PMID- 31673996
OWN - NLM
STAT- MEDLINE
DCOM- 20201023
LR  - 20201023
IS  - 1573-5168 (Electronic)
IS  - 0920-1742 (Linking)
VI  - 46
IP  - 1
DP  - 2020 Feb
TI  - Brain transcriptome profile after CRISPR-induced ghrelin mutations in zebrafish.
PG  - 1-21
LID - 10.1007/s10695-019-00687-6 [doi]
AB  - Ghrelin (GRL) is a gut-brain hormone with a role in a wide variety of 
      physiological functions in mammals and fish, which points out the ghrelinergic 
      system as a key element for the appropriate biological functioning of the 
      organism. However, many aspects of the multifunctional nature of GRL remain to be 
      better explored, especially in fish. In this study, we used the CRISPR/Cas9 
      genome editing technique to generate F0 zebrafish in which the expression of grl 
      is compromised. Then, we employed high-throughput mRNA sequencing (RNA-seq) to 
      explore changes in the brain transcriptome landscape associated with the 
      silencing of grl. The CRISPR/Cas9 technique successfully edited the genome of F0 
      zebrafish resulting in individuals with considerably lower levels of GRL mRNAs 
      and protein and ghrelin O-acyl transferase (goat) mRNAs in the brain, intestine, 
      and liver compared to wild-type (WT) zebrafish. Analysis of brain transcriptome 
      revealed a total of 1360 differentially expressed genes (DEGs) between the grl 
      knockdown (KD) and WT zebrafish, with 664 up- and 696 downregulated DEGs in the 
      KD group. Functional enrichment analysis revealed that DEGs are highly enriched 
      for terms related to morphogenesis, metabolism (especially of lipids), 
      entrainment of circadian clocks, oxygen transport, apoptosis, and response to 
      stimulus. The present study offers valuable information on the central genes and 
      pathways implicated in functions of GRL, and points out the possible involvement 
      of this peptide in some novel functions in fish, such as apoptosis and oxygen 
      transport.
FAU - Blanco, Ayelen Melisa
AU  - Blanco AM
AD  - Departamento de Genetica, Fisiologia y Microbiologia, Facultad de Biologia, 
      Universidad Complutense de Madrid, Jose Antonio Novais 12, 28040, Madrid, Spain.
AD  - Laboratorio de Fisioloxia Animal, Departamento de Bioloxia Funcional e Ciencias 
      da Saude, Facultade de Bioloxia and Centro de Investigacion Marina, Universidade 
      de Vigo, Vigo, Spain.
FAU - Cortes, Raul
AU  - Cortes R
AD  - Departamento de Fisiologia de Peces y Biotecnologia, Instituto de Acuicultura 
      Torre de la Sal, Consejo Superior de Investigaciones Cientificas, Ribera de 
      Cabanes, 12595, Torre de la Sal, Castellon, Spain.
AD  - Centro de Investigacion en Recursos Naturales y Sustentabilidad, Universidad 
      Bernardo O'Higgins, Fabrica, 1990, Santiago, Chile.
FAU - Bertucci, Juan Ignacio
AU  - Bertucci JI
AD  - Toxicology Centre, University of Saskatchewan, Saskatoon, Canada.
FAU - Soletto, Lucia
AU  - Soletto L
AD  - Departamento de Fisiologia de Peces y Biotecnologia, Instituto de Acuicultura 
      Torre de la Sal, Consejo Superior de Investigaciones Cientificas, Ribera de 
      Cabanes, 12595, Torre de la Sal, Castellon, Spain.
FAU - Sanchez, Elisa
AU  - Sanchez E
AD  - Departamento de Fisiologia de Peces y Biotecnologia, Instituto de Acuicultura 
      Torre de la Sal, Consejo Superior de Investigaciones Cientificas, Ribera de 
      Cabanes, 12595, Torre de la Sal, Castellon, Spain.
FAU - Valenciano, Ana Isabel
AU  - Valenciano AI
AD  - Departamento de Genetica, Fisiologia y Microbiologia, Facultad de Biologia, 
      Universidad Complutense de Madrid, Jose Antonio Novais 12, 28040, Madrid, Spain.
FAU - Cerda-Reverter, Jose Miguel
AU  - Cerda-Reverter JM
AD  - Departamento de Fisiologia de Peces y Biotecnologia, Instituto de Acuicultura 
      Torre de la Sal, Consejo Superior de Investigaciones Cientificas, Ribera de 
      Cabanes, 12595, Torre de la Sal, Castellon, Spain. jm.cerda.reverter@csic.es.
FAU - Delgado, Maria Jesus
AU  - Delgado MJ
AUID- ORCID: 0000-0002-1185-9377
AD  - Departamento de Genetica, Fisiologia y Microbiologia, Facultad de Biologia, 
      Universidad Complutense de Madrid, Jose Antonio Novais 12, 28040, Madrid, Spain. 
      mjdelgad@bio.ucm.es.
LA  - eng
GR  - AGL2016-74857-C3-2-R/Ministry of Science, Innovation and Universities/
GR  - AGL2016-74857-C3-3-R/Ministry of Science, Innovation and Universities/
PT  - Journal Article
DEP - 20191030
PL  - Netherlands
TA  - Fish Physiol Biochem
JT  - Fish physiology and biochemistry
JID - 100955049
RN  - 0 (Ghrelin)
SB  - IM
MH  - Animals
MH  - Brain/*physiology
MH  - Clustered Regularly Interspaced Short Palindromic Repeats
MH  - Ghrelin/*genetics
MH  - Transcriptome
MH  - Zebrafish/*genetics
OTO - NOTNLM
OT  - CRISPR/Cas9
OT  - Fish
OT  - Ghrelinergic system
OT  - Knockdown
OT  - RNA-seq
OT  - Transcriptomic
EDAT- 2019/11/02 06:00
MHDA- 2020/10/24 06:00
CRDT- 2019/11/02 06:00
PHST- 2019/05/28 00:00 [received]
PHST- 2019/07/17 00:00 [accepted]
PHST- 2019/11/02 06:00 [pubmed]
PHST- 2020/10/24 06:00 [medline]
PHST- 2019/11/02 06:00 [entrez]
AID - 10.1007/s10695-019-00687-6 [pii]
AID - 10.1007/s10695-019-00687-6 [doi]
PST - ppublish
SO  - Fish Physiol Biochem. 2020 Feb;46(1):1-21. doi: 10.1007/s10695-019-00687-6. Epub 
      2019 Oct 30.