PMID- 31672369
OWN - NLM
STAT- MEDLINE
DCOM- 20200302
LR  - 20211204
IS  - 1873-6424 (Electronic)
IS  - 0269-7491 (Linking)
VI  - 256
DP  - 2020 Jan
TI  - Microcystin-LR exposure decreased the fetal weight of mice by disturbance of 
      placental development and ROS-mediated endoplasmic reticulum stress in the 
      placenta.
PG  - 113362
LID - S0269-7491(19)32811-8 [pii]
LID - 10.1016/j.envpol.2019.113362 [doi]
AB  - The placenta is essential for sustaining the growth of the fetus. The aim of this 
      study was to investigate the role of the placenta in MCLR-induced significant 
      reduction in fetal weight, especially the changes in placental structure and 
      function. Pregnant mice were intraperitoneally injected with MCLR (5 or 20 mug/kg) 
      from gestational day (GD) 13 to GD17. The results showed MCLR reduced fetal 
      weight and placenta weight. The histological specimens of the placentas were 
      taken for light and electron microscopy studies. The internal space of blood 
      vessels decreased obviously in the placental labyrinth layer of mice treated with 
      MCLR. After the ultrastructural examination, the edema and intracytoplasmic 
      vacuolization, dilation of the endoplasmic reticulum and corrugation of the 
      nucleus were observed. In addition, maternal MCLR exposure caused a reduction of 
      11beta-hydroxysteroid dehydrogenase type 2 (HSD11B2) expression in placentae, a 
      critical regulator of fetal development. Several genes of placental growth 
      factors, such as Vegfalpha and Pgf and several genes of nutrient transport pumps, 
      such as Glut1 and Pcft were depressed in placentas of MCLR-treated mice, however 
      nutrient transporters Fatp1 and Snat4 were promoted. Moreover, significant 
      increases in malondialdehyde (MDA) revealed the occurrence of oxidative stress 
      caused by MCLR, which was also verified by remarkable decrease in the glutathione 
      levels, total antioxidant capacity (T-AOC) as well as the activity of antioxidant 
      enzymes. Real-time PCR and western blot analysis revealed that GRP78, CHOP, 
      XBP-1, peIF2alpha and pIRE1 were remarkable increased in placentas of MCLR-treated 
      mice, indicating that endoplasmic reticulum (ER) stress pathway was activated by 
      MCLR. Furthermore, oxidative stress and ER stress consequently triggered 
      apoptosis which contributed to the impairment of placental development. 
      Collectively, these results suggest maternal MCLR exposure results in reduced 
      fetal body weight, which might be associated with ROS-mediated endoplasmic 
      reticulum stress and impairment in placental structure and function.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Zhao, Sujuan
AU  - Zhao S
AD  - School of Public Health, Anhui Medical University, Hefei, 230032, China.
FAU - Zhong, Shengzheng
AU  - Zhong S
AD  - School of Public Health, Anhui Medical University, Hefei, 230032, China.
FAU - Wang, Fang
AU  - Wang F
AD  - School of Public Health, Anhui Medical University, Hefei, 230032, China.
FAU - Wang, Honghui
AU  - Wang H
AD  - School of Public Health, Anhui Medical University, Hefei, 230032, China.
FAU - Xu, Dexiang
AU  - Xu D
AD  - School of Public Health, Anhui Medical University, Hefei, 230032, China. 
      Electronic address: xudex@126.com.
FAU - Li, Guangyu
AU  - Li G
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China. 
      Electronic address: liguangyu@mail.hzau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20191011
PL  - England
TA  - Environ Pollut
JT  - Environmental pollution (Barking, Essex : 1987)
JID - 8804476
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (Hspa5 protein, mouse)
RN  - 0 (Marine Toxins)
RN  - 0 (Microcystins)
RN  - 0 (Reactive Oxygen Species)
RN  - EQ8332842Y (cyanoginosin LR)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - Endoplasmic Reticulum Stress/*drug effects
MH  - Female
MH  - Fetal Development/*drug effects
MH  - Fetal Weight/*drug effects
MH  - Gestational Age
MH  - Marine Toxins
MH  - Maternal Exposure/adverse effects
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Microcystins/*toxicity
MH  - Placenta/*drug effects/metabolism/pathology
MH  - Pregnancy
MH  - Reactive Oxygen Species/metabolism
OTO - NOTNLM
OT  - Endoplasmic reticulum stress
OT  - MCLR
OT  - Oxidative stress
OT  - Placental development
EDAT- 2019/11/02 06:00
MHDA- 2020/03/03 06:00
CRDT- 2019/11/02 06:00
PHST- 2019/07/12 00:00 [received]
PHST- 2019/09/23 00:00 [revised]
PHST- 2019/10/07 00:00 [accepted]
PHST- 2019/11/02 06:00 [pubmed]
PHST- 2020/03/03 06:00 [medline]
PHST- 2019/11/02 06:00 [entrez]
AID - S0269-7491(19)32811-8 [pii]
AID - 10.1016/j.envpol.2019.113362 [doi]
PST - ppublish
SO  - Environ Pollut. 2020 Jan;256:113362. doi: 10.1016/j.envpol.2019.113362. Epub 2019 
      Oct 11.