PMID- 31670953 OWN - NLM STAT- MEDLINE DCOM- 20191202 LR - 20191210 IS - 1520-5118 (Electronic) IS - 0021-8561 (Linking) VI - 67 IP - 46 DP - 2019 Nov 20 TI - Enhancing the Sensitivity of the Bio-barcode Immunoassay for Triazophos Detection Based on Nanoparticles and Droplet Digital Polymerase Chain Reaction. PG - 12936-12944 LID - 10.1021/acs.jafc.9b05147 [doi] AB - An ultrasensitive bio-barcode competitive immunoassay method based on droplet digital polymerase chain reaction (ddPCR) was developed for the determination of triazophos. Gold nanoparticles (AuNPs) were coated with monoclonal antibodies (mAbs) and complementary double-stranded DNA (dsDNA), which included bio-barcode DNA and thiol-capped DNA. Magnetic nanoparticle (MNP) probes were constructed by modifying the MNPs with ovalbumin-hapten conjugates (OVA-hapten). The target pesticide and OVA-hapten on the surface of the MNP probes competed with the AuNP probes simultaneously, and then the bio-barcode DNA was released for quantification by ddPCR. The concentration of released DNA was inversely proportional to the concentration of pesticide to be tested. Under the optimum conditions, the competitive immunoassay exhibited a wide linear range of 0.01-20 ng/mL and a low detection limit of 0.002 ng/mL. Spike recovery tests were carried out using apple, rice, cabbage, and cucumber samples to verify the feasibility of the method. The recovery and relative standard deviations (RSDs) of the technique ranged from 76.9 to 94.4% and from 10.8 to 19.9%, respectively. To further validate the results, a linear correlation analysis was performed between the proposed method and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Consequently, the bio-barcode immunoassay based on nanoparticles and ddPCR, an ultrasensitive method, showed great potential for the determination of target pesticides in real samples. FAU - Cui, Xueyan AU - Cui X AD - Institute of Quality Standard and Testing Technology for Agro-Products , Chinese Academy of Agricultural Science , Beijing 100081 , People's Republic of China. FAU - Jin, Maojun AU - Jin M AD - Institute of Quality Standard and Testing Technology for Agro-Products , Chinese Academy of Agricultural Science , Beijing 100081 , People's Republic of China. FAU - Zhang, Chan AU - Zhang C AD - Institute of Quality Standard and Testing Technology for Agro-Products , Chinese Academy of Agricultural Science , Beijing 100081 , People's Republic of China. FAU - Du, Pengfei AU - Du P AD - Institute of Agro-Food Science and Technology , Shandong Academy of Agricultural Sciences , Jinan , Shandong 250100 , People's Republic of China. FAU - Chen, Ge AU - Chen G AD - Institute of Quality Standard and Testing Technology for Agro-Products , Chinese Academy of Agricultural Science , Beijing 100081 , People's Republic of China. FAU - Qin, Guoxin AU - Qin G AD - Agro-Product Quality Safety and Testing Technology Research Institute , Guangxi Academy of Agricultural Sciences , Nanning , Guangxi 530007 , People's Republic of China. FAU - Jiang, Zejun AU - Jiang Z AD - Institute of Quality Standard and Testing Technology for Agro-Products , Chinese Academy of Agricultural Science , Beijing 100081 , People's Republic of China. FAU - Zhang, Yudan AU - Zhang Y AD - Institute of Quality Standard and Testing Technology for Agro-Products , Chinese Academy of Agricultural Science , Beijing 100081 , People's Republic of China. FAU - Li, Mingjie AU - Li M AD - Institute of Quality Standard and Testing Technology for Agro-Products , Chinese Academy of Agricultural Science , Beijing 100081 , People's Republic of China. FAU - Liao, Yun AU - Liao Y AD - Institute of Quality Standard and Testing Technology for Agro-Products , Chinese Academy of Agricultural Science , Beijing 100081 , People's Republic of China. FAU - Wang, Yuanshang AU - Wang Y AD - Institute of Quality Standard and Testing Technology for Agro-Products , Chinese Academy of Agricultural Science , Beijing 100081 , People's Republic of China. FAU - Cao, Zhen AU - Cao Z AD - Institute of Quality Standard and Testing Technology for Agro-Products , Chinese Academy of Agricultural Science , Beijing 100081 , People's Republic of China. FAU - Yan, Feiyan AU - Yan F AD - Agro-Product Quality Safety and Testing Technology Research Institute , Guangxi Academy of Agricultural Sciences , Nanning , Guangxi 530007 , People's Republic of China. FAU - Abd El-Aty, A M AU - Abd El-Aty AM AD - Department of Pharmacology, Faculty of Veterinary Medicine , Cairo University , 12211 Giza , Egypt. AD - Department of Medical Pharmacology, Medical Faculty , Ataturk University , 25240 Erzurum , Turkey. FAU - Wang, Jing AU - Wang J AUID- ORCID: 0000-0002-8961-9277 AD - Institute of Quality Standard and Testing Technology for Agro-Products , Chinese Academy of Agricultural Science , Beijing 100081 , People's Republic of China. LA - eng PT - Evaluation Study PT - Journal Article DEP - 20191112 PL - United States TA - J Agric Food Chem JT - Journal of agricultural and food chemistry JID - 0374755 RN - 0 (Insecticides) RN - 0 (Organothiophosphates) RN - 0 (Triazoles) RN - 6099J8L0EE (triazophos) RN - 7440-57-5 (Gold) RN - 9007-49-2 (DNA) SB - IM MH - DNA/genetics MH - Gold/chemistry MH - Immunoassay/instrumentation/*methods MH - Insecticides/*analysis MH - Limit of Detection MH - Magnetics/methods MH - Metal Nanoparticles/chemistry MH - Organothiophosphates/*analysis MH - Polymerase Chain Reaction/*methods MH - Triazoles/*analysis OTO - NOTNLM OT - bio-barcode OT - ddPCR OT - gold nanoparticles OT - magnetic nanoparticles OT - triazophos EDAT- 2019/11/02 06:00 MHDA- 2019/12/04 06:00 CRDT- 2019/11/01 06:00 PHST- 2019/11/02 06:00 [pubmed] PHST- 2019/12/04 06:00 [medline] PHST- 2019/11/01 06:00 [entrez] AID - 10.1021/acs.jafc.9b05147 [doi] PST - ppublish SO - J Agric Food Chem. 2019 Nov 20;67(46):12936-12944. doi: 10.1021/acs.jafc.9b05147. Epub 2019 Nov 12.