PMID- 31669889
OWN - NLM
STAT- MEDLINE
DCOM- 20200417
LR  - 20200417
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 77
DP  - 2019 Dec
TI  - Protostemonine alleviates heat-killed methicillin-resistant Staphylococcus 
      aureus-induced acute lung injury through MAPK and NF-kappaB signaling pathways.
PG  - 105964
LID - S1567-5769(19)31836-3 [pii]
LID - 10.1016/j.intimp.2019.105964 [doi]
AB  - Acute lung injury (ALI) and its most severe form acute respiratory distress 
      syndrome (ARDS) caused by gram-positive bacteria threatens human life because 
      effective treatments and medicines is unavailable. Protostemonine (PSN), an 
      active alkaloid mainly isolated from the roots of Stemona sesslifolia, has 
      anti-inflammatory effects on asthma and gram-negative bacteria-induced ALI. Here, 
      we found that PSN exhibits anti-inflammatory effects and alleviates heat-killed 
      methicillin-resistant Staphylococcus aureus (HKMRSA)-induced pneumonia. PSN 
      treatment significantly attenuated HKMRSA-induced pathological injury, pulmonary 
      neutrophil infiltration, tissue permeability and the production of 
      pro-inflammatory cytokines (TNF-alpha, IL-1beta and IL-6) in murine ALI model. In 
      addition, PSN decreased the content of TNF-alpha, IL-1beta, IL-6 and the expression of 
      iNOS, as well as the production of NO in HKMRSA-induced bone marrow derived 
      macrophages (BMDMs). Furthermore, treatment with PSN suppressed the activation of 
      MAPKs (e.g. p38 MAPK, JNK and ERK) and NF-kappaB. Collectively, our results suggest 
      that PSN ameliorates gram-positive bacteria-induced ALI in mice by inhibition of 
      the MAPK and NF-kappaB signaling pathways, and our studies suggest that PSN might be 
      a novel candidate for treating ALI/ARDS.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Wu, Yaxian
AU  - Wu Y
AD  - Wuxi School of Medicine, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, PR 
      China.
FAU - Nie, Yunjuan
AU  - Nie Y
AD  - Wuxi School of Medicine, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, PR 
      China.
FAU - Huang, Jianfeng
AU  - Huang J
AD  - Department of Radiation Oncology, Affiliated Hospital of Jiangnan University, 
      Wuxi 214062, PR China.
FAU - Qiu, Yubao
AU  - Qiu Y
AD  - Wuxi School of Medicine, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, PR 
      China.
FAU - Wan, Binbin
AU  - Wan B
AD  - Wuxi School of Medicine, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, PR 
      China.
FAU - Liu, Gang
AU  - Liu G
AD  - Wuxi School of Medicine, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, PR 
      China.
FAU - Chen, Junliang
AU  - Chen J
AD  - Wuxi School of Medicine, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, PR 
      China.
FAU - Chen, Dan
AU  - Chen D
AD  - Wuxi School of Medicine, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, PR 
      China; State Key Laboratory of Food Science and Technology, Jiangnan University, 
      Wuxi 214122, PR China. Electronic address: danchen09@163.com.
FAU - Pang, Qingfeng
AU  - Pang Q
AD  - Wuxi School of Medicine, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, PR 
      China. Electronic address: qfpang@jiangnan.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20191024
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (NF-kappa B)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Acute Lung Injury/*drug therapy/metabolism/microbiology
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Cytokines/metabolism
MH  - Hot Temperature
MH  - Inflammation/drug therapy/metabolism/microbiology
MH  - Lung/drug effects/metabolism/microbiology
MH  - Macrophages/drug effects/microbiology
MH  - Male
MH  - Methicillin-Resistant Staphylococcus aureus/*drug effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - NF-kappa B/*metabolism
MH  - Neutrophil Infiltration/drug effects
MH  - Pneumonia/drug therapy/metabolism/microbiology
MH  - Signal Transduction/*drug effects
MH  - Staphylococcal Infections/drug therapy/metabolism/microbiology
MH  - Stemonaceae/chemistry
OTO - NOTNLM
OT  - Acute lung injury
OT  - MAPK
OT  - MRSA
OT  - Macrophage
OT  - NF-kappaB
OT  - Protostemonine
EDAT- 2019/11/02 06:00
MHDA- 2020/04/18 06:00
CRDT- 2019/11/01 06:00
PHST- 2019/08/18 00:00 [received]
PHST- 2019/09/27 00:00 [revised]
PHST- 2019/10/03 00:00 [accepted]
PHST- 2019/11/02 06:00 [pubmed]
PHST- 2020/04/18 06:00 [medline]
PHST- 2019/11/01 06:00 [entrez]
AID - S1567-5769(19)31836-3 [pii]
AID - 10.1016/j.intimp.2019.105964 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2019 Dec;77:105964. doi: 10.1016/j.intimp.2019.105964. Epub 
      2019 Oct 24.