LID - S0168-1702(19)30503-9 [pii]
LID - 10.1016/j.virusres.2019.197792 [doi]
AB  - Porcine sialoadhesin (pSn) is a crucial porcine reproductive and respiratory 
      syndrome virus (PRRSV) receptor mediating the attachment and internalization of 
      virus into its major target cells, porcine alveolar macrophages (PAMs). However, 
      the role of pSn in innate antiviral immune response has not yet been 
      investigated. In this study, our results showed that PRRSV down-regulated 
      significantly the mRNA levels of IFN-alpha, IFN-beta, IFN-gamma, IFN-lambda1, IFN-lambda3 and IFN-lambda4 
      and up-regulated significantly the mRNA levels of IL-10 and pSn in infected PAMs 
      in vitro, suggesting that PRRSV infection inhibited the transcription of innate 
      antiviral cytokines in host cells. Our results also showed that selective 
      activation of pSn down-regulated significantly the mRNA levels of IFN-alpha, IFN-beta, 
      IFN-gamma, IFN-lambda1, IFN-lambda3, IFN-lambda4 and TNF-alpha and up-regulated significantly the mRNA 
      level of IL-10 in PAMs in vitro, suggesting that pSn signaling inhibited the 
      transcription of innate antiviral cytokines. Further results showed that pSn1, 
      pSn2, pSn3, pSn4 and pSn5 domains of pSn were responsible for the inhibition of 
      levels of innate antiviral cytokines. In conclusion, our results suggested that 
      pSn suppressed innate antiviral immune response by down-regulating the levels of 
      innate antiviral cytokines in PAMs. It was possible that PRRSV-pSn interaction 
      may suppress innate antiviral immune response to PRRSV infection by repressing 
      the production of innate antiviral cytokines.
CI  - Copyright (c) 2019. Published by Elsevier B.V.
FAU - Zhang, Liujun
AU  - Zhang L
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      No. 95 Wen Hua Road, Zhengzhou, 450002, China.
FAU - Xu, Ruiqin
AU  - Xu R
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      No. 95 Wen Hua Road, Zhengzhou, 450002, China.
FAU - Wei, Fengling
AU  - Wei F
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      No. 95 Wen Hua Road, Zhengzhou, 450002, China.
FAU - Li, Wen
AU  - Li W
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      No. 95 Wen Hua Road, Zhengzhou, 450002, China.
FAU - Li, Xiangtong
AU  - Li X
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      No. 95 Wen Hua Road, Zhengzhou, 450002, China.
FAU - Zhang, Gaiping
AU  - Zhang G
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      No. 95 Wen Hua Road, Zhengzhou, 450002, China. Electronic address: 
      zhanggaiping2003@163.com.
FAU - Xia, Pingan
AU  - Xia P
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      No. 95 Wen Hua Road, Zhengzhou, 450002, China. Electronic address: xpa88@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191024
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Antiviral Agents)
RN  - 0 (Cytokines)
RN  - 0 (Sialic Acid Binding Ig-like Lectin 1)
SB  - IM
MH  - Animals
MH  - Antiviral Agents/immunology
MH  - Cells, Cultured
MH  - Cytokines/*immunology
MH  - Down-Regulation
MH  - *Immunity, Innate
MH  - Macrophages, Alveolar/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus
MH  - Sialic Acid Binding Ig-like Lectin 1/*immunology
MH  - Specific Pathogen-Free Organisms
MH  - Swine
OTO - NOTNLM
OT  - Antiviral cytokines
OT  - CD169
OT  - PAMs
OT  - Sialoadhesin
OT  - Siglec1
EDAT- 2019/11/02 06:00
MHDA- 2021/02/07 06:00
CRDT- 2019/11/01 06:00
PHST- 2019/07/18 00:00 [received]
PHST- 2019/10/19 00:00 [revised]
PHST- 2019/10/20 00:00 [accepted]
PHST- 2019/11/02 06:00 [pubmed]
PHST- 2021/02/07 06:00 [medline]
PHST- 2019/11/01 06:00 [entrez]
AID - S0168-1702(19)30503-9 [pii]
AID - 10.1016/j.virusres.2019.197792 [doi]
PST - ppublish
SO  - Virus Res. 2020 Jan 2;275:197792. doi: 10.1016/j.virusres.2019.197792. Epub 2019 
      Oct 24.