PMID- 31670280
OWN - NLM
STAT- MEDLINE
DCOM- 20191112
LR  - 20200108
IS  - 0304-4920 (Print)
IS  - 0304-4920 (Linking)
VI  - 62
IP  - 5
DP  - 2019 Sep-Oct
TI  - Action of citral on the substantia gelatinosa neurons of the trigeminal 
      subnucleus caudalis in juvenile mice.
PG  - 175-181
LID - 10.4103/CJP.CJP_32_19 [doi]
AB  - The substantia gelatinosa (SG) of the trigeminal subnucleus caudalis (Vc) is 
      admitted as a pivotal site of integrating and regulating orofacial nociceptive 
      inputs. Although citral (3,7-dimethyl-2,6-octadienal) is involved in 
      antinociception, the action mechanism of citral on the SG neurons of the Vc has 
      not been fully clarified yet. In this study, we examined the direct membrane 
      effects of citral and how citral mediates responses on the SG neurons of the Vc 
      in juvenile mice using a whole-cell patch-clamp technique. Under high chloride 
      pipette solution, citral showed repeatable inward currents that persisted in the 
      presence of tetrodotoxin, a voltage-gated Na(+) channel blocker, and 
      6-cyano-7-nitro-quinoxaline-2,3-dione, a non-N-methyl-D-aspartate (NMDA) 
      glutamate receptor antagonist, D-2-amino-5-phosphonopentanoic acid, an NMDA 
      receptor antagonist. However, the citral-induced inward currents were partially 
      blocked by picrotoxin, a gamma-aminobutyric acid (GABA(A))-receptor antagonist, 
      or by strychnine, a glycine receptor antagonist. Further, the citral-induced 
      responses were almost blocked by picrotoxin with strychnine. We also found that 
      citral exhibited additive effect with GABA-induced inward currents and 
      glycine-induced inward currents were potentiated by citral. In addition, citral 
      suppressed the firing activities by positive current injection on the SG neurons 
      of the Vc. Taken together, these results demonstrate that citral has glycine- 
      and/or GABA-mimetic actions and suggest that citral might be a potential target 
      for orofacial pain modulation by the activation of inhibitory neurotransmission 
      in the SG area of the Vc.
FAU - Nguyen, Thao Thi Phuong
AU  - Nguyen TTP
AD  - Department of Oral Physiology, School of Dentistry and Institute of Oral 
      Bioscience, Jeonbuk National University, Jeonju, Republic of Korea; Faculty of 
      Odonto - Stomatology, Hue University of Medicine and Pharmacy, Hue University, 
      Hue, Vietnam.
FAU - Jang, Seon Hui
AU  - Jang SH
AD  - Department of Oral Physiology, School of Dentistry and Institute of Oral 
      Bioscience, Jeonbuk National University, Jeonju, Republic of Korea.
FAU - Park, Soo Joung
AU  - Park SJ
AD  - Department of Oral Physiology, School of Dentistry and Institute of Oral 
      Bioscience, Jeonbuk National University, Jeonju, Republic of Korea.
FAU - Cho, Dong Hyu
AU  - Cho DH
AD  - Department of Obstetrics and Gynecology, Jeonbuk National University 
      Hospital-Jeonbuk, National University Medical School, Jeonju, Republic of Korea.
FAU - Han, Seong Kyu
AU  - Han SK
AD  - Department of Oral Physiology, School of Dentistry and Institute of Oral 
      Bioscience, Jeonbuk National University, Jeonju, Republic of Korea.
LA  - eng
PT  - Journal Article
PL  - India
TA  - Chin J Physiol
JT  - The Chinese journal of physiology
JID - 7804502
RN  - 0 (Acyclic Monoterpenes)
RN  - 0 (Monoterpenes)
RN  - T7EU0O9VPP (citral)
SB  - IM
EIN - Chin J Physiol. 2019 Nov-Dec;62(6):285. PMID: 31793466
MH  - Acyclic Monoterpenes
MH  - Animals
MH  - Mice
MH  - Monoterpenes
MH  - Neurons
MH  - Patch-Clamp Techniques
MH  - Rats, Sprague-Dawley
MH  - *Substantia Gelatinosa
OTO - NOTNLM
OT  - Citral
OT  - gamma-aminobutyric acid A receptor
OT  - glycine receptor
OT  - patch clamp
OT  - substantia gelatinosa neuron
COIS- None
EDAT- 2019/11/02 06:00
MHDA- 2019/11/13 06:00
CRDT- 2019/11/01 06:00
PHST- 2019/11/01 06:00 [entrez]
PHST- 2019/11/02 06:00 [pubmed]
PHST- 2019/11/13 06:00 [medline]
AID - ChinJPhysiol_2019_62_5_175_269833 [pii]
AID - 10.4103/CJP.CJP_32_19 [doi]
PST - ppublish
SO  - Chin J Physiol. 2019 Sep-Oct;62(5):175-181. doi: 10.4103/CJP.CJP_32_19.