PMID- 31669634
OWN - NLM
STAT- MEDLINE
DCOM- 20200127
LR  - 20200127
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1130-1131
DP  - 2019 Nov 1
TI  - Determination of anandamide in cerebrospinal fluid samples by disposable pipette 
      extraction and ultra-high performance liquid chromatography tandem mass 
      spectrometry.
PG  - 121809
LID - S1570-0232(19)30687-7 [pii]
LID - 10.1016/j.jchromb.2019.121809 [doi]
AB  - This work describes the development and validation of an ultra-high performance 
      liquid chromatography tandem mass spectrometry method that uses disposable 
      pipette extraction (DPX-UHPLC-MS/MS) to determine the endocannabinoid anandamide 
      (AEA) in cerebrospinal fluid samples (CSF). The DPX parameters sorption 
      equilibrium time, sample volume, number of draw-eject cycles, washing solvent 
      volume, and elution solvent volume were optimized by design of experiments (DOE) 
      techniques. The simple DPX protocol proposed herein required a reduced amount of 
      CSF sample and organic solvent. The DPX-UHPLC-MS/MS method presented linear range 
      from 0.10 ng mL(-1) (LLOQ) to 3.0 ng mL(-1), inter- and intra-assay accuracy with 
      EPR values varying from -8.2% to 9.6%, inter- and intra-assay precision with CV 
      values ranging from 1.3% to 14.8% (except for the LLOQ), and no significant 
      matrix effect. The innovative DPX-UHPLC-MS/MS method was successfully applied to 
      determine AEA in CSF samples from Parkinson's disease (PD) patients and should 
      therefore be used in clinical studies.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Oliveira, Igor Gustavo de Carvalho
AU  - Oliveira IGC
AD  - Universidade de Sao Paulo (USP), Faculdade de Filosofia, Ciencias e Letras de 
      Ribeirao Preto, Departamento de Quimica, Avenida Bandeirantes, 3900, CEP 
      14040-901 Ribeirao Preto, SP, Brazil.
FAU - Marchioni, Camila
AU  - Marchioni C
AD  - Universidade de Sao Paulo (USP), Faculdade de Ciencias Farmaceuticas de Ribeirao 
      Preto, Avenida Bandeirantes, 3900, CEP 14040-901 Ribeirao Preto, SP, Brazil.
FAU - Queiroz, Maria Eugenia Costa
AU  - Queiroz MEC
AD  - Universidade de Sao Paulo (USP), Faculdade de Filosofia, Ciencias e Letras de 
      Ribeirao Preto, Departamento de Quimica, Avenida Bandeirantes, 3900, CEP 
      14040-901 Ribeirao Preto, SP, Brazil; Universidade de Sao Paulo (USP), Faculdade 
      de Ciencias Farmaceuticas de Ribeirao Preto, Avenida Bandeirantes, 3900, CEP 
      14040-901 Ribeirao Preto, SP, Brazil. Electronic address: mariaeqn@ffclrp.usp.br.
LA  - eng
PT  - Journal Article
DEP - 20191004
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Arachidonic Acids)
RN  - 0 (Endocannabinoids)
RN  - 0 (Polyunsaturated Alkamides)
RN  - UR5G69TJKH (anandamide)
SB  - IM
MH  - Arachidonic Acids/*cerebrospinal fluid/isolation & purification
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Endocannabinoids/*cerebrospinal fluid/isolation & purification
MH  - Humans
MH  - Linear Models
MH  - Polyunsaturated Alkamides/*cerebrospinal fluid/isolation & purification
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Anandamide
OT  - Cerebrospinal fluid samples
OT  - Disposable pipette extraction
OT  - Ultra-high performance liquid chromatography-tandem mass spectrometry
EDAT- 2019/11/02 06:00
MHDA- 2020/01/28 06:00
CRDT- 2019/11/01 06:00
PHST- 2019/05/14 00:00 [received]
PHST- 2019/09/13 00:00 [revised]
PHST- 2019/09/14 00:00 [accepted]
PHST- 2019/11/02 06:00 [pubmed]
PHST- 2020/01/28 06:00 [medline]
PHST- 2019/11/01 06:00 [entrez]
AID - S1570-0232(19)30687-7 [pii]
AID - 10.1016/j.jchromb.2019.121809 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Nov 1;1130-1131:121809. doi: 
      10.1016/j.jchromb.2019.121809. Epub 2019 Oct 4.