PMID- 31676250
OWN - NLM
STAT- MEDLINE
DCOM- 20210618
LR  - 20210618
IS  - 1879-1409 (Electronic)
IS  - 0305-4179 (Linking)
VI  - 46
IP  - 3
DP  - 2020 May
TI  - Tim4 regulates NALP3 inflammasome expression and activity during 
      monocyte/macrophage dysfunction in septic shock patients.
PG  - 652-662
LID - S0305-4179(19)30267-0 [pii]
LID - 10.1016/j.burns.2019.08.016 [doi]
AB  - Sepsis is the leading cause of death in burn patients. Monocytes/macrophages 
      rapidly exhibit impaired production of proinflammatory cytokines and an elevated 
      generation of anti-inflammatory cytokines in septic patients with 
      immunosuppression. However, the expression patterns of Tim4 and Nod-like receptor 
      protein 3 (NALP3) inflammasome and their roles during immunosuppression in septic 
      shock patients are not well understood. Tim4 and NALP3 inflammasome expression in 
      monocytes were downregulated in immunosuppressive patients with sepsis compared 
      with healthy volunteers. Meanwhile, NALP3 inflammasome expression was upregulated 
      by Tim4 overexpression in murine bone marrow-derived macrophages (BMDMs) and 
      J774A.1 macrophages. Tim4 overexpression improved the ability of BMDMs and 
      J774A.1 macrophages to produce proinflammatory cytokines and increased the 
      expression of cleaved-caspase-1 (p10) after LPS/ATP stimulation. In addition, 
      overexpression of Tim4 enhanced phagocytosis of apoptotic polymorphonuclear 
      neutrophils (PMNs) by BMDMs and J774A.1 macrophages, while depletion of NALP3 in 
      Tim4 overexpressing BMDMs and J774A.1 macrophages decreased phagocytosis of 
      apoptotic PMNs. In summary, the expression of Tim4 and NALP3 inflammasome in 
      monocytes/macrophages was downregulated in septic shock patients, and diminished 
      expression of Tim4 and NALP3 inflammasome in monocytes/macrophages might play a 
      critical role in sepsis-elicited immunosuppression.
CI  - Copyright (c) 2019 Elsevier Ltd and ISBI. All rights reserved.
FAU - Liu, Zheng
AU  - Liu Z
AD  - Faculty of Anesthesiology, Changhai Hospital, Second Military Medical University, 
      200433, Shanghai, China.
FAU - Tan, Kezhe
AU  - Tan K
AD  - Faculty of Anesthesiology, Changhai Hospital, Second Military Medical University, 
      200433, Shanghai, China.
FAU - Bu, Lan
AU  - Bu L
AD  - Faculty of Anesthesiology, Changhai Hospital, Second Military Medical University, 
      200433, Shanghai, China.
FAU - Bo, Lulong
AU  - Bo L
AD  - Faculty of Anesthesiology, Changhai Hospital, Second Military Medical University, 
      200433, Shanghai, China.
FAU - Ni, Wen
AU  - Ni W
AD  - Faculty of Anesthesiology, Changhai Hospital, Second Military Medical University, 
      200433, Shanghai, China.
FAU - Fei, Miaomiao
AU  - Fei M
AD  - Faculty of Anesthesiology, Changhai Hospital, Second Military Medical University, 
      200433, Shanghai, China.
FAU - Chen, Fang
AU  - Chen F
AD  - Faculty of Anesthesiology, Changhai Hospital, Second Military Medical University, 
      200433, Shanghai, China.
FAU - Deng, Xiaoming
AU  - Deng X
AD  - Faculty of Anesthesiology, Changhai Hospital, Second Military Medical University, 
      200433, Shanghai, China. Electronic address: deng_x@yahoo.com.
FAU - Li, Jinbao
AU  - Li J
AD  - Department of Anesthesiology, Shanghai First People's Hospital, Jiaotong 
      University, 200081, Shanghai, China. Electronic address: 
      lijinbaoshanghai@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191030
PL  - Netherlands
TA  - Burns
JT  - Burns : journal of the International Society for Burn Injuries
JID - 8913178
RN  - 0 (Cytokines)
RN  - 0 (Inflammasomes)
RN  - 0 (Membrane Proteins)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (NLRP3 protein, human)
RN  - 0 (Nlrp3 protein, mouse)
RN  - 0 (TIM-4 protein, mouse)
RN  - 0 (TIMD4 protein, human)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Apoptosis
MH  - Burns/immunology/metabolism
MH  - Case-Control Studies
MH  - Caspase 1/metabolism
MH  - Cytokines/metabolism
MH  - Down-Regulation
MH  - Female
MH  - Humans
MH  - Immunocompromised Host/*immunology
MH  - Inflammasomes/immunology
MH  - Inflammation
MH  - Macrophages/*immunology/metabolism
MH  - Male
MH  - Membrane Proteins/*immunology/metabolism
MH  - Mice
MH  - Middle Aged
MH  - Monocytes/*immunology/metabolism
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/*immunology/metabolism
MH  - Neutrophils/immunology
MH  - Phagocytosis/immunology
MH  - Shock, Septic/*immunology/metabolism
MH  - Up-Regulation
OTO - NOTNLM
OT  - Burn patients
OT  - NALP3 inflammasome
OT  - Sepsis
OT  - Tim4
EDAT- 2019/11/05 06:00
MHDA- 2021/06/22 06:00
CRDT- 2019/11/03 06:00
PHST- 2019/04/20 00:00 [received]
PHST- 2019/08/28 00:00 [revised]
PHST- 2019/08/29 00:00 [accepted]
PHST- 2019/11/05 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2019/11/03 06:00 [entrez]
AID - S0305-4179(19)30267-0 [pii]
AID - 10.1016/j.burns.2019.08.016 [doi]
PST - ppublish
SO  - Burns. 2020 May;46(3):652-662. doi: 10.1016/j.burns.2019.08.016. Epub 2019 Oct 
      30.