PMID- 31672459
OWN - NLM
STAT- MEDLINE
DCOM- 20200914
LR  - 20211204
IS  - 1873-6300 (Electronic)
IS  - 0891-0618 (Linking)
VI  - 102
DP  - 2019 Dec
TI  - Conditioned medium obtained from human amniotic mesenchymal stem cells attenuates 
      focal cerebral ischemia/reperfusion injury in rats by targeting mTOR pathway.
PG  - 101707
LID - S0891-0618(19)30118-8 [pii]
LID - 10.1016/j.jchemneu.2019.101707 [doi]
AB  - Conditioned medium obtained from human amniotic mesenchymal stem cells (hAMSC-CM) 
      was recently shown to have many antioxidant, antiapoptotic and proangiogenic 
      growth factors. The present study was performed to investigate whether protective 
      effects of hAMSC-CM against focal cerebral ischemia/ reperfusion (I/R) injury is 
      associated with modulation of the mammalian target of rapamycin (mTOR) pathway. A 
      rat model of middle cerebral artery occlusion (MCAO) was created and the animals 
      were divided into three groups including sham, MCAO and MCAO + hAMSC-CM. Drug was 
      administrated immediately after cerebral reperfusion (i.v). The expressions of 
      mTOR, p-mTOR and LC3 were measured using Western blotting and real time-PCR, 
      respectively. Apoptosis and neuronal loss were determined using TUNEL and Nissl 
      staining, respectively. Infarct volume and the blood-brain barrier (BBB) damage 
      were evaluated using 2,3,5-triphenyltetrazolium chloride (TTC) staining and Evans 
      Blue (EB) uptake, respectively. Compared with sham, significant infarct volume, 
      apoptotic cell death, and neuronal loss were found in MCAO rats that reversed by 
      hAMSC-CM (P < 0.05). Likewise, MCAO rats exhibited increased mRNA level of 
      light-chain 3 (LC3) and the LC3II/LC3I ratio as well as decreased expression 
      level of p-mTOR that reversed by hAMSC-CM (P < 0.05). There were no significant 
      differences in the expression of total mTOR among the experimental groups. In 
      summary, our results demonstrate that hAMSC-CM gives rise to neuroprotection 
      following ischemic stroke by restoring mTOR activity and inhibiting autophagy.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Nazarinia, Donya
AU  - Nazarinia D
AD  - Physiology Research Center and Department of Physiology, Faculty of Medicine, 
      Iran University of Medical Sciences, Tehran, Iran.
FAU - Aboutaleb, Nahid
AU  - Aboutaleb N
AD  - Physiology Research Center and Department of Physiology, Faculty of Medicine, 
      Iran University of Medical Sciences, Tehran, Iran. Electronic address: 
      Aboutaleb.n@iums.ac.ir.
FAU - Gholamzadeh, Raheleh
AU  - Gholamzadeh R
AD  - Physiology Research Center and Department of Physiology, Faculty of Medicine, 
      Iran University of Medical Sciences, Tehran, Iran.
FAU - Nasseri Maleki, Solmaz
AU  - Nasseri Maleki S
AD  - Physiology Research Center and Department of Physiology, Faculty of Medicine, 
      Iran University of Medical Sciences, Tehran, Iran.
FAU - Mokhtari, Behnaz
AU  - Mokhtari B
AD  - Physiology Research Center and Department of Physiology, Faculty of Medicine, 
      Iran University of Medical Sciences, Tehran, Iran.
FAU - Nikougoftar, Mahin
AU  - Nikougoftar M
AD  - Blood Transfusion Research Center, High Institute for Research and Education in 
      Transfusion Medicine, Tehran, Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191028
PL  - Netherlands
TA  - J Chem Neuroanat
JT  - Journal of chemical neuroanatomy
JID - 8902615
RN  - 0 (Culture Media, Conditioned)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Amnion/*cytology
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Blood-Brain Barrier/metabolism
MH  - Brain/metabolism
MH  - Brain Ischemia/*drug therapy/metabolism
MH  - Culture Media, Conditioned/*pharmacology
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/*drug therapy/metabolism
MH  - Signal Transduction/*drug effects
MH  - TOR Serine-Threonine Kinases/*metabolism
OTO - NOTNLM
OT  - Autophagy
OT  - Conditioned medium
OT  - Focal cerebral ischemia reperfusion injury
OT  - Mesenchymal stem cells
OT  - mTOR activity
EDAT- 2019/11/02 06:00
MHDA- 2020/09/15 06:00
CRDT- 2019/11/02 06:00
PHST- 2019/07/02 00:00 [received]
PHST- 2019/10/22 00:00 [revised]
PHST- 2019/10/23 00:00 [accepted]
PHST- 2019/11/02 06:00 [pubmed]
PHST- 2020/09/15 06:00 [medline]
PHST- 2019/11/02 06:00 [entrez]
AID - S0891-0618(19)30118-8 [pii]
AID - 10.1016/j.jchemneu.2019.101707 [doi]
PST - ppublish
SO  - J Chem Neuroanat. 2019 Dec;102:101707. doi: 10.1016/j.jchemneu.2019.101707. Epub 
      2019 Oct 28.